DDX5 Antibody | Affinity Biosciences

1/2

Western blot analysis of DDX5 using HepG2 whole cell lysates.

DF7539 at 1/100 staining Rat heart tissue by IHC-P.

Product:	DDX5 Antibody
Catalog:	DF7539
Description:	Rabbit polyclonal antibody to DDX5
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.:	69 kDa; 69kD(Calculated).
Uniprot:	P17844
RRID:	AB_2841038

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

DDX5 Antibody detects endogenous levels of total DDX5.

RRID:

AB_2841038
Cite Format: Affinity Biosciences Cat# DF7539, RRID:AB_2841038.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ATP dependent RNA helicase DDX5; DDX 5; Ddx5; DDX5_HUMAN; DEAD (Asp Glu Ala Asp) box helicase 5; DEAD (Asp Glu Ala Asp) box polypeptide 5; DEAD box 5; DEAD box protein 5; DEAD/H (Asp Glu Ala Asp/His) box polypeptide 5 (RNA helicase, 68kD); G17P1; HELR; HLR1; HUMP68; P68; p68 RNA helicase; Probable ATP dependent RNA helicase DDX5; Probable ATP-dependent RNA helicase DDX5; RNA helicase p68;

Immunogens

Immunogen:

Uniprot:

P17844(DDX5_HUMAN) >>Visit HPA database.

Gene(ID):

DDX5(1655)

Sequence:

P17844 DDX5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSGYSSDRDRGRDRGFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPANVMDVIARQNFTEPTAIQAQGWPVALSGLDMVGVAQTGSGKTLSYLLPAIVHINHQPFLERGDGPICLVLAPTRELAQQVQQVAAEYCRACRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLECGKTNLRRTTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVRQLAEDFLKDYIHINIGALELSANHNILQIVDVCHDVEKDEKLIRLMEEIMSEKENKTIVFVETKRRCDELTRKMRRDGWPAMGIHGDKSQQERDWVLNEFKHGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSTKTGTAYTFFTPNNIKQVSDLISVLREANQAINPKLLQLVEDRGSGRSRGRGGMKDDRRDRYSAGKRGGFNTFRDRENYDRGYSSLLKRDFGAKTQNGVYSAANYTNGSFGSNFVSAGIQTSFRTGNPTGTYQNGYDSTQQYGSNVPNMHNGMNQQAYAYPATAAAPMIGYPMPTGYSQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

Chicken

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17844 As Substrate

P17844

Site	PTM Type	Enzyme	Source
S2	Phosphorylation		Uniprot
S5	Phosphorylation		Uniprot
S6	Phosphorylation		Uniprot
R10	Methylation		Uniprot
R12	Methylation		Uniprot
R14	Methylation		Uniprot
R20	Methylation		Uniprot
S24	Phosphorylation		Uniprot
R25	Methylation		Uniprot
S30	Phosphorylation		Uniprot
K32	Acetylation		Uniprot
K32	Ubiquitination		Uniprot
K33	Acetylation		Uniprot
K33	Ubiquitination		Uniprot
K40	Acetylation		Uniprot
K40	Ubiquitination		Uniprot
K43	Acetylation		Uniprot
K44	Acetylation		Uniprot
K45	Acetylation		Uniprot
K45	Sumoylation		Uniprot
K45	Ubiquitination		Uniprot
K53	Acetylation		Uniprot
K53	Sumoylation		Uniprot
K53	Ubiquitination		Uniprot
K56	Acetylation		Uniprot
K56	Methylation		Uniprot
K56	Sumoylation		Uniprot
K56	Ubiquitination		Uniprot
Y59	Phosphorylation		Uniprot
S79	Phosphorylation		Uniprot
K80	Acetylation		Uniprot
K80	Ubiquitination		Uniprot
T83	Phosphorylation		Uniprot
K91	Ubiquitination		Uniprot
Y97	Phosphorylation		Uniprot
K144	Ubiquitination		Uniprot
K197	Methylation		Uniprot
K197	Ubiquitination		Uniprot
S198	Phosphorylation	P49137 (MAPKAPK2)	Uniprot
T199	Phosphorylation		Uniprot
C200	S-Nitrosylation		Uniprot
Y202	Phosphorylation		Uniprot
K207	Sumoylation		Uniprot
K207	Ubiquitination		Uniprot
T224	Phosphorylation		Uniprot
C234	S-Nitrosylation		Uniprot
K236	Acetylation		Uniprot
K236	Ubiquitination		Uniprot
T243	Phosphorylation		Uniprot
Y244	Phosphorylation		Uniprot
R263	Methylation		Uniprot
K264	Acetylation		Uniprot
K264	Ubiquitination		Uniprot
R273	Methylation		Uniprot
S279	Phosphorylation		Uniprot
K284	Ubiquitination		Uniprot
Y297	Phosphorylation		Uniprot
K328	Ubiquitination		Uniprot
S338	Phosphorylation		Uniprot
K340	Acetylation		Uniprot
K340	Methylation		Uniprot
K340	Ubiquitination		Uniprot
K343	Ubiquitination		Uniprot
T344	Phosphorylation		Uniprot
K351	Acetylation		Uniprot
K351	Ubiquitination		Uniprot
K375	Ubiquitination		Uniprot
K388	Acetylation		Uniprot
K388	Ubiquitination		Uniprot
K391	Sumoylation		Uniprot
K391	Ubiquitination		Uniprot
S402	Phosphorylation		Uniprot
R403	Methylation		Uniprot
K411	Sumoylation		Uniprot
K411	Ubiquitination		Uniprot
Y416	Phosphorylation		Uniprot
S421	Phosphorylation		Uniprot
Y425	Phosphorylation		Uniprot
K437	Sumoylation		Uniprot
K437	Ubiquitination		Uniprot
T438	Phosphorylation		Uniprot
T440	Phosphorylation		Uniprot
Y442	Phosphorylation		Uniprot
T446	Phosphorylation	Q16539 (MAPK14)	Uniprot
K451	Ubiquitination		Uniprot
S454	Phosphorylation		Uniprot
S458	Phosphorylation		Uniprot
K470	Acetylation		Uniprot
K470	Sumoylation		Uniprot
K470	Ubiquitination		Uniprot
R478	Methylation		Uniprot
S480	Phosphorylation		Uniprot
R484	Methylation		Uniprot
R486	Methylation		Uniprot
K490	Methylation		Uniprot
S498	Phosphorylation		Uniprot
K501	Acetylation		Uniprot
R502	Methylation		Uniprot
T507	Phosphorylation		Uniprot
R509	Methylation		Uniprot
Y514	Phosphorylation		Uniprot
R516	Methylation		Uniprot
Y518	Phosphorylation		Uniprot
S519	Phosphorylation		Uniprot
S520	Phosphorylation		Uniprot
K523	Sumoylation		Uniprot
K523	Ubiquitination		Uniprot
Y535	Phosphorylation		Uniprot
S557	Phosphorylation		Uniprot
T564	Phosphorylation	Q16539 (MAPK14)	Uniprot
Y593	Phosphorylation	P00519 (ABL1)	Uniprot
Y595	Phosphorylation		Uniprot

Research Backgrounds

P17844_DDX5_HUMAN

Function:

Involved in the alternative regulation of pre-mRNA splicing; its RNA helicase activity is necessary for increasing tau exon 10 inclusion and occurs in a RBM4-dependent manner. Binds to the tau pre-mRNA in the stem-loop region downstream of exon 10. The rate of ATP hydrolysis is highly stimulated by single-stranded RNA. Involved in transcriptional regulation; the function is independent of the RNA helicase activity. Transcriptional coactivator for androgen receptor AR but probably not ESR1. Synergizes with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity and involved in skeletal muscle differentiation. Transcriptional coactivator for p53/TP53 and involved in p53/TP53 transcriptional response to DNA damage and p53/TP53-dependent apoptosis. Transcriptional coactivator for RUNX2 and involved in regulation of osteoblast differentiation. Acts as transcriptional repressor in a promoter-specific manner; the function probably involves association with histone deacetylases, such as HDAC1. As component of a large PER complex is involved in the inhibition of 3' transcriptional termination of circadian target genes such as PER1 and NR1D1 and the control of the circadian rhythms.

PTMs:

Arg-502 is dimethylated, probably to asymmetric dimethylarginine.

Sumoylated; sumoylation, promoted by PIAS1, promotes interaction with HDAC1 and transcriptional repression activity. Sumoylation also significantly increases stability, and reduces polyubiquitination.

Polyubiquitinated, leading to proteasomal degradation.

Subcellular Location:

Nucleus>Nucleolus.

Subunit Structure:

Identified in the spliceosome C complex. Interacts with RBM4; the interaction occurs in an RNA-independent manner. Interacts with AGO1 and AGO2. Interacts with ESR1, AR, EP300, CREBBP, POLR2A, TP53, RUNX2 and HDAC1. Self-associates. Interacts with DDX17. Interacts with BRDT. The large PER complex involved in the repression of transcriptional termination is composed of at least PER2, CDK9, DDX5, DHX9, NCBP1 and POLR2A (active). Interacts with DHX36; this interaction occurs in a RNA-dependent manner. Interacts with NUPR1 (By similarity). Interacts with ERCC6.

Family&Domains:

Belongs to the DEAD box helicase family. DDX5/DBP2 subfamily.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

DDX5 Antibody - #DF7539