Gli1 Antibody | Affinity Biosciences

Product:	Gli1 Antibody
Catalog:	DF7523
Description:	Rabbit polyclonal antibody to Gli1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	118 kDa; 118kD(Calculated).
Uniprot:	P08151
RRID:	AB_2841022

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Secondary Antibodies
Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

Gli1 Antibody detects endogenous levels of total Gli1.

RRID:

AB_2841022
Cite Format: Affinity Biosciences Cat# DF7523, RRID:AB_2841022.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Gli 1; GLI; GLI family zinc finger 1; GLI Kruppel family member 1; gli1; GLI1_HUMAN; Glioma associated oncogene 1; Glioma associated oncogene homolog 1 (zinc finger protein); Glioma associated oncogene homolog; Glioma-associated oncogene; Oncogene GLI; Zfp 5; Zfp5; Zinc finger protein GLI 1; Zinc finger protein GLI1;

Immunogens

Immunogen:

Uniprot:

P08151(GLI1_HUMAN) >>Visit HPA database.

Gene(ID):

GLI1(2735)

Expression:

P08151 GLI1_HUMAN:

Detected in testis (at protein level) (PubMed:2105456). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract (PubMed:19878745). Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues (PubMed:19706761).

Sequence:

P08151 GLI1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MFNSMTPPPISSYGEPCCLRPLPSQGAPSVGTEGLSGPPFCHQANLMSGPHSYGPARETNSCTEGPLFSSPRSAVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPAQMNHQKGPSPSFGVQPCGPHDSARGGMIPHPQSRGPFPTCQLKSELDMLVGKCREEPLEGDMSSPNSTGIQDPLLGMLDGREDLEREEKREPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEFVCHWGGCSRELRPFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEHEGCSKAFSNASDRAKHQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAPSISTVEPKREREGGPIREESRLTVPEGAMKPQPSPGAQSSCSSDHSPAGSAANTDSGVEMTGNAGGSTEDLSSLDEGPCIAGTGLSTLRRLENLRLDQLHQLRPIGTRGLKLPSLSHTGTTVSRRVGPPVSLERRSSSSSSISSAYTVSRRSSLASPFPPGSPPENGASSLPGLMPAQHYLLRARYASARGGGTSPTAASSLDRIGGLPMPPWRSRAEYPGYNPNAGVTRRASDPAQAADRPAPARVQRFKSLGCVHTPPTVAGGGQNFDPYLPTSVYSPQPPSITENAAMDARGLQEEPEVGTSMVGSGLNPYMDFPPTDTLGYGGPEGAAAEPYGARGPGSLPLGPGPPTNYGPNPCPQQASYPDPTQETWGEFPSHSGLYPGPKALGGTYSQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSEGPPHPQPLFSHYPQPSPPQYLQSGPYTQPPPDYLPSEPRPCLDFDSPTHSTGQLKAQLVCNYVQSQQELLWEGGGREDAPAQEPSYQSPKFLGGSQVSPSRAKAPVNTYGPGFGPNLPNHKSGSYPTPSPCHENFVVGANRASHRAAAPPRLLPPLPTCYGPLKVGGTNPSCGHPEVGRLGGGPALYPPPEGQVCNPLDSLDLDNTQLDFVAILDEPQGLSPPPSHDQRGSSGHTPPPSGPPNMAVGNMSVLLRSLPGETEFLNSSA

PTMs - P08151 As Substrate

P08151

Site	PTM Type	Enzyme	Source
	Phosphorylation		Uniprot
	Ubiquitination		Uniprot
T63	Phosphorylation		Uniprot
S69	Phosphorylation		Uniprot
S84	Phosphorylation	P23443 (RPS6KB1)	Uniprot
S102	Phosphorylation	P54646 (PRKAA2)	Uniprot
S130	Phosphorylation		Uniprot
R161	Methylation		Uniprot
S201	Phosphorylation		Uniprot
S204	Phosphorylation		Uniprot
T205	Phosphorylation		Uniprot
S243	Phosphorylation	P41743 (PRKCI)	Uniprot
T304	Phosphorylation	P41743 (PRKCI)	Uniprot
K319	Ubiquitination		Uniprot
K365	Acetylation		Uniprot
K371	Acetylation		Uniprot
T374	Phosphorylation	P17612 (PRKACA)	Uniprot
S408	Phosphorylation	P54646 (PRKAA2)	Uniprot
K518	Acetylation		Uniprot
S521	Phosphorylation		Uniprot
T527	Phosphorylation		Uniprot
S538	Phosphorylation		Uniprot
S544	Phosphorylation	P17612 (PRKACA)	Uniprot
S545	Phosphorylation		Uniprot
S560	Phosphorylation	P17612 (PRKACA)	Uniprot
R597	Methylation		Uniprot
S602	Phosphorylation		Uniprot
S622	Phosphorylation		Uniprot
S640	Phosphorylation	P17612 (PRKACA)	Uniprot
S934	Phosphorylation		Uniprot
S937	Phosphorylation		Uniprot
S939	Phosphorylation		Uniprot
S968	Phosphorylation		Uniprot
S1071	Phosphorylation		Uniprot
T1074	Phosphorylation	P54646 (PRKAA2)	Uniprot
S1078	Phosphorylation		Uniprot

Research Backgrounds

P08151_GLI1_HUMAN

Function:

Acts as a transcriptional activator. Binds to the DNA consensus sequence 5'-GACCACCCA-3'. Regulates the transcription of specific genes during normal development. Plays a role in craniofacial development and digital development, as well as development of the central nervous system and gastrointestinal tract. Mediates SHH signaling. Plays a role in cell proliferation and differentiation via its role in SHH signaling.

Acts as a transcriptional activator, but activates a different set of genes than isoform 1. Activates expression of CD24, unlike isoform 1. Mediates SHH signaling. Promotes cancer cell migration.

PTMs:

Phosphorylated in vitro by ULK3.

Acetylation at Lys-518 down-regulates transcriptional activity. Deacetylated by HDAC1.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Tethered in the cytoplasm by binding to SUFU (PubMed:10806483). Activation and translocation to the nucleus is promoted by interaction with STK36 (PubMed:10806483). Phosphorylation by ULK3 may promote nuclear localization (PubMed:19878745). Translocation to the nucleus is promoted by interaction with ZIC1 (PubMed:11238441).

Cytoplasm. Nucleus.

Tissue Specificity:

Detected in testis (at protein level). Testis, myometrium and fallopian tube. Also expressed in the brain with highest expression in the cerebellum, optic nerve and olfactory tract. Isoform 1 is detected in brain, spleen, pancreas, liver, kidney and placenta; isoform 2 is not detectable in these tissues.

Subunit Structure:

Interacts with KIF7 (By similarity). Interacts with STK36. Interacts with ZIC1; the interaction enhances transcription activation. Interacts with SUFU; this inhibits transcriptional activation by GLI1.

Family&Domains:

Belongs to the GLI C2H2-type zinc-finger protein family.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Specific types > Basal cell carcinoma. (View pathway)

References

1). Inoscavin A, a pyrone compound isolated from a Sanghuangporus vaninii extract, inhibits colon cancer cell growth and induces cell apoptosis via the hedgehog signaling pathway. Phytomedicine, 2022 (PubMed: 35026508) [IF=7.9]

2). Hedgehog pathway inhibition causes primary follicle atresia and decreases female germline stem cell proliferation capacity or stemness. Stem Cell Research & Therapy, 2019 (PubMed: 31277696) [IF=7.5]

Application: IHC Species: mouse Sample: ovarian

Fig. 1 | Hh signaling pathway activity decreases with ovarian aging. a mRNA expression levels of Gli1, Ptch1, and Cyclin D1 in three mice of reproductive age. b, c Protein expression levels.d IHC detection of key members of the Hh pathway, Gli1, Ptch1, and Cyclin D1, in the OSE. Bar is 10 μm. *p < 0.05 and **p < 0.01

Application: IF/ICC Species: mouse Sample: ovarian

Fig. 2| Ovarian aging has a similar trend as the decrease in Hh activity. a mRNA levels of Mvh decrease gradually along with ovarian aging. bOct4 mRNA levels have the same trend as Mvh levels. c, d Protein levels of Mvh and Oct4 at three reproductive age points. e, f Dual-IF showed the coexpression of Mvh and Gli1, Oct4, and Gli1 in OSE. Bar is 20 μm. *p < 0.05 and **p < 0.01

Application: WB Species: mouse Sample: ovarian

Fig. 3| Pathological ovaries exhibited a decline in Hh pathway activity. a HE staining of a normal ovary and POF ovary. Bar is 200 μm. b The ratio of atretic follicles of normal and POF ovaries. c IHC shows that the Mvh-positive follicles and FGSCs dramatically disappeared in the POF model;green arrows are FGSCs, and black arrow represents primordial follicles, the scale is 100 μm. d–f mRNA and protein expression levels of Mvh, Oct4, Gli1,Ptch1, and Cyclin D1. *p < 0.05 and **p < 0.01

Application: WB Species: mouse Sample: ovaries

Fig. |1 Hh signaling pathway activity decreases with ovarian aging. a mRNA expression levels of Gli1, Ptch1, and Cyclin D1 in three mice of reproductive age. b, c Protein expression levels.

Application: IHC Species: mouse Sample: ovaries

Fig. |1 Hh signaling pathway activity decreases with ovarian aging. a mRNA expression levels of Gli1, Ptch1, and Cyclin D1 in three mice of reproductive age. b, c Protein expression levels.d IHC detection of key members of the Hh pathway, Gli1, Ptch1, and Cyclin D1, in the OSE. Bar is 10 μm. *p < 0.05 and **p < 0.01

Application: IF/ICC Species: mouse Sample: ovaries

Fig. 2 |Ovarian aging has a similar trend as the decrease in Hh activity. a mRNA levels of Mvh decrease gradually along with ovarian aging. bOct4 mRNA levels have the same trend as Mvh levels. c, d Protein levels of Mvh and Oct4 at three reproductive age points. e, f Dual-IF showed the coexpression of Mvh and Gli1, Oct4, and Gli1 in OSE. Bar is 20 μm. *p < 0.05 and **p < 0.01

3). Resveratrol Plays a Protective Role against Premature Ovarian Failure and Prompts Female Germline Stem Cell Survival. International Journal of Molecular Sciences, 2019 (PubMed: 31340581) [IF=5.6]

4). Effects of metformin on Sonic hedgehog subgroup medulloblastoma progression: In vitro and in vivo studies. Frontiers in Pharmacology, 2022 (PubMed: 36278239) [IF=5.6]

5). Gankyrin activates the hedgehog signalling to drive metastasis in osteosarcoma. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2021 (PubMed: 34089292) [IF=5.3]

Application: WB Species: Human Sample: U2OS cells

FIGURE 3 Gankyrin promotes invasion, migration and regulates the expression of some stemness factors by up‐regulating Gli1. A, Gankyrin knockdown in U2OS cells reduced the mRNA expression of Gli1 and Ptch1 but not of β‐catenin and AXIN2, as shown by qRT‐PCR analysis. ns, P ≥ .05; *P < .05. B, Overexpression of gankyrin in MG63 cells increased the mRNA expression of Gli1 and Ptch1 but not of β‐catenin and AXIN2. ns, P ≥ .05; **P < .01. C, Gankyrin depletion in U2OS cells diminished the protein levels of Gli1 and PTCH1, as detected by western blot analysis. D, Ectopic expression of gankyrin in MG63 cells induced the protein levels of Gli1 and PTCH1. E, The up‐regulation of stem cell markers, including CD133, OCT4, and Nanog, following gankyrin overexpression was attenuated by Gli1 shRNA in U2OS cells, as detected by Western blot analysis. F, The increase in cell migration with gankyrin overexpression was attenuated by Gli1 shRNA in U2OS cells, as shown by wound healing assays. *P < .05; **P < .01. G, The increase in cell migration with gankyrin overexpression was attenuated by Gli1 shRNA in U2OS cells, as shown by Transwell invasion assays. *P < .05

6). Hedgehog Signaling Pathway Regulates the Proliferation and Differentiation of Rat Meibomian Gland Epithelial Cells. Investigative Ophthalmology & Visual Science, 2021 (PubMed: 33616621) [IF=4.4]

Application: IF/ICC Species: Rat Sample: epithelial cells

Figure 1. Expression of Hedgehog receptor and downstream molecules in rat meibomian glands. (A) At P3, Smo was broadly expressed in almost all epithelial cells. By 6 weeks, Smo was expressed in all acini, and by 12 weeks, the expression of Smo in acini were generally low. (B) Gli1 was expressed in the cytoplasm of meibomian glands at 3 days, 6 weeks, and 12 weeks old. (C) Gli2 was highly expressed in the cytoplasm at 3 days old, but by 6 weeks and 12 weeks, the expression was almost absent. (D) Gli3 was hardly expressed at 3 days, 6 weeks, and 12 weeks. Du, duct; Ac, acinus.

7). (‑)‑Epigallocatechin‑3‑gallate inhibits bladder cancer stem cells via suppression of sonic hedgehog pathway. ONCOLOGY REPORTS, 2019 (PubMed: 31180522) [IF=4.2]

Application: WB Species: human Sample: Bladder CSCs

Figure 3.| - EGCG induces apoptosis and reduces the expression of cell proliferation-related proteins on bladder CSCs. Bladder CSCs were treated with different concentrations of EGCG for 7 days. (D) The expression levels of Shh signaling pathway-related proteins including Shh, Smo, Gli1 and Gli2 were detected by western blot analysis. Data are expressed as mean ± standard deviation. *P<0.05, **P<0.01 vs. the EGCG-0 µm group. EGCG, (−)-epigallocatechin-3-gallate; CSCs, cancer stem cells; PCNA, proliferating cell nuclear antigen; Shh, sonic hedgehog protein; Smo, smoothened homolog; Gli, zinc finger protein GLI.

8). Curcumin mitigates the epithelial-to-mesenchymal transition in biliary epithelial cells through upregulating CD109 expression. DRUG DEVELOPMENT RESEARCH, 2019 (PubMed: 31403228) [IF=3.8]

Application: WB Species: human Sample: HIBECs

FIGURE 5 |Curcumin suppresses the TGF-β1-activated Hedgehog signaling in HIBECs. (a) Quantitative analysis of Shh, Gli1, Smo, Ptch-1, and Sufu protein expression.

9). IRE1α inhibits osteogenic differentiation of mouse embryonic fibroblasts by limiting Shh signaling. Oral diseases, 2024 (PubMed: 38438324) [IF=3.8]

10). Dehydroepiandrosterone alleviates E. Coli O157:H7-induced inflammation by preventing the activation of p38 MAPK and NF-κB pathways in mice peritoneal macrophages. MOLECULAR IMMUNOLOGY, 2019 (PubMed: 31351412) [IF=3.6]

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Gli1 Antibody - #DF7523