Product: TRPM2 Antibody
Catalog: DF7533
Description: Rabbit polyclonal antibody to TRPM2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Chicken
Mol.Wt.: 115kD,171kD; 171kD(Calculated).
Uniprot: O94759
RRID: AB_2841032

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Zebrafish(83%), Bovine(100%), Horse(92%), Sheep(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
TRPM2 Antibody detects endogenous levels of total TRPM2.
RRID:
AB_2841032
Cite Format: Affinity Biosciences Cat# DF7533, RRID:AB_2841032.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EREG 1; EREG1; Estrogen responsive element associated gene 1; Estrogen responsive element associated gene 1 protein; Estrogen-responsive element-associated gene 1 protein; KNP 3; KNP3; Long transient receptor potential channel 2; LTRPC 2; LTrpC-2; LTrpC2; MGC133383; NUDT9H; NUDT9L1; putative Ca2+ channel protein; putative Ca2+ channel protein; Transient receptor potential cation channel subfamily M member 2; Transient receptor potential channel 7; TRPC 7; TrpC7; TRPM 2; Trpm2; TRPM2_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O94759 TRPM2_HUMAN:

Highly expressed in brain and peripheral blood cells, such as neutrophils. Also detected in bone marrow, spleen, heart, liver and lung. Isoform 2 is found in neutrophil granulocytes.

Sequence:
MEPSALRKAGSEQEEGFEGLPRRVTDLGMVSNLRRSNSSLFKSWRLQCPFGNNDKQESLSSWIPENIKKKECVYFVESSKLSDAGKVVCQCGYTHEQHLEEATKPHTFQGTQWDPKKHVQEMPTDAFGDIVFTGLSQKVKKYVRVSQDTPSSVIYHLMTQHWGLDVPNLLISVTGGAKNFNMKPRLKSIFRRGLVKVAQTTGAWIITGGSHTGVMKQVGEAVRDFSLSSSYKEGELITIGVATWGTVHRREGLIHPTGSFPAEYILDEDGQGNLTCLDSNHSHFILVDDGTHGQYGVEIPLRTRLEKFISEQTKERGGVAIKIPIVCVVLEGGPGTLHTIDNATTNGTPCVVVEGSGRVADVIAQVANLPVSDITISLIQQKLSVFFQEMFETFTESRIVEWTKKIQDIVRRRQLLTVFREGKDGQQDVDVAILQALLKASRSQDHFGHENWDHQLKLAVAWNRVDIARSEIFMDEWQWKPSDLHPTMTAALISNKPEFVKLFLENGVQLKEFVTWDTLLYLYENLDPSCLFHSKLQKVLVEDPERPACAPAAPRLQMHHVAQVLRELLGDFTQPLYPRPRHNDRLRLLLPVPHVKLNVQGVSLRSLYKRSSGHVTFTMDPIRDLLIWAIVQNRRELAGIIWAQSQDCIAAALACSKILKELSKEEEDTDSSEEMLALAEEYEHRAIGVFTECYRKDEERAQKLLTRVSEAWGKTTCLQLALEAKDMKFVSHGGIQAFLTKVWWGQLSVDNGLWRVTLCMLAFPLLLTGLISFREKRLQDVGTPAARARAFFTAPVVVFHLNILSYFAFLCLFAYVLMVDFQPVPSWCECAIYLWLFSLVCEEMRQLFYDPDECGLMKKAALYFSDFWNKLDVGAILLFVAGLTCRLIPATLYPGRVILSLDFILFCLRLMHIFTISKTLGPKIIIVKRMMKDVFFFLFLLAVWVVSFGVAKQAILIHNERRVDWLFRGAVYHSYLTIFGQIPGYIDGVNFNPEHCSPNGTDPYKPKCPESDATQQRPAFPEWLTVLLLCLYLLFTNILLLNLLIAMFNYTFQQVQEHTDQIWKFQRHDLIEEYHGRPAAPPPFILLSHLQLFIKRVVLKTPAKRHKQLKNKLEKNEEAALLSWEIYLKENYLQNRQFQQKQRPEQKIEDISNKVDAMVDLLDLDPLKRSGSMEQRLASLEEQVAQTAQALHWIVRTLRASGFSSEADVPTLASQKAAEEPDAEPGGRKKTEEPGDSYHVNARHLLYPNCPVTRFPVPNEKVPWETEFLIYDPPFYTAERKDAAAMDPMGDTLEPLSTIQYNVVDGLRDRRSFHGPYTVQAGLPLNPMGRTGLRGRGSLSCFGPNHTLYPMVTRWRRNEDGAICRKSIKKMLEVLVVKLPLSEHWALPGGSREPGEMLPRKLKRILRQEHWPSFENLLKCGMEVYKGYMDDPRNTDNAWIETVAVSVHFQDQNDVELNRLNSNLHACDSGASIRWQVVDRRIPLYANHKTLLQKAAAEFGAHY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Pig
92
Horse
92
Chicken
92
Zebrafish
83
Dog
0
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O94759 As Substrate

Site PTM Type Enzyme
K8 Ubiquitination
S38 Phosphorylation
S39 Phosphorylation
K55 Ubiquitination
K117 Ubiquitination
Y231 Phosphorylation
K307 Ubiquitination
K314 Ubiquitination
K405 Acetylation
K423 Ubiquitination
K439 Ubiquitination
K596 Ubiquitination
S603 Phosphorylation
T616 Phosphorylation
T618 Phosphorylation
T669 Phosphorylation
S671 Phosphorylation
Y682 Phosphorylation
K703 Ubiquitination
T768 Phosphorylation
Y863 Phosphorylation
S865 Phosphorylation
T884 Phosphorylation
K1104 Acetylation
K1168 Ubiquitination
S1170 Phosphorylation
S1172 Phosphorylation
K1494 Ubiquitination

Research Backgrounds

Function:

Nonselective, voltage-independent cation channel that mediates Na(+) and Ca(2+) influx, leading to increased cytoplasmic Ca(2+) levels. Functions as ligand-gated ion channel. Binding of ADP-ribose to the cytoplasmic Nudix domain causes a conformation change; the channel is primed but still requires Ca(2+) binding to trigger channel opening. Extracellular calcium passes through the channel and increases channel activity. Contributes to Ca(2+) release from intracellular stores in response to ADP-ribose. Plays a role in numerous processes that involve signaling via intracellular Ca(2+) levels (Probable). Besides, mediates the release of lysosomal Zn(2+) stores in response to reactive oxygen species, leading to increased cytosolic Zn(2+) levels. Activated by moderate heat (35 to 40 degrees Celsius). Activated by intracellular ADP-ribose, beta-NAD (NAD(+)) and similar compounds, and by oxidative stress caused by reactive oxygen or nitrogen species. The precise physiological activators are under debate; the true, physiological activators may be ADP-ribose and ADP-ribose-2'-phosphate. Activation by ADP-ribose and beta-NAD is strongly increased by moderate heat (35 to 40 degrees Celsius). Likewise, reactive oxygen species lower the threshold for activation by moderate heat (37 degrees Celsius). Plays a role in mediating behavorial and physiological responses to moderate heat and thereby contributes to body temperature homeostasis. Plays a role in insulin secretion, a process that requires increased cytoplasmic Ca(2+) levels (By similarity). Required for normal IFNG and cytokine secretion and normal innate immune immunity in response to bacterial infection. Required for normal phagocytosis and cytokine release by macrophages exposed to zymosan (in vitro). Plays a role in dendritic cell differentiation and maturation, and in dendritic cell chemotaxis via its role in regulating cytoplasmic Ca(2+) levels (By similarity). Plays a role in the regulation of the reorganization of the actin cytoskeleton and filopodia formation in response to reactive oxygen species via its role in increasing cytoplasmic Ca(2+) and Zn(2+) levels. Confers susceptibility to cell death following oxidative stress.

Lacks cation channel activity. Does not mediate cation transport in response to oxidative stress or ADP-ribose.

Lacks cation channel activity and negatively regulates the channel activity of isoform 1. Negatively regulates susceptibility to cell death in reposponse to oxidative stress.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Perikaryon. Cell projection. Cytoplasmic vesicle. Lysosome.
Note: Detected at the cell membrane and in intracellular vesicles in cortical neurons. Detected on neuronal cell bodies and neurites (By similarity). Detected on the cell membrane in polymorphonuclear neutrophils. Detected on cytoplasmic vesicles and lysosomes in immature bone marrow dendritic cells (By similarity).

Cell membrane>Multi-pass membrane protein.

Cell membrane>Multi-pass membrane protein.

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in brain and peripheral blood cells, such as neutrophils. Also detected in bone marrow, spleen, heart, liver and lung. Isoform 2 is found in neutrophil granulocytes.

Subunit Structure:

Homotetramer. Isoform 1 can interact with isoform 3. This interaction decreases calcium influx through isoform 1 and suppresses susceptibility to oxidative stress-induced cell death.

Family&Domains:

The cytosolic nudix box binds ADP-ribose and is required for channel activation by ADP-ribose (PubMed:15561722, PubMed:16601673, PubMed:30467180).

Belongs to the transient receptor (TC 1.A.4) family. LTrpC subfamily. TRPM2 sub-subfamily.

Research Fields

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Oxytocin signaling pathway.

References

1). Knocking down TRPM2 expression reduces cell injury and NLRP3 inflammasome activation in PC12 cells subjected to oxygen-glucose deprivation. Neural Regeneration Research, 2020 (PubMed: 32394974) [IF=6.1]

Application: WB    Species:    Sample: PC12 cells

Figure 1| Verification of TRMP2 knockdown efficiency. (B) TRPM2 protein expression. TRPM2 expression was normalized to β-actin expression based on grayscale analysis. Data are expressed as the mean ± standard deviation (SD)(n = 6; one-way analysis of variance followed by Student-Newman-Keuls post hoc test). *P < 0.05, vs. shRNA-NC group. NC: Normal control; OGD:oxygen-glucose deprivation; PC12: pheochromocytoma; TRPM2: transient receptor potential melastatin 2.

2). Down-regulation of TRPM2 attenuates hepatic ischemia/reperfusion injury through activation of autophagy and inhibition of NLRP3 inflammasome pathway. International Immunopharmacology, 2022 (PubMed: 35021129) [IF=5.6]

Application: IF/ICC    Species:    Sample: hepatocytes

Fig. 1.|Expression of TRPM2 (B) was up-regulated after glucose and oxygen deprivation, and reached the maximum value after 12 h. Scale bar, 50 µm. Data presented as means ± SD and compared by one-way ANOVA and SNK method. *P < 0.05, **P < 0.01, ***P < 0.001.

3). Puerarin Alleviates Vascular Cognitive Impairment in Vascular Dementia Rats. Frontiers in Behavioral Neuroscience, 2021 (PubMed: 34720898) [IF=3.0]

Application: WB    Species: Rat    Sample: hippocampus

FIGURE 7 Expression and co-localization of NR2A and transient receptor potential melastatin-related 2 (TRPM2) in the rat hippocampus. (A) Protein amounts of TRPM2 in the hippocampal dorsal CA1 region of rats. (B) Protein amounts of NR2A in the hippocampal dorsal CA1 region of rats. (C) Effects of Pur on NR2A and TRPM2 co-localization in the hippocampal dorsal CA1 region, examined by immunofluorescence double staining. Representative micrographs are shown at ×200. Results were mean ± SEM (n = 6). **P < 0.01 vs. Sham; ##P < 0.01 vs. BCCAO; $$P < 0.01 vs. BCCAO + Pur (150 mg/kg/day).

Application: IF/ICC    Species: Rat    Sample: hippocampus

FIGURE 7 Expression and co-localization of NR2A and transient receptor potential melastatin-related 2 (TRPM2) in the rat hippocampus. (A) Protein amounts of TRPM2 in the hippocampal dorsal CA1 region of rats. (B) Protein amounts of NR2A in the hippocampal dorsal CA1 region of rats. (C) Effects of Pur on NR2A and TRPM2 co-localization in the hippocampal dorsal CA1 region, examined by immunofluorescence double staining. Representative micrographs are shown at ×200. Results were mean ± SEM (n = 6). **P < 0.01 vs. Sham; ##P < 0.01 vs. BCCAO; $$P < 0.01 vs. BCCAO + Pur (150 mg/kg/day).

4). TRPM2 mediates CaMKⅡ-Beclin-1 signaling in early cortical injury after induced subarachnoid hemorrhage in mice. JOURNAL OF CHEMICAL NEUROANATOMY, 2022 (PubMed: 35988814) [IF=2.8]

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