CCDC99 Antibody - #AF0628
| Product: | CCDC99 Antibody |
| Catalog: | AF0628 |
| Description: | Rabbit polyclonal antibody to CCDC99 |
| Application: | WB IF/ICC |
| Reactivity: | Human, Mouse |
| Prediction: | Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 70kDa; 70kD(Calculated). |
| Uniprot: | Q96EA4 |
| RRID: | AB_2834382 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF0628, RRID:AB_2834382.
Fold/Unfold
AA409762; Arsenite related gene 1 protein; Arsenite-related gene 1 protein; CCDC 99; Ccdc99; Coiled coil domain containing 99; Coiled-coil domain-containing protein 99; FLJ20364; FLJ40690; hSpindly; MGC124977; Protein Spindly; rhabdomyosarcoma antigen MU RMS 40.4A; Rhabdomyosarcoma antigen MU-RMS-40.4A; Rrhabdomyosarcoma antigen protein MU RMS 40.4A; SPDL1; SPDLY_HUMAN; Spindle apparatus coiled coil domain containing protein 1; Spindle apparatus coiled coil protein 1; Spindly; Spindly homolog;
Immunogens
- Q96EA4 SPDLY_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEADIITNLRCRLKEAEEERLKAAQYGLQLVESQNELQNQLDKCRNEMMTMTESYEQEKYTLQREVELKSRMLESLSCECEAIKQQQKMHLEKLEEQLSRSHGQEVNELKTKIEKLKVELDEARLSEKQLKHQVDHQKELLSCKSEELRVMSERVQESMSSEMLALQIELTEMESMKTTLKEEVNELQYRQEQLELLITNLMRQVDRLKEEKEEREKEAVSYYNALEKARVANQDLQVQLDQALQQALDPNSKGNSLFAEVEDRRAAMERQLISMKVKYQSLKKQNVFNREQMQRMKLQIATLLQMKGSQTEFEQQERLLAMLEQKNGEIKHLLGEIRNLEKFKNLYDSMESKPSVDSGTLEDNTYYTDLLQMKLDNLNKEIESTKGELSIQRMKALFESQRALDIERKLFANERCLQLSESENMKLRAKLDELKLKYEPEETVEVPVLKKRREVLPVDITTAKDACVNNSALGGEVYRLPPQKEETQSCPNSLEDNNLQLEKSVSIYTPVVSLSPHKNLPVDMQLKKEKKCVKLIGVPADAEALSERSGNTPNSPRLAAESKLQTEVKEGKETSSKLEKETCKKLHPILYVSSKSTPETQCPQQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96EA4 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| S145 | Phosphorylation | Uniprot | |
| R154 | Methylation | Uniprot | |
| K228 | Ubiquitination | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| Y347 | Phosphorylation | Uniprot | |
| K374 | Ubiquitination | Uniprot | |
| K409 | Ubiquitination | Uniprot | |
| T461 | Phosphorylation | Uniprot | |
| S471 | Phosphorylation | Uniprot | |
| T487 | Phosphorylation | Uniprot | |
| S493 | Phosphorylation | Uniprot | |
| T509 | Phosphorylation | Uniprot | |
| S513 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S515 | Phosphorylation | P24941 (CDK2) | Uniprot |
| S546 | Phosphorylation | Uniprot | |
| S555 | Phosphorylation | Uniprot | |
| S562 | Phosphorylation | Uniprot | |
| T566 | Phosphorylation | Uniprot | |
| S594 | Phosphorylation | Uniprot | |
| T600 | Phosphorylation | Uniprot |
Research Backgrounds
Required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track).
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Chromosome>Centromere>Kinetochore. Nucleus. Cytoplasm>Cytoskeleton>Spindle pole.
Note: Localizes to the nucleus in interphase and to the kinetochore in early prometaphase. Relocalizes to the mitotic spindle pole before metaphase and is subsequently lost from the spindle poles after chromosome congression is completed. Removal of this protein from the kinetochore requires the dynein/dynactin complex.
Interacts with KNTC1 and ZW10. These interactions appear weak and may be transient or indirect. Interacts with dynein intermediate chain and dynactin (DCTN1).
Belongs to the Spindly family.
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