PAX6 Antibody - #DF7557

(1)   (3)  
Product: PAX6 Antibody
Catalog: DF7557
Description: Rabbit polyclonal antibody to PAX6
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 47 kDa; 47kD(Calculated).
Uniprot: P26367
RRID: AB_2841051

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IF/ICC 1:100-1:500, WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PAX6 Antibody detects endogenous levels of total PAX6.
RRID:
AB_2841051
Cite Format: Affinity Biosciences Cat# DF7557, RRID:AB_2841051.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AN 2; AN; AN2; Aniridia type II protein; D11S812E; FVH1; KIAA0552; Leucine zipper putative tumor suppressor 3; LZTS3; MGC17209; MGDA; Oculorhombin; Paired box 6; Paired box gene 6 (aniridia keratitis); Paired Box Gene 6; Paired box homeotic gene 6; Paired box protein Pax-6; Paired box protein Pax6; PAX 6; PAX6; PAX6_HUMAN; ProSAP-interacting protein 1; PROSAPIP1; Sey; WAGR;

Immunogens

Immunogen:
Uniprot:

P26367(PAX6_HUMAN) >>Visit HPA database.

Gene(ID):
PAX6(5080)
Expression:
P26367 PAX6_HUMAN:

Fetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form.

Sequence:
MQNSHSGVNQLGGVFVNGRPLPDSTRQKIVELAHSGARPCDISRILQVSNGCVSKILGRYYETGSIRPRAIGGSKPRVATPEVVSKIAQYKRECPSIFAWEIRDRLLSEGVCTNDNIPSVSSINRVLRNLASEKQQMGADGMYDKLRMLNGQTGSWGTRPGWYPGTSVPGQPTQDGCQQQEGGGENTNSISSNGEDSDEAQMRLQLKRKLQRNRTSFTQEQIEALEKEFERTHYPDVFARERLAAKIDLPEARIQVWFSNRRAKWRREEKLRNQRRQASNTPSHIPISSSFSTSVYQPIPQPTTPVSSFTSGSMLGRTDTALTNTYSALPPMPSFTMANNLPMQPPVPSQTSSYSCMLPTSPSVNGRSYDTYTPPHMQTHMNSQPMGTSGTTSTGLISPGVSVPVQVPGSEPDMSQYWPRLQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P26367 As Substrate

Site PTM Type Enzyme
K55 Ubiquitination
S216 Phosphorylation
T281 Phosphorylation Q9H2X6 (HIPK2)
T304 Phosphorylation Q9H2X6 (HIPK2)
T373 Phosphorylation Q9H2X6 (HIPK2)

Research Backgrounds

Function:

Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Isoform 5a appears to function as a molecular switch that specifies target genes.

PTMs:

Ubiquitinated by TRIM11, leading to ubiquitination and proteasomal degradation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Fetal eye, brain, spinal cord and olfactory epithelium. Isoform 5a is less abundant than the PAX6 shorter form.

Subunit Structure:

Interacts with MAF and MAFB. Interacts with TRIM11; this interaction leads to ubiquitination and proteasomal degradation, as well as inhibition of transactivation, possibly in part by preventing PAX6 binding to consensus DNA sequences.

Family&Domains:

Belongs to the paired homeobox family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.

References

1). Long non-coding RNA (lncRNA) PGM5P4-AS1 inhibits lung cancer progression by up-regulating Leucine Zipper Tumor Suppressor (LZTS3) through sponging microRNA miR-1275. Bioengineered, 2020 (PubMed: 33315502) [IF=4.9]

Application: WB    Species: Human    Sample: A549 cells

Figure 5. PGM5P4-AS1 decreased the miR-1275 level and promoted the expression of LZTS3. (a-b), Relative expression levels of miRNA-1275 and LZTS3 in A549 cells, that were interfered in PGM5P4-AS1 expression (**p < 0.01 vs. NC siRNA). (c-d), Relative expression levels of miRNA-1275 and LZTS3 in SK-MES-1 cells, that were overexpressed of PGM5P4-AS1 (##p < 0.01 vs. vector). (e-f), Protein expression levels of LZTS3, cyclinD1, MMP-2, Bcl-2, and Bcl-xl in A549 cells, that were transfected with PGM5P4-AS1 siRNA or PGM5P4-AS1-OE, respectively (**p < 0.01 vs. NC siRNA, ##p < 0.01 vs. vector).

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.