Rad21 Antibody - #DF7520

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Product: Rad21 Antibody
Catalog: DF7520
Description: Rabbit polyclonal antibody to Rad21
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 130KD; 72kD(Calculated).
Uniprot: O60216
RRID: AB_2841019

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
Rad21 Antibody detects endogenous levels of total Rad21.
RRID:
AB_2841019
Cite Format: Affinity Biosciences Cat# DF7520, RRID:AB_2841019.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CDLS4; Double-strand-break repair protein rad21 homolog; hHR21; HR21; HRAD21; KIAA0078; MCD1; Nuclear matrix protein 1; NXP-1; NXP1; Protein involved in DNA double-strand break repair; RAD21; RAD21 homolog (S. pombe); RAD21 homolog; RAD21_HUMAN; Scc1; SCC1 homolog;

Immunogens

Immunogen:
Uniprot:

O60216(RAD21_HUMAN) >>Visit HPA database.

Gene(ID):
RAD21(5885)
Expression:
O60216 RAD21_HUMAN:

Expressed in the gut (at protein level).

Sequence:
MFYAHFVLSKRGPLAKIWLAAHWDKKLTKAHVFECNLESSVESIISPKVKMALRTSGHLLLGVVRIYHRKAKYLLADCNEAFIKIKMAFRPGVVDLPEENREAAYNAITLPEEFHDFDQPLPDLDDIDVAQQFSLNQSRVEEITMREEVGNISILQENDFGDFGMDDREIMREGSAFEDDDMLVSTTTSNLLLESEQSTSNLNEKINHLEYEDQYKDDNFGEGNDGGILDDKLISNNDGGIFDDPPALSEAGVMLPEQPAHDDMDEDDNVSMGGPDSPDSVDPVEPMPTMTDQTTLVPNEEEAFALEPIDITVKETKAKRKRKLIVDSVKELDSKTIRAQLSDYSDIVTTLDLAPPTKKLMMWKETGGVEKLFSLPAQPLWNNRLLKLFTRCLTPLVPEDLRKRRKGGEADNLDEFLKEFENPEVPREDQQQQHQQRDVIDEPIIEEPSRLQESVMEASRTNIDESAMPPPPPQGVKRKAGQIDPEPVMPPQQVEQMEIPPVELPPEEPPNICQLIPELELLPEKEKEKEKEKEDDEEEEDEDASGGDQDQEERRWNKRTQQMLHGLQRALAKTGAESISLLELCRNTNRKQAAAKFYSFLVLKKQQAIELTQEEPYSDIIATPGPRFHII

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60216 As Substrate

Site PTM Type Enzyme
Sumoylation
S9 Phosphorylation
K29 Ubiquitination
S40 Phosphorylation
S46 Phosphorylation
K48 Ubiquitination
K50 Ubiquitination
T55 Phosphorylation
S56 Phosphorylation
Y67 Phosphorylation
K72 Ubiquitination
K84 Ubiquitination
K86 Ubiquitination
S134 Phosphorylation
S138 Phosphorylation
T144 Phosphorylation
S153 Phosphorylation
S175 Phosphorylation
S185 Phosphorylation
T186 Phosphorylation
T187 Phosphorylation
T188 Phosphorylation
S189 Phosphorylation
S200 Phosphorylation
Y211 Phosphorylation
Y215 Phosphorylation
K216 Ubiquitination
S249 Phosphorylation
S277 Phosphorylation
S280 Phosphorylation
T289 Phosphorylation
T312 Phosphorylation
K323 Ubiquitination
K330 Ubiquitination
K335 Ubiquitination
S342 Phosphorylation
Y344 Phosphorylation
S345 Phosphorylation
T350 Phosphorylation
K358 Ubiquitination
K359 Ubiquitination
K364 Ubiquitination
T366 Phosphorylation
K371 Ubiquitination
S374 Phosphorylation
K387 Ubiquitination
T394 Phosphorylation
K406 Ubiquitination
K418 Ubiquitination
S449 Phosphorylation
S454 Phosphorylation
S459 Phosphorylation
S466 Phosphorylation
K477 Ubiquitination
S545 Phosphorylation
K573 Ubiquitination
K596 Ubiquitination
Y598 Phosphorylation
K605 Ubiquitination
S618 Phosphorylation
T623 Phosphorylation

Research Backgrounds

Function:

As a member of the cohesin complex, involved in sister chromatid cohesion from the time of DNA replication in S phase to their segregation in mitosis, a function that is essential for proper chromosome segregation, post-replicative DNA repair, and the prevention of inappropriate recombination between repetitive regions. The cohesin complex may also play a role in spindle pole assembly during mitosis. In interphase, cohesins may function in the control of gene expression by binding to numerous sites within the genome (By similarity). May control RUNX1 gene expression (Probable). Binds to and represses APOB gene promoter. May play a role in embryonic gut development, possibly through the regulation of enteric neuron development (By similarity).

May promote apoptosis.

PTMs:

Cleaved by separase/ESPL1 at the onset of anaphase; this cleavage is required for sister chromatid separation and cytokinesis. Cleaved by caspase-3/CASP3 or caspase-7/CASP7 at the beginning of apoptosis.

Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK1.

Subcellular Location:

Nucleus. Nucleus matrix. Chromosome. Chromosome>Centromere. Cytoplasm>Cytoskeleton>Spindle pole.
Note: Associates with chromatin (PubMed:11590136, PubMed:11073952). Before prophase, scattered along chromosome arms (PubMed:11073952). During prophase and prometaphase, most cohesins dissociate from the arms of condensing chromosome, possibly through PLK1-mediated phosphorylation (PubMed:11931760). A small amount of cohesin remains in centromeric regions and is removed from chromosomes only at the onset of anaphase. At anaphase, cleavage by separase/ESPL1 leads to the dissociation of cohesin from chromosomes and chromosome separation (PubMed:11073952, PubMed:11509732).

Cytoplasm>Cytosol. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the gut (at protein level).

Subunit Structure:

Component of the cohesin complex, which consists of an SMC1A/B and SMC3 heterodimer core and 2 non-Smc subunits RAD21 and STAG1/SA1, STAG2/SA2 or STAG3/SA3. The cohesin complex interacts with NUMA1. The cohesin complex also interacts with CDCA5, PDS5A and PDS5B; this interaction might regulate the ability of the cohesin complex to mediate sister chromatid cohesion. The interaction with PDS5B is direct and is stimulated by STAG1/SA1. The cohesin complex interacts with the cohesin loading complex subunits NIPBL/Scc2 (via HEAT repeats) and MAU2/Scc4. The cohesin complex interacts with DDX11/ChIR1. Directly interacts with WAPL; this interaction is stimulated by STAG1/SA1.

Family&Domains:

The C-terminal part associates with the head of SMC1A, while the N-terminal part binds to the head of SMC3.

Belongs to the rad21 family.

Research Fields

· Cellular Processes > Cell growth and death > Cell cycle.   (View pathway)

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