LATS1/2 Antibody - #DF7517

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Product: LATS1/2 Antibody
Catalog: DF7517
Description: Rabbit polyclonal antibody to LATS1/2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 160 kDa; 127kD,120kD(Calculated).
Uniprot: O95835 | Q9NRM7
RRID: AB_2841016

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(91%), Bovine(82%), Horse(91%), Sheep(82%), Rabbit(91%)
Clonality:
Polyclonal
Specificity:
LATS1/2 Antibody detects endogenous levels of total LATS1/2.
RRID:
AB_2841016
Cite Format: Affinity Biosciences Cat# DF7517, RRID:AB_2841016.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

h-warts; Large tumor suppressor homolog 1; LATS large tumor suppressor homolog 1; LATS1; LATS1_HUMAN; Serine threonine protein kinase LATS1; Serine/threonine-protein kinase LATS1; WARTS; WARTS protein kinase; wts;

Immunogens

Immunogen:
Uniprot:

O95835(LATS1_HUMAN) >>Visit HPA database.

Q9NRM7(LATS2_HUMAN) >>Visit HPA database.

Gene(ID):
LATS1(9113)
Expression:
O95835 LATS1_HUMAN:

Expressed in all adult tissues examined except for lung and kidney.

Q9NRM7 LATS2_HUMAN:

Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined.

Sequence:
MKRSEKPEGYRQMRPKTFPASNYTVSSRQMLQEIRESLRNLSKPSDAAKAEHNMSKMSTEDPRQVRNPPKFGTHHKALQEIRNSLLPFANETNSSRSTSEVNPQMLQDLQAAGFDEDMVIQALQKTNNRSIEAAIEFISKMSYQDPRREQMAAAAARPINASMKPGNVQQSVNRKQSWKGSKESLVPQRHGPPLGESVAYHSESPNSQTDVGRPLSGSGISAFVQAHPSNGQRVNPPPPPQVRSVTPPPPPRGQTPPPRGTTPPPPSWEPNSQTKRYSGNMEYVISRISPVPPGAWQEGYPPPPLNTSPMNPPNQGQRGISSVPVGRQPIIMQSSSKFNFPSGRPGMQNGTGQTDFMIHQNVVPAGTVNRQPPPPYPLTAANGQSPSALQTGGSAAPSSYTNGSIPQSMMVPNRNSHNMELYNISVPGLQTNWPQSSSAPAQSSPSSGHEIPTWQPNIPVRSNSFNNPLGNRASHSANSQPSATTVTAITPAPIQQPVKSMRVLKPELQTALAPTHPSWIPQPIQTVQPSPFPEGTASNVTVMPPVAEAPNYQGPPPPYPKHLLHQNPSVPPYESISKPSKEDQPSLPKEDESEKSYENVDSGDKEKKQITTSPITVRKNKKDEERRESRIQSYSPQAFKFFMEQHVENVLKSHQQRLHRKKQLENEMMRVGLSQDAQDQMRKMLCQKESNYIRLKRAKMDKSMFVKIKTLGIGAFGEVCLARKVDTKALYATKTLRKKDVLLRNQVAHVKAERDILAEADNEWVVRLYYSFQDKDNLYFVMDYIPGGDMMSLLIRMGIFPESLARFYIAELTCAVESVHKMGFIHRDIKPDNILIDRDGHIKLTDFGLCTGFRWTHDSKYYQSGDHPRQDSMDFSNEWGDPSSCRCGDRLKPLERRAARQHQRCLAHSLVGTPNYIAPEVLLRTGYTQLCDWWSVGVILFEMLVGQPPFLAQTPLETQMKVINWQTSLHIPPQAKLSPEASDLIIKLCRGPEDRLGKNGADEIKAHPFFKTIDFSSDLRQQSASYIPKITHPTDTSNFDPVDPDKLWSDDNEEENVNDTLNGWYKNGKHPEHAFYEFTFRRFFDDNGYPYNYPKPIEYEYINSQGSEQQSDEDDQNTGSEIKNRDLVYV

MRPKTFPATTYSGNSRQRLQEIREGLKQPSKSSVQGLPAGPNSDTSLDAKVLGSKDATRQQQQMRATPKFGPYQKALREIRYSLLPFANESGTSAAAEVNRQMLQELVNAGCDQEMAGRALKQTGSRSIEAALEYISKMGYLDPRNEQIVRVIKQTSPGKGLMPTPVTRRPSFEGTGDSFASYHQLSGTPYEGPSFGADGPTALEEMPRPYVDYLFPGVGPHGPGHQHQHPPKGYGASVEAAGAHFPLQGAHYGRPHLLVPGEPLGYGVQRSPSFQSKTPPETGGYASLPTKGQGGPPGAGLAFPPPAAGLYVPHPHHKQAGPAAHQLHVLGSRSQVFASDSPPQSLLTPSRNSLNVDLYELGSTSVQQWPAATLARRDSLQKPGLEAPPRAHVAFRPDCPVPSRTNSFNSHQPRPGPPGKAEPSLPAPNTVTAVTAAHILHPVKSVRVLRPEPQTAVGPSHPAWVPAPAPAPAPAPAPAAEGLDAKEEHALALGGAGAFPLDVEYGGPDRRCPPPPYPKHLLLRSKSEQYDLDSLCAGMEQSLRAGPNEPEGGDKSRKSAKGDKGGKDKKQIQTSPVPVRKNSRDEEKRESRIKSYSPYAFKFFMEQHVENVIKTYQQKVNRRLQLEQEMAKAGLCEAEQEQMRKILYQKESNYNRLKRAKMDKSMFVKIKTLGIGAFGEVCLACKVDTHALYAMKTLRKKDVLNRNQVAHVKAERDILAEADNEWVVKLYYSFQDKDSLYFVMDYIPGGDMMSLLIRMEVFPEHLARFYIAELTLAIESVHKMGFIHRDIKPDNILIDLDGHIKLTDFGLCTGFRWTHNSKYYQKGSHVRQDSMEPSDLWDDVSNCRCGDRLKTLEQRARKQHQRCLAHSLVGTPNYIAPEVLLRKGYTQLCDWWSVGVILFEMLVGQPPFLAPTPTETQLKVINWENTLHIPAQVKLSPEARDLITKLCCSADHRLGRNGADDLKAHPFFSAIDFSSDIRKQPAPYVPTISHPMDTSNFDPVDEESPWNDASEGSTKAWDTLTSPNNKHPEHAFYEFTFRRFFDDNGYPFRCPKPSGAEASQAESSDLESSDLVDQTEGCQPVYV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Horse
91
Rabbit
91
Bovine
82
Sheep
82
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95835/Q9NRM7 As Substrate

Site PTM Type Enzyme
T17 Phosphorylation
Y23 Phosphorylation
S26 Phosphorylation
S27 Phosphorylation
S37 Phosphorylation
S42 Phosphorylation
K76 Ubiquitination
S84 Phosphorylation
Y143 Phosphorylation
S171 Phosphorylation
S177 Phosphorylation
S181 Phosphorylation
R243 Methylation
S244 Phosphorylation
T246 Phosphorylation
R252 Methylation
T255 Phosphorylation
R259 Methylation
T261 Phosphorylation
T262 Phosphorylation
K275 Methylation
Y277 Phosphorylation
S278 Phosphorylation
Y283 Phosphorylation
S336 Phosphorylation
S462 Phosphorylation
S464 Phosphorylation O60285 (NUAK1) , Q9H093 (NUAK2)
T490 Phosphorylation P06493 (CDK1)
S580 Phosphorylation
S596 Phosphorylation
T612 Phosphorylation
S613 Phosphorylation P06493 (CDK1)
T616 Phosphorylation
S633 Phosphorylation
S635 Phosphorylation
K662 Ubiquitination
S674 Phosphorylation O95835 (LATS1)
K688 Ubiquitination
K728 Ubiquitination
K734 Ubiquitination
K751 Sumoylation
S792 Phosphorylation
K830 Sumoylation
S864 Phosphorylation
S872 Phosphorylation Q13188 (STK3)
S909 Phosphorylation Q13043 (STK4) , Q13188 (STK3) , O95835 (LATS1)
T967 Phosphorylation Q13188 (STK3)
K976 Ubiquitination
S982 Phosphorylation
K1005 Ubiquitination
T1012 Phosphorylation Q13188 (STK3)
K1029 Ubiquitination
K1046 Ubiquitination
S1049 Phosphorylation O95835 (LATS1)
T1060 Phosphorylation Q13188 (STK3)
Y1076 Phosphorylation
T1079 Phosphorylation Q13043 (STK4) , Q13188 (STK3)
S1107 Phosphorylation
S1111 Phosphorylation
Site PTM Type Enzyme
T5 Phosphorylation
T9 Phosphorylation
T10 Phosphorylation
Y11 Phosphorylation
S12 Phosphorylation
S43 Phosphorylation
K50 Ubiquitination
K55 Acetylation
K55 Ubiquitination
Y82 Phosphorylation
S83 Phosphorylation O14965 (AURKA)
Y135 Phosphorylation
K154 Ubiquitination
S172 Phosphorylation O14757 (CHEK1) , O96017 (CHEK2)
S274 Phosphorylation
K278 Ubiquitination
T279 Phosphorylation
Y286 Phosphorylation
S288 Phosphorylation
S342 Phosphorylation
S380 Phosphorylation O14965 (AURKA) , O14757 (CHEK1) , O96017 (CHEK2)
S408 Phosphorylation O96017 (CHEK2) , O14757 (CHEK1)
S446 Phosphorylation O96017 (CHEK2) , O14757 (CHEK1)
S528 Phosphorylation
Y531 Phosphorylation
S535 Phosphorylation
S543 Phosphorylation
K565 Acetylation
K570 Acetylation
K571 Acetylation
T575 Phosphorylation
S576 Phosphorylation
S598 Phosphorylation
K620 Ubiquitination
K651 Ubiquitination
T690 Phosphorylation
K697 Ubiquitination
K793 Ubiquitination
K827 Ubiquitination
S829 Phosphorylation
S835 Phosphorylation Q9NRM7 (LATS2) , O14757 (CHEK1)
S872 Phosphorylation Q13043 (STK4) , Q13188 (STK3)
K939 Ubiquitination
K950 Ubiquitination
K968 Ubiquitination
K984 Ubiquitination
T1024 Phosphorylation
T1026 Phosphorylation
S1027 Phosphorylation
Y1038 Phosphorylation
T1041 Phosphorylation Q13188 (STK3) , Q13043 (STK4)
Y1051 Phosphorylation

PTMs - O95835/Q9NRM7 As Enzyme

Substrate Site Source
O14974 (PPP1R12A) S445 Uniprot
O95835 (LATS1) S674 Uniprot
O95835 (LATS1) S909 Uniprot
O95835 (LATS1) S1049 Uniprot
P04049 (RAF1) S259 Uniprot
P46937-2 (YAP1) S61 Uniprot
P46937-2 (YAP1) S109 Uniprot
P46937 (YAP1) S127 Uniprot
P46937-2 (YAP1) S164 Uniprot
P46937-2 (YAP1) S381 Uniprot
P46937 (YAP1) S397 Uniprot
Q02241 (KIF23) S716 Uniprot
Q02241 (KIF23) S814 Uniprot
Q8NHZ8 (CDC26) T7 Uniprot
Q9GZV5 (WWTR1) S89 Uniprot
Q9NQS7 (INCENP) S894 Uniprot
Substrate Site Source
O95863 (SNAI1) T203 Uniprot
P00519-2 (ABL1) T197 Uniprot
P38936 (CDKN1A) S146 Uniprot
P46937 (YAP1) S127 Uniprot
P46937 (YAP1) S397 Uniprot
P61981 (YWHAG) S59 Uniprot
Q02241 (KIF23) S716 Uniprot
Q02241 (KIF23) S814 Uniprot
Q4VCS5 (AMOT) S175 Uniprot
Q8NHZ8 (CDC26) T7 Uniprot
Q9GZV5 (WWTR1) S89 Uniprot
Q9NQS7 (INCENP) S894 Uniprot
Q9NRM7 (LATS2) S835 Uniprot
Q9Y2J4 (AMOTL2) S159 Uniprot

Research Backgrounds

Function:

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. Plays a role in mammary gland epithelial cells differentiation, both through the Hippo signaling pathway and the intracellular estrogen receptor signaling pathway by promoting the degradation of ESR1.

PTMs:

Autophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all adult tissues examined except for lung and kidney.

Subunit Structure:

Complexes with CDK1 in early mitosis. LATS1-associated CDK1 has no mitotic cyclin partner and no apparent kinase activity. Binds phosphorylated ZYX, locating this protein to the mitotic spindle and suggesting a role for actin regulatory proteins during mitosis. Binds to and colocalizes with LIMK1 at the actomyosin contractile ring during cytokinesis. Interacts (via PPxY motif 2) with YAP1 (via WW domains). Interacts with MOB1A and MOB1B. Interacts with LIMD1, WTIP and AJUBA. Interacts with ESR1, DCAF1 and DCAF13; probably recruits DCAF1 and DCAF13 to ESR1 to promote ESR1 ubiquitination and ubiquitin-mediated proteasomal degradation. Interacts with STK3/MST2; this interaction is inhibited in the presence of DLG5. Interacts with SCRIB in the presence of DLG5.

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Function:

Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. Phosphorylates SNAI1 in the nucleus leading to its nuclear retention and stabilization, which enhances its epithelial-mesenchymal transition and tumor cell invasion/migration activities. This tumor-promoting activity is independent of its effects upon YAP1 or WWTR1/TAZ.

PTMs:

Autophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3/MST2.

Subcellular Location:

Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm. Cytoplasm>Cytoskeleton>Spindle pole. Nucleus.
Note: Colocalizes with AURKA at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis. Translocates to the nucleus upon mitotic stress by nocodazole treatment.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined.

Subunit Structure:

Interacts with and is phosphorylated by AURKA. Binds to AR. Interacts with AJUBA during mitosis and this complex regulates organization of the spindle apparatus through recruitment of gamma-tubulin to the centrosome. Interacts (via PPxY motif) with YAP1 (via WW domains). Interacts with MOB1A and MOB1B. Interacts with LIMD1, WTIP and AJUBA. Interacts with SNAI1.

Family&Domains:

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

Research Fields

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species.   (View pathway)

References

1). Unveiling the mechanism of photothermal therapy in acne man-agement: targeting sebaceous gland ferroptosis via umbilical cord mesenchymal stem cell membrane-encapsulated Au-Ag-PDA. Frontiers in bioengineering and biotechnology, 2024 (PubMed: 38915336) [IF=5.7]
2). Anti-cancer effects of nitazoxanide in epithelial ovarian cancer in-vitro and in-vivo. Chemico-biological interactions, 2024 (PubMed: 39084502) [IF=5.1]

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