Catalase Antibody - #DF7545

(3)   (6)  
Product: Catalase Antibody
Catalog: DF7545
Description: Rabbit polyclonal antibody to Catalase
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 60 kDa; 60kD(Calculated).
Uniprot: P04040
RRID: AB_2841044

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(83%), Sheep(83%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Catalase Antibody detects endogenous levels of total Catalase.
RRID:
AB_2841044
Cite Format: Affinity Biosciences Cat# DF7545, RRID:AB_2841044.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Cas1; CAT; CATA_HUMAN; Catalase; Cs1; MGC138422; MGC138424;

Immunogens

Immunogen:
Uniprot:

P04040(CATA_HUMAN) >>Visit HPA database.

Gene(ID):
CAT(847)
Sequence:
MADSRDPASDQMQHWKEQRAAQKADVLTTGAGNPVGDKLNVITVGPRGPLLVQDVVFTDEMAHFDRERIPERVVHAKGAGAFGYFEVTHDITKYSKAKVFEHIGKKTPIAVRFSTVAGESGSADTVRDPRGFAVKFYTEDGNWDLVGNNTPIFFIRDPILFPSFIHSQKRNPQTHLKDPDMVWDFWSLRPESLHQVSFLFSDRGIPDGHRHMNGYGSHTFKLVNANGEAVYCKFHYKTDQGIKNLSVEDAARLSQEDPDYGIRDLFNAIATGKYPSWTFYIQVMTFNQAETFPFNPFDLTKVWPHKDYPLIPVGKLVLNRNPVNYFAEVEQIAFDPSNMPPGIEASPDKMLQGRLFAYPDTHRHRLGPNYLHIPVNCPYRARVANYQRDGPMCMQDNQGGAPNYYPNSFGAPEQQPSALEHSIQYSGEVRRFNTANDDNVTQVRAFYVNVLNEEQRKRLCENIAGHLKDAQIFIQKKAVKNFTEVHPDYGSHIQALLDKYNAEKPKNAIHTFVQSGSHLAAREKANL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Dog
100
Bovine
83
Sheep
83
Pig
75
Zebrafish
58
Horse
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P04040 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S9 Phosphorylation
K16 Ubiquitination
K23 Ubiquitination
K38 Acetylation
K38 Ubiquitination
R47 Methylation
K77 Ubiquitination
Y84 Phosphorylation
K98 Ubiquitination
K105 Ubiquitination
T107 Phosphorylation
S114 O-Glycosylation
T115 Phosphorylation
T150 Phosphorylation
S167 Phosphorylation Q05655 (PRKCD)
Y231 Phosphorylation P42684 (ABL2) , P00519 (ABL1) , A0A173G4P4 (Abl fusion)
C232 S-Nitrosylation
K233 Ubiquitination
K237 Acetylation
K237 Ubiquitination
K243 Ubiquitination
S254 O-Glycosylation
Y260 Phosphorylation
K306 Ubiquitination
Y308 Phosphorylation
K315 Ubiquitination
Y358 Phosphorylation
Y370 Phosphorylation
Y379 Phosphorylation
Y386 Phosphorylation A0A173G4P4 (Abl fusion) , P42684 (ABL2) , P00519 (ABL1)
S422 Phosphorylation
Y425 Phosphorylation
Y447 Phosphorylation
C460 S-Nitrosylation
K468 Acetylation
K468 Ubiquitination
K476 Acetylation
K480 Acetylation
K480 Ubiquitination
K499 Ubiquitination
T511 Phosphorylation
S515 Phosphorylation
S517 Phosphorylation

Research Backgrounds

Function:

Occurs in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. Promotes growth of cells including T-cells, B-cells, myeloid leukemia cells, melanoma cells, mastocytoma cells and normal and transformed fibroblast cells.

PTMs:

The N-terminus is blocked.

Subcellular Location:

Peroxisome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotetramer.

Family&Domains:

Belongs to the catalase family.

Research Fields

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway.   (View pathway)

· Human Diseases > Neurodegenerative diseases > Amyotrophic lateral sclerosis (ALS).

· Metabolism > Amino acid metabolism > Tryptophan metabolism.

· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.

· Metabolism > Global and overview maps > Carbon metabolism.

· Organismal Systems > Aging > Longevity regulating pathway.   (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species.   (View pathway)

References

1). The TβR II-targeted aptamer S58 prevents fibrosis after glaucoma filtration surgery. Aging-US, 2020 (PubMed: 32452828) [IF=5.2]

Application: WB    Species: human    Sample: HConFs

Figure 5. | S58 promotes antioxidant defense of TGF-β2-induced HConFs. (A) Analysis of antioxidant capacity of cells at day 14 after GFS. (B) Intracellular ROS variation, and (C) mitochondrial superoxide variation were examined by flow cytometry. (D) Antioxidant protein SOD1/2, γ-GCS and CAT levels analyzed by western blotting in TGF-β2-treated HConFs in the presence or absence of S58 (20 nM). (E) Relative antioxidant gene levels in HConFs preconditioned with TGF-β2 in the presence or absence of S58 (20 nM) for 12h. All data indicate the mean ± SD, n=3. *p < 0.05, **p < 0.01, ***p < 0.001.
2). Triptolide promotes ferroptosis by suppressing Nrf2 to overcome leukemia cell resistance to doxorubicin. Molecular Medicine Reports, 2023 (PubMed: 36453238) [IF=3.4]
3). Cholecystokinin Octapeptide Promotes ANP Secretion through Activation of NOX4–PGC-1α–PPARα/PPARγ Signaling in Isolated Beating Rat Atria. Oxidative Medicine and Cellular Longevity, 2022 (PubMed: 35770043)

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.