IRS2 Antibody | Affinity Biosciences

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FIGURE 4 | Sleep restriction induced depletion of circadian clock expression.

Figure 11 Effect of YQ on insulin secretion-related proteins in pancreas islet.

Product:	IRS2 Antibody
Catalog:	DF7534
Description:	Rabbit polyclonal antibody to IRS2
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Dog
Mol.Wt.:	185 kDa; 137kD(Calculated).
Uniprot:	Q9Y4H2
RRID:	AB_2841033

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Dog(100%)

Clonality:

Polyclonal

Specificity:

IRS2 Antibody detects endogenous levels of total IRS2.

RRID:

AB_2841033
Cite Format: Affinity Biosciences Cat# DF7534, RRID:AB_2841033.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Insulin receptor substrate 2; IRS 2; IRS-2; Irs2; IRS2_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q9Y4H2(IRS2_HUMAN) >>Visit HPA database.

Gene(ID):

IRS2(8660)

Sequence:

Q9Y4H2 IRS2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASPPRHGPPGPASGDGPNLNNNNNNNNHSVRKCGYLRKQKHGHKRFFVLRGPGAGGDEATAGGGSAPQPPRLEYYESEKKWRSKAGAPKRVIALDCCLNINKRADAKHKYLIALYTKDEYFAVAAENEQEQEGWYRALTDLVSEGRAAAGDAPPAAAPAASCSASLPGALGGSAGAAGAEDSYGLVAPATAAYREVWQVNLKPKGLGQSKNLTGVYRLCLSARTIGFVKLNCEQPSVTLQLMNIRRCGHSDSFFFIEVGRSAVTGPGELWMQADDSVVAQNIHETILEAMKALKELFEFRPRSKSQSSGSSATHPISVPGARRHHHLVNLPPSQTGLVRRSRTDSLAATPPAAKCSSCRVRTASEGDGGAAAGAAAAGARPVSVAGSPLSPGPVRAPLSRSHTLSGGCGGRGSKVALLPAGGALQHSRSMSMPVAHSPPAATSPGSLSSSSGHGSGSYPPPPGPHPPLPHPLHHGPGQRPSSGSASASGSPSDPGFMSLDEYGSSPGDLRAFCSHRSNTPESIAETPPARDGGGGGEFYGYMTMDRPLSHCGRSYRRVSGDAAQDLDRGLRKRTYSLTTPARQRPVPQPSSASLDEYTLMRATFSGSAGRLCPSCPASSPKVAYHPYPEDYGDIEIGSHRSSSSNLGADDGYMPMTPGAALAGSGSGSCRSDDYMPMSPASVSAPKQILQPRAAAAAAAAVPSAGPAGPAPTSAAGRTFPASGGGYKASSPAESSPEDSGYMRMWCGSKLSMEHADGKLLPNGDYLNVSPSDAVTTGTPPDFFSAALHPGGEPLRGVPGCCYSSLPRSYKAPYTCGGDSDQYVLMSSPVGRILEEERLEPQATPGPSQAASAFGAGPTQPPHPVVPSPVRPSGGRPEGFLGQRGRAVRPTRLSLEGLPSLPSMHEYPLPPEPKSPGEYINIDFGEPGARLSPPAPPLLASAASSSSLLSASSPASSLGSGTPGTSSDSRQRSPLSDYMNLDFSSPKSPKPGAPSGHPVGSLDGLLSPEASSPYPPLPPRPSASPSSSLQPPPPPPAPGELYRLPPASAVATAQGPGAASSLSSDTGDNGDYTEMAFGVAATPPQPIAAPPKPEAARVASPTSGVKRLSLMEQVSGVEAFLQASQPPDPHRGAKVIRADPQGGRRRHSSETFSSTTTVTPVSPSFAHNPKRHNSASVENVSLRKSSEGGVGVGPGGGDEPPTSPRQLQPAPPLAPQGRPWTPGQPGGLVGCPGSGGSPMRRETSAGFQNGLNYIAIDVREEPGLPPQPQPPPPPLPQPGDKSSWGRTRSLGGLISAVGVGSTGGGCGGPGPGALPPANTYASIDFLSHHLKEATIVKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

100

Pig

Bovine

Horse

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y4H2 As Substrate

Q9Y4H2

Site	PTM Type	Enzyme	Source
	Phosphorylation		Uniprot
S3	Phosphorylation		Uniprot
S30	Phosphorylation		Uniprot
Y75	Phosphorylation		Uniprot
Y76	Phosphorylation		Uniprot
Y184	Phosphorylation		Uniprot
K295	Ubiquitination		Uniprot
S304	Phosphorylation		Uniprot
K305	Ubiquitination		Uniprot
S306	Phosphorylation	P05771 (PRKCB)	Uniprot
S308	Phosphorylation		Uniprot
S309	Phosphorylation		Uniprot
S311	Phosphorylation		Uniprot
S312	Phosphorylation		Uniprot
T314	Phosphorylation		Uniprot
S334	Phosphorylation		Uniprot
S342	Phosphorylation		Uniprot
T344	Phosphorylation		Uniprot
S346	Phosphorylation		Uniprot
T350	Phosphorylation	P45983 (MAPK8)	Uniprot
S358	Phosphorylation		Uniprot
T363	Phosphorylation		Uniprot
S365	Phosphorylation		Uniprot
S384	Phosphorylation		Uniprot
S388	Phosphorylation		Uniprot
S391	Phosphorylation	P06493 (CDK1)	Uniprot
S400	Phosphorylation		Uniprot
T404	Phosphorylation		Uniprot
S406	Phosphorylation		Uniprot
R412	Methylation		Uniprot
R429	Methylation		Uniprot
S430	Phosphorylation		Uniprot
S432	Phosphorylation		Uniprot
S491	Phosphorylation	P45983 (MAPK8)	Uniprot
S505	Phosphorylation		Uniprot
S506	Phosphorylation		Uniprot
S515	Phosphorylation		Uniprot
S518	Phosphorylation		Uniprot
T520	Phosphorylation		Uniprot
S523	Phosphorylation		Uniprot
T527	Phosphorylation		Uniprot
Y540	Phosphorylation		Uniprot
Y542	Phosphorylation		Uniprot
T544	Phosphorylation		Uniprot
S550	Phosphorylation		Uniprot
S560	Phosphorylation		Uniprot
R569	Methylation		Uniprot
T575	Phosphorylation		Uniprot
Y576	Phosphorylation		Uniprot
S577	Phosphorylation		Uniprot
T579	Phosphorylation		Uniprot
T580	Phosphorylation		Uniprot
S591	Phosphorylation		Uniprot
S592	Phosphorylation		Uniprot
S594	Phosphorylation		Uniprot
Y598	Phosphorylation		Uniprot
T599	Phosphorylation		Uniprot
T604	Phosphorylation		Uniprot
S606	Phosphorylation		Uniprot
S608	Phosphorylation		Uniprot
S615	Phosphorylation		Uniprot
S619	Phosphorylation		Uniprot
S620	Phosphorylation		Uniprot
Y625	Phosphorylation		Uniprot
Y628	Phosphorylation	P06213 (INSR)	Uniprot
Y632	Phosphorylation	P06213 (INSR)	Uniprot
S639	Phosphorylation		Uniprot
S642	Phosphorylation		Uniprot
S645	Phosphorylation		Uniprot
Y653	Phosphorylation		Uniprot
T657	Phosphorylation	P28482 (MAPK1)	Uniprot
S672	Phosphorylation		Uniprot
Y675	Phosphorylation		Uniprot
S679	Phosphorylation	P05771 (PRKCB)	Uniprot
S682	Phosphorylation		Uniprot
T713	Phosphorylation		Uniprot
S714	Phosphorylation		Uniprot
S723	Phosphorylation		Uniprot
S730	Phosphorylation		Uniprot
S731	Phosphorylation		Uniprot
S735	Phosphorylation		Uniprot
S736	Phosphorylation		Uniprot
S740	Phosphorylation		Uniprot
Y742	Phosphorylation		Uniprot
S752	Phosphorylation		Uniprot
Y766	Phosphorylation		Uniprot
S770	Phosphorylation		Uniprot
S772	Phosphorylation		Uniprot
T776	Phosphorylation		Uniprot
T777	Phosphorylation		Uniprot
T779	Phosphorylation		Uniprot
Y803	Phosphorylation		Uniprot
S804	Phosphorylation		Uniprot
S805	Phosphorylation		Uniprot
S809	Phosphorylation		Uniprot
Y810	Phosphorylation		Uniprot
Y814	Phosphorylation		Uniprot
T815	Phosphorylation		Uniprot
S820	Phosphorylation		Uniprot
Y823	Phosphorylation		Uniprot
S827	Phosphorylation		Uniprot
S828	Phosphorylation		Uniprot
T891	Phosphorylation		Uniprot
S894	Phosphorylation		Uniprot
S903	Phosphorylation		Uniprot
S915	Phosphorylation		Uniprot
Y919	Phosphorylation		Uniprot
S932	Phosphorylation		Uniprot
S973	Phosphorylation		Uniprot
S976	Phosphorylation		Uniprot
Y978	Phosphorylation		Uniprot
S985	Phosphorylation		Uniprot
T1082	Phosphorylation		Uniprot
S1100	Phosphorylation		Uniprot
S1103	Phosphorylation		Uniprot
S1109	Phosphorylation		Uniprot
K1134	Methylation		Uniprot
S1148	Phosphorylation		Uniprot
S1149	Phosphorylation		Uniprot
T1151	Phosphorylation		Uniprot
S1153	Phosphorylation		Uniprot
S1154	Phosphorylation		Uniprot
T1156	Phosphorylation		Uniprot
T1157	Phosphorylation		Uniprot
T1159	Phosphorylation		Uniprot
S1162	Phosphorylation		Uniprot
S1164	Phosphorylation		Uniprot
K1170	Methylation		Uniprot
S1174	Phosphorylation		Uniprot
S1176	Phosphorylation		Uniprot
S1181	Phosphorylation		Uniprot
S1185	Phosphorylation		Uniprot
S1186	Phosphorylation		Uniprot
T1202	Phosphorylation		Uniprot
S1203	Phosphorylation		Uniprot
T1221	Phosphorylation		Uniprot
S1234	Phosphorylation		Uniprot
S1237	Phosphorylation		Uniprot
T1243	Phosphorylation		Uniprot
Y1253	Phosphorylation		Uniprot
S1283	Phosphorylation		Uniprot
Y1320	Phosphorylation		Uniprot

Research Backgrounds

Q9Y4H2_IRS2_HUMAN

Function:

May mediate the control of various cellular processes by insulin.

Subcellular Location:

Cytoplasm>Cytosol.

Subunit Structure:

Interacts with PHIP.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > FoxO signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > AMPK signaling pathway. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Type II diabetes mellitus.

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Human Diseases > Endocrine and metabolic diseases > Non-alcoholic fatty liver disease (NAFLD).

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Aging > Longevity regulating pathway. (View pathway)

· Organismal Systems > Aging > Longevity regulating pathway - multiple species. (View pathway)

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

References

1). Methyl ferulic acid ameliorates alcohol-induced hepatic insulin resistance via miR-378b-mediated activation of PI3K-AKT pathway. BIOMEDICINE & PHARMACOTHERAPY, 2022 (PubMed: 34844105) [IF=7.5]

2). Chronic timed sleep restriction attenuates LepRb-mediated signaling pathways and circadian clock gene expression in the rat hypothalamus. Frontiers in Neuroscience, 2020 (PubMed: 33013300) [IF=4.3]

Application: WB Species: rat Sample:

FIGURE 4 | Sleep restriction induced depletion of circadian clock expression. (A) Western blot analysis. Images are representative of five independent experiments for each group.

3). YiQi YangYin Decoction Attenuates Nonalcoholic Fatty Liver Disease in Type 2 Diabetes Rats. Evidence-based Complementary and Alternative Medicine, 2021 (PubMed: 34621322)

Application: WB Species: Rat Sample: The liver and pancreas tissues

Figure 11 Effect of YQ on insulin secretion-related proteins in pancreas islet. The expressions levels of IRS-2, P-PI3K, PI3K, P-Akt, Akt, and GLUT4 were assessed by western blot analysis. The results are shown as means ± SEM (n = 9–10). ##P < 0.01 compared with the control group; ∗P < 0.05, ∗∗P < 0.01 compared with the model group.

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IRS2 Antibody - #DF7534