Casein Kinase 2 beta Antibody - #DF7562

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Product: Casein Kinase 2 beta Antibody
Catalog: DF7562
Description: Rabbit polyclonal antibody to Casein Kinase 2 beta
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 25 kDa; 25kD(Calculated).
Uniprot: P67870
RRID: AB_2841056

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Casein Kinase 2 beta Antibody detects endogenous levels of total Casein Kinase 2 beta.
RRID:
AB_2841056
Cite Format: Affinity Biosciences Cat# DF7562, RRID:AB_2841056.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Casein kinase 2 beta polypeptide; Casein kinase II beta subunit; Casein kinase II subunit beta; CK II beta; CK2B; CK2N; CSK2B; CSK2B_HUMAN; CSNK 2B; csnk2b; G5A; MGC138222; MGC138224; Phosvitin; Protein G5a;

Immunogens

Immunogen:
Uniprot:

P67870(CSK2B_HUMAN) >>Visit HPA database.

Gene(ID):
CSNK2B(1460)
Sequence:
MSSSEEVSWISWFCGLRGNEFFCEVDEDYIQDKFNLTGLNEQVPHYRQALDMILDLEPDEELEDNPNQSDLIEQAAEMLYGLIHARYILTNRGIAQMLEKYQQGDFGYCPRVYCENQPMLPIGLSDIPGEAMVKLYCPKCMDVYTPKSSRHHHTDGAYFGTGFPHMLFMVHPEYRPKRPANQFVPRLYGFKIHPMAYQLQLQAASNFKSPVKTIR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P67870 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation P19784 (CSNK2A2) , P68400 (CSNK2A1) , P67870 (CSNK2B)
S3 Phosphorylation P68400 (CSNK2A1) , P19784 (CSNK2A2) , P67870 (CSNK2B)
S4 Phosphorylation
S8 Phosphorylation
S11 Phosphorylation
K33 Ubiquitination
T37 Phosphorylation
S69 Phosphorylation
R92 Methylation
K100 Ubiquitination
Y108 Phosphorylation
K134 Ubiquitination
K139 Acetylation
K139 Ubiquitination
Y144 Phosphorylation
T145 Phosphorylation
K147 Acetylation
K147 Ubiquitination
S148 Phosphorylation
S149 Phosphorylation
K177 Ubiquitination
R178 Methylation
R186 Methylation
K191 Ubiquitination
Y197 Phosphorylation
S205 Phosphorylation
K208 Methylation
K208 Ubiquitination
S209 Phosphorylation P06493 (CDK1)
K212 Acetylation
K212 Sumoylation
K212 Ubiquitination
T213 Phosphorylation O14757 (CHEK1)

PTMs - P67870 As Enzyme

Substrate Site Source
O95863 (SNAI1) S92 Uniprot
P04637-1 (TP53) T18 Uniprot
P08473 (MME) S6 Uniprot
P17509-1 (HOXB6) S214 Uniprot
P35222 (CTNNB1) S29 Uniprot
P35222 (CTNNB1) T102 Uniprot
P35222 (CTNNB1) T112 Uniprot
P35568 (IRS1) S24 Uniprot
P35568 (IRS1) T88 Uniprot
P35568 (IRS1) S99 Uniprot
P35568 (IRS1) S330 Uniprot
P35568 (IRS1) T811 Uniprot
P38398-3 (BRCA1) S468 Uniprot
P38398-8 (BRCA1) S1525 Uniprot
P49427 (CDC34) S203 Uniprot
P49427 (CDC34) S222 Uniprot
P49427 (CDC34) S231 Uniprot
P49427 (CDC34) T233 Uniprot
P49427 (CDC34) S236 Uniprot
P55010 (EIF5) S174 Uniprot
P55010 (EIF5) S389 Uniprot
P55010 (EIF5) S390 Uniprot
P55957-1 (BID) T59 Uniprot
P55957 (BID) S64 Uniprot
P67870 (CSNK2B) S2 Uniprot
P67870 (CSNK2B) S3 Uniprot
Q01105-2 (SET) S9 Uniprot
Q13158 (FADD) S200 Uniprot
Q13422 (IKZF1) S13 Uniprot
Q13422 (IKZF1) T23 Uniprot
Q13422 (IKZF1) S63 Uniprot
Q13422 (IKZF1) S101 Uniprot
Q13422 (IKZF1) S295 Uniprot
Q16625-1 (OCLN) T404 Uniprot
Q16625-1 (OCLN) S408 Uniprot
Q99523 (SORT1) S825 Uniprot
Q9UBF6-3 (RNF7) T10 Uniprot
Q9Y5B0-4 (CTDP1) S575 Uniprot
Q9Y5B0 (CTDP1) S740 Uniprot

Research Backgrounds

Function:

Participates in Wnt signaling (By similarity). Plays a complex role in regulating the basal catalytic activity of the alpha subunit.

PTMs:

Phosphorylated by alpha subunit.

Subunit Structure:

Tetramer composed of an alpha subunit, an alpha' subunit and two beta subunits. The beta subunit dimerization is mediated by zinc ions. Interacts with TCTEX1D3 (By similarity). Interacts with CD163. Also component of a CK2-SPT16-SSRP1 complex composed of SSRP1, SUPT16H, CSNK2A1, CSNK2A2 and CSNK2B, the complex associating following UV irradiation. Interacts with MUSK; mediates phosphorylation of MUSK by CK2. Interacts with FGF1; this interaction is increased in the presence of FIBP, suggesting a possible cooperative interaction between CSNKB and FIBP in binding to FGF1.

Family&Domains:

Belongs to the casein kinase 2 subunit beta family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Adherens junction.   (View pathway)

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

· Human Diseases > Infectious diseases: Viral > Measles.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Infectious diseases: Viral > Epstein-Barr virus infection.

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