Product: MSH6 Antibody
Catalog: DF7566
Source: Rabbit
Application: WB, IHC, IF/ICC, ELISA(peptide)
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 163 kD.; 153kD(Calculated).
Uniprot: P52701
RRID: AB_2841059

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(88%), Sheep(88%), Rabbit(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
MSH6 Antibody detects endogenous levels of total MSH6.
RRID:
AB_2841059
Cite Format: Affinity Biosciences Cat# DF7566, RRID:AB_2841059.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DNA mismatch repair protein Msh6; G/T mismatch binding protein; G/T mismatch-binding protein; GTBP; GTMBP; hMSH6; HNPCC 5; HNPCC5; HSAP; MSH 6; MSH6; MSH6_HUMAN; mutS (E. coli) homolog 6; MutS alpha 160 kDa subunit; MutS homolog 6 (E. coli); mutS homolog 6; MutS-alpha 160 kDa subunit; p160; Sperm associated protein;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MSRQSTLYSFFPKSPALSDANKASARASREGGRAAAAPGASPSPGGDAAWSEAGPGPRPLARSASPPKAKNLNGGLRRSVAPAAPTSCDFSPGDLVWAKMEGYPWWPCLVYNHPFDGTFIREKGKSVRVHVQFFDDSPTRGWVSKRLLKPYTGSKSKEAQKGGHFYSAKPEILRAMQRADEALNKDKIKRLELAVCDEPSEPEEEEEMEVGTTYVTDKSEEDNEIESEEEVQPKTQGSRRSSRQIKKRRVISDSESDIGGSDVEFKPDTKEEGSSDEISSGVGDSESEGLNSPVKVARKRKRMVTGNGSLKRKSSRKETPSATKQATSISSETKNTLRAFSAPQNSESQAHVSGGGDDSSRPTVWYHETLEWLKEEKRRDEHRRRPDHPDFDASTLYVPEDFLNSCTPGMRKWWQIKSQNFDLVICYKVGKFYELYHMDALIGVSELGLVFMKGNWAHSGFPEIAFGRYSDSLVQKGYKVARVEQTETPEMMEARCRKMAHISKYDRVVRREICRIITKGTQTYSVLEGDPSENYSKYLLSLKEKEEDSSGHTRAYGVCFVDTSLGKFFIGQFSDDRHCSRFRTLVAHYPPVQVLFEKGNLSKETKTILKSSLSCSLQEGLIPGSQFWDASKTLRTLLEEEYFREKLSDGIGVMLPQVLKGMTSESDSIGLTPGEKSELALSALGGCVFYLKKCLIDQELLSMANFEEYIPLDSDTVSTTRSGAIFTKAYQRMVLDAVTLNNLEIFLNGTNGSTEGTLLERVDTCHTPFGKRLLKQWLCAPLCNHYAINDRLDAIEDLMVVPDKISEVVELLKKLPDLERLLSKIHNVGSPLKSQNHPDSRAIMYEETTYSKKKIIDFLSALEGFKVMCKIIGIMEEVADGFKSKILKQVISLQTKNPEGRFPDLTVELNRWDTAFDHEKARKTGLITPKAGFDSDYDQALADIRENEQSLLEYLEKQRNRIGCRTIVYWGIGRNRYQLEIPENFTTRNLPEEYELKSTKKGCKRYWTKTIEKKLANLINAEERRDVSLKDCMRRLFYNFDKNYKDWQSAVECIAVLDVLLCLANYSRGGDGPMCRPVILLPEDTPPFLELKGSRHPCITKTFFGDDFIPNDILIGCEEEEQENGKAYCVLVTGPNMGGKSTLMRQAGLLAVMAQMGCYVPAEVCRLTPIDRVFTRLGASDRIMSGESTFFVELSETASILMHATAHSLVLVDELGRGTATFDGTAIANAVVKELAETIKCRTLFSTHYHSLVEDYSQNVAVRLGHMACMVENECEDPSQETITFLYKFIKGACPKSYGFNAARLANLPEEVIQKGHRKAREFEKMNQSLRLFREVCLASERSTVDAEAVHKLLTLIKEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
88
Sheep
88
Dog
88
Rabbit
88
Pig
0
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P52701 As Substrate

Site PTM Type Enzyme
Y8 Phosphorylation
S9 Phosphorylation
S14 Phosphorylation
K22 Ubiquitination
R33 Methylation
S41 Phosphorylation
S43 Phosphorylation
S51 Phosphorylation
S63 Phosphorylation
S65 Phosphorylation
K70 Acetylation
S79 Phosphorylation
T86 Phosphorylation
S87 Phosphorylation
S91 Phosphorylation
S137 Phosphorylation
T139 Phosphorylation
R140 Methylation
S144 Phosphorylation
Y151 Phosphorylation
K155 Acetylation
K161 Ubiquitination
K169 Sumoylation
K169 Ubiquitination
S200 Phosphorylation
T212 Phosphorylation
T213 Phosphorylation
Y214 Phosphorylation
T216 Phosphorylation
S219 Phosphorylation
S227 Phosphorylation
T235 Phosphorylation
S252 Phosphorylation
S254 Phosphorylation
S256 Phosphorylation
S261 Phosphorylation
T269 Phosphorylation
S274 Phosphorylation
S275 Phosphorylation
S279 Phosphorylation
S280 Phosphorylation
S285 Phosphorylation
S292 Phosphorylation
T305 Phosphorylation
S309 Phosphorylation
K324 Ubiquitination
K334 Sumoylation
K334 Ubiquitination
S348 Phosphorylation
K374 Ubiquitination
K476 Ubiquitination
K479 Ubiquitination
T488 Phosphorylation
K498 Ubiquitination
K504 Acetylation
K504 Ubiquitination
K519 Ubiquitination
K537 Ubiquitination
K543 Ubiquitination
R577 Methylation
K598 Ubiquitination
K603 Ubiquitination
K610 Ubiquitination
K632 Ubiquitination
Y642 Phosphorylation
K646 Ubiquitination
K660 Ubiquitination
S668 Phosphorylation
K693 Ubiquitination
S702 Phosphorylation
Y709 Phosphorylation
K728 Ubiquitination
T767 Phosphorylation
K771 Ubiquitination
K813 Ubiquitination
K814 Ubiquitination
K824 Ubiquitination
S830 Phosphorylation
K833 Methylation
K833 Ubiquitination
K852 Ubiquitination
K854 Ubiquitination
K866 Ubiquitination
K870 Ubiquitination
K883 Ubiquitination
K888 Ubiquitination
T895 Phosphorylation
K896 Ubiquitination
T914 Phosphorylation
K920 Ubiquitination
K923 Ubiquitination
T924 Phosphorylation
T928 Phosphorylation
K930 Ubiquitination
S935 Phosphorylation
K957 Ubiquitination
K997 Sumoylation
K997 Ubiquitination
K1009 Ubiquitination
T1010 Phosphorylation
K1014 Ubiquitination
K1030 Ubiquitination
T1085 Phosphorylation
K1092 Methylation
K1092 Ubiquitination
K1101 Ubiquitination
K1126 Methylation
K1140 Ubiquitination
R1172 Methylation
K1240 Ubiquitination
K1291 Ubiquitination
K1296 Ubiquitination
K1315 Ubiquitination
K1325 Ubiquitination
K1352 Ubiquitination
K1358 Ubiquitination

Research Backgrounds

Function:

Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with MSH2 to form MutS alpha, which binds to DNA mismatches thereby initiating DNA repair. When bound, MutS alpha bends the DNA helix and shields approximately 20 base pairs, and recognizes single base mismatches and dinucleotide insertion-deletion loops (IDL) in the DNA. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions. The ATPase activity associated with MutS alpha regulates binding similar to a molecular switch: mismatched DNA provokes ADP-->ATP exchange, resulting in a discernible conformational transition that converts MutS alpha into a sliding clamp capable of hydrolysis-independent diffusion along the DNA backbone. This transition is crucial for mismatch repair. MutS alpha may also play a role in DNA homologous recombination repair. Recruited on chromatin in G1 and early S phase via its PWWP domain that specifically binds trimethylated 'Lys-36' of histone H3 (H3K36me3): early recruitment to chromatin to be replicated allowing a quick identification of mismatch repair to initiate the DNA mismatch repair reaction.

PTMs:

The N-terminus is blocked.

Phosphorylated by PRKCZ, which may prevent MutS alpha degradation by the ubiquitin-proteasome pathway.

Subcellular Location:

Nucleus. Chromosome.
Note: Associates with H3K36me3 via its PWWP domain.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the DNA mismatch repair (MMR) complex composed at least of MSH2, MSH3, MSH6, PMS1 and MLH1. Heterodimer consisting of MSH2-MSH6 (MutS alpha). Forms a ternary complex with MutL alpha (MLH1-PMS1). Interacts with MCM9. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBS1 protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains.

(Microbial infection) Interacts with herpes simplex virus 1 protein UL12.

Family&Domains:

The PWWP domain specifically recognizes and binds trimethylated 'Lys-36' of histone H3 (H3K36me3).

Belongs to the DNA mismatch repair MutS family.

Research Fields

· Genetic Information Processing > Replication and repair > Mismatch repair.

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Colorectal cancer.   (View pathway)

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