Product: RAB7 Antibody
Catalog: DF7546
Description: Rabbit polyclonal antibody to RAB7
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 23 kDa; 23kD(Calculated).
Uniprot: P51149
RRID: AB_2841045

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(88%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
RAB7 Antibody detects endogenous levels of total RAB7.
RRID:
AB_2841045
Cite Format: Affinity Biosciences Cat# DF7546, RRID:AB_2841045.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CMT2B; PRO2706; PSN; RAB7, member RAS oncogene family; RAB7A; RAB7A, member RAS oncogene family; RAB7A_HUMAN; Ras associated protein RAB7; Ras related protein Rab7; Ras related protein Rab7a; Ras-related protein Rab-7a;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P51149 RAB7A_HUMAN:

Widely expressed; high expression found in skeletal muscle.

Sequence:
MTSRKKVLLKVIILGDSGVGKTSLMNQYVNKKFSNQYKATIGADFLTKEVMVDDRLVTMQIWDTAGQERFQSLGVAFYRGADCCVLVFDVTAPNTFKTLDSWRDEFLIQASPRDPENFPFVVLGNKIDLENRQVATKRAQAWCYSKNNIPYFETSAKEAINVEQAFQTIARNALKQETEVELYNEFPEPIKLDKNDRAKASAESCSC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Bovine
88
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51149 As Substrate

Site PTM Type Enzyme
T2 Acetylation
S17 Phosphorylation
K21 Ubiquitination
Y28 Phosphorylation
K31 Ubiquitination
K32 Ubiquitination
Y37 Phosphorylation
K38 Ubiquitination
T40 Phosphorylation
K48 Ubiquitination
R55 Methylation
S72 Phosphorylation
C83 S-Nitrosylation
C84 S-Nitrosylation
K97 Ubiquitination
S101 Phosphorylation
S111 Phosphorylation
K126 Ubiquitination
R132 Methylation
K137 Ubiquitination
K146 Ubiquitination
K157 Ubiquitination
T168 Phosphorylation
K175 Ubiquitination
T178 Phosphorylation
Y183 Phosphorylation
K191 Ubiquitination
K194 Ubiquitination
S201 Phosphorylation
S204 Phosphorylation
S206 Phosphorylation

Research Backgrounds

Function:

Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and M.tuberculosis. Plays a role in the fusion of phagosomes with lysosomes. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation. Involved in the ADRB2-stimulated lipolysis through lipophagy, a cytosolic lipase-independent autophagic pathway (By similarity). Required for the exosomal release of SDCBP, CD63 and syndecan.

Subcellular Location:

Cytoplasmic vesicle>Phagosome membrane>Peripheral membrane protein>Cytoplasmic side. Late endosome membrane>Peripheral membrane protein>Cytoplasmic side. Lysosome membrane>Peripheral membrane protein>Cytoplasmic side. Melanosome membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle>Autophagosome membrane>Peripheral membrane protein>Cytoplasmic side. Lipid droplet. Endosome membrane.
Note: Colocalizes with OSBPL1A at the late endosome (PubMed:16176980). Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Recruited to phagosomes containing S.aureus or Mycobacterium (PubMed:21255211). Lipid droplet localization is increased upon ADRB2 stimulation (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed; high expression found in skeletal muscle.

Subunit Structure:

The GTP-bound form interacts with RAC1 (By similarity). Interacts with NTRK1/TRKA (By similarity). Interacts with C9orf72 (By similarity). Interacts with CHM, the substrate-binding subunit of the Rab geranylgeranyltransferase complex (By similarity). Interacts with RILP. Interacts with PSMA7. Interacts with RNF115. Interacts with FYCO1. Interacts with the PIK3C3/VPS34-PIK3R4 complex. The GTP-bound form interacts with OSBPL1A. Interacts with CLN3. Does not interact with HPS4 and the BLOC-3 complex (heterodimer of HPS1 and HPS4). Interacts with CLN5. Interacts with PLEKHM1 (via N- and C-terminus) (By similarity). Interacts with RUFY4.

Family&Domains:

Belongs to the small GTPase superfamily. Rab family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.