Claspin Antibody - #DF7525

(2)  
Product: Claspin Antibody
Catalog: DF7525
Description: Rabbit polyclonal antibody to Claspin
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 150 kDa; 151kD(Calculated).
Uniprot: Q9HAW4
RRID: AB_2841024

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Claspin Antibody detects endogenous levels of total Claspin.
RRID:
AB_2841024
Cite Format: Affinity Biosciences Cat# DF7525, RRID:AB_2841024.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Claspin; claspin homolog (Xenopus laevis); Claspin homolog; CLSPN; CLSPN protein; CLSPN_HUMAN; hClaspin; Hu Claspin;

Immunogens

Immunogen:
Uniprot:

Q9HAW4(CLSPN_HUMAN) >>Visit HPA database.

Gene(ID):
CLSPN(63967)
Sequence:
MTGEVGSEVHLEINDPNVISQEEADSPSDSGQGSYETIGPLSEGDSDEEIFVSKKLKNRKVLQDSDSETEDTNASPEKTTYDSAEEENKENLYAGKNTKIKRIYKTVADSDESYMEKSLYQENLEAQVKPCLELSLQSGNSTDFTTDRKSSKKHIHDKEGTAGKAKVKSKRRLEKEERKMEKIRQLKKKETKNQEDDVEQPFNDSGCLLVDKDLFETGLEDENNSPLEDEESLESIRAAVKNKVKKHKKKEPSLESGVHSFEEGSELSKGTTRKERKAARLSKEALKQLHSETQRLIRESALNLPYHMPENKTIHDFFKRKPRPTCHGNAMALLKSSKYQSSHHKEIIDTANTTEMNSDHHSKGSEQTTGAENEVETNALPVVSKETQIITGSDESCRKDLVKNEELEIQEKQKQSDIRPSPGDSSVLQQESNFLGNNHSEECQVGGLVAFEPHALEGEGPQNPEETDEKVEEPEQQNKSSAVGPPEKVRRFTLDRLKQLGVDVSIKPRLGADEDSFVILEPETNRELEALKQRFWKHANPAAKPRAGQTVNVNVIVKDMGTDGKEELKADVVPVTLAPKKLDGASHTKPGEKLQVLKAKLQEAMKLRRFEERQKRQALFKLDNEDGFEEEEEEEEEMTDESEEDGEEKVEKEEKEEELEEEEEKEEEEEEEGNQETAEFLLSSEEIETKDEKEMDKENNDGSSEIGKAVGFLSVPKSLSSDSTLLLFKDSSSKMGYFPTEEKSETDENSGKQPSKLDEDDSCSLLTKESSHNSSFELIGSTIPSYQPCNRQTGRGTSFFPTAGGFRSPSPGLFRASLVSSASKSSGKLSEPSLPIEDSQDLYNASPEPKTLFLGAGDFQFCLEDDTQSQLLDADGFLNVRNHRNQYQALKPRLPLASMDENAMDANMDELLDLCTGKFTSQAEKHLPRKSDKKENMEELLNLCSGKFTSQDASTPASSELNKQEKESSMGDPMEEALALCSGSFPTDKEEEDEEEEFGDFRLVSNDNEFDSDEDEHSDSGNDLALEDHEDDDEEELLKRSEKLKRQMRLRKYLEDEAEVSGSDVGSEDEYDGEEIDEYEEDVIDEVLPSDEELQSQIKKIHMKTMLDDDKRQLRLYQERYLADGDLHSDGPGRMRKFRWKNIDDASQMDLFHRDSDDDQTEEQLDESEARWRKERIEREQWLRDMAQQGKITAEEEEEIGEDSQFMILAKKVTAKALQKNASRPMVIQESKSLLRNPFEAIRPGSAQQVKTGSLLNQPKAVLQKLAALSDHNPSAPRNSRNFVFHTLSPVKAEAAKESSKSQVKKRGPSFMTSPSPKHLKTDDSTSGLTRSIFKYLES

PTMs - Q9HAW4 As Substrate

Site PTM Type Enzyme
Ubiquitination
S30 Phosphorylation P53350 (PLK1)
S34 Phosphorylation
K60 Ubiquitination
S65 Phosphorylation
S67 Phosphorylation O00311 (CDC7)
T69 Phosphorylation
S75 Phosphorylation
Y81 Phosphorylation
S83 Phosphorylation
Y93 Phosphorylation
K96 Ubiquitination
K105 Ubiquitination
S110 Phosphorylation O00311 (CDC7)
Y120 Phosphorylation
K187 Acetylation
K188 Acetylation
K192 Acetylation
S205 Phosphorylation
S225 Phosphorylation
S232 Phosphorylation
S260 Phosphorylation O00311 (CDC7)
S265 Phosphorylation O00311 (CDC7)
S282 Phosphorylation
K283 Ubiquitination
K287 Ubiquitination
Y306 Phosphorylation
K319 Ubiquitination
K403 Sumoylation
T493 Phosphorylation
K507 Ubiquitination
S516 Phosphorylation
K532 Ubiquitination
K606 Acetylation
T639 Phosphorylation
S642 Phosphorylation
S703 Phosphorylation
S704 Phosphorylation O00311 (CDC7)
K708 Ubiquitination
S714 Phosphorylation
S718 Phosphorylation
S720 Phosphorylation
S721 Phosphorylation O00311 (CDC7)
S723 Phosphorylation
T724 Phosphorylation
S731 Phosphorylation
K734 Ubiquitination
S744 Phosphorylation O00311 (CDC7)
S750 Phosphorylation
K756 Acetylation
S762 Phosphorylation
S764 Phosphorylation
S774 Phosphorylation
S775 Phosphorylation
T802 Phosphorylation
S808 Phosphorylation
S810 Phosphorylation
S823 Phosphorylation
K824 Ubiquitination
K828 Ubiquitination
S833 Phosphorylation
S839 Phosphorylation
Y843 Phosphorylation
S846 Phosphorylation
Y887 Phosphorylation
K891 Acetylation
K891 Ubiquitination
T916 Phosphorylation O14757 (CHEK1)
S921 Phosphorylation
K925 Acetylation
K925 Ubiquitination
S945 Phosphorylation
K947 Ubiquitination
T949 Phosphorylation
S950 Phosphorylation
S954 Phosphorylation
T955 Phosphorylation
S958 Phosphorylation
S959 Phosphorylation
K963 Ubiquitination
S982 Phosphorylation
S984 Phosphorylation Q9BTU6 (PI4K2A)
T987 Phosphorylation
S1012 Phosphorylation
S1018 Phosphorylation
S1020 Phosphorylation
S1129 Phosphorylation O00311 (CDC7)
S1147 Phosphorylation O00311 (CDC7)
S1156 Phosphorylation
T1161 Phosphorylation
S1204 Phosphorylation
S1223 Phosphorylation
K1265 Ubiquitination
T1287 Phosphorylation
S1289 Phosphorylation
K1292 Sumoylation
S1310 Phosphorylation
S1314 Phosphorylation
S1316 Phosphorylation
K1321 Sumoylation
K1321 Ubiquitination
T1322 Phosphorylation

Research Backgrounds

Function:

Required for checkpoint mediated cell cycle arrest in response to inhibition of DNA replication or to DNA damage induced by both ionizing and UV irradiation. Adapter protein which binds to BRCA1 and the checkpoint kinase CHEK1 and facilitates the ATR-dependent phosphorylation of both proteins. Can also bind specifically to branched DNA structures and may associate with S-phase chromatin following formation of the pre-replication complex (pre-RC). This may indicate a role for this protein as a sensor which monitors the integrity of DNA replication forks.

PTMs:

Phosphorylated. Undergoes ATR-dependent phosphorylation by CHEK1 during activation of DNA replication or damage checkpoints. Phosphorylation by CSNK1G1/CK1 promotes CHEK1 binding.

Ubiquitinated by the anaphase promoting complex/cyclosome (APC/C) during G1 phase, leading to its degradation by the proteasome. Ubiquitination is mediated via its interaction with FZR1/CDH1. Following DNA damage, it is deubiquitinated by USP28 in G2 phase, preventing its degradation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts (phosphorylation-dependent) with CHEK1; regulates CLSPN function in checkpoint for DNA damage and replication. Interacts with ATR and RAD9A and these interactions are slightly reduced during checkpoint activation. Interacts with BRCA1 and this interaction increases during checkpoint activation. Interacts with TIMELESS.

Family&Domains:

The C-terminus of the protein contains 3 potential CHEK1-binding motifs (CKB motifs). Potential phosphorylation sites within CKB motif 1 and CKB motif 2 are required for interaction with CHEK1.

Belongs to the claspin family.

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