TXNIP Antibody - #DF7506

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Product: TXNIP Antibody
Catalog: DF7506
Description: Rabbit polyclonal antibody to TXNIP
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 50 kDa; 44kD(Calculated).
Uniprot: Q9H3M7
RRID: AB_2841006

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
TXNIP Antibody detects endogenous levels of total TXNIP.
RRID:
AB_2841006
Cite Format: Affinity Biosciences Cat# DF7506, RRID:AB_2841006.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EST01027; HHCPA78; THIF; Thioredoxin binding protein 2; Thioredoxin interacting protein; Thioredoxin-binding protein 2; Thioredoxin-interacting protein; TXNIP; TXNIP_HUMAN; Upregulated by 1,25 dihydroxyvitamin D 3; VDUP1; Vitamin D3 up-regulated protein 1;

Immunogens

Immunogen:
Uniprot:

Q9H3M7(TXNIP_HUMAN) >>Visit HPA database.

Gene(ID):
TXNIP(10628)
Sequence:
MVMFKKIKSFEVVFNDPEKVYGSGEKVAGRVIVEVCEVTRVKAVRILACGVAKVLWMQGSQQCKQTSEYLRYEDTLLLEDQPTGENEMVIMRPGNKYEYKFGFELPQGPLGTSFKGKYGCVDYWVKAFLDRPSQPTQETKKNFEVVDLVDVNTPDLMAPVSAKKEKKVSCMFIPDGRVSVSARIDRKGFCEGDEISIHADFENTCSRIVVPKAAIVARHTYLANGQTKVLTQKLSSVRGNHIISGTCASWRGKSLRVQKIRPSILGCNILRVEYSLLIYVSVPGSKKVILDLPLVIGSRSGLSSRTSSMASRTSSEMSWVDLNIPDTPEAPPCYMDVIPEDHRLESPTTPLLDDMDGSQDSPIFMYAPEFKFMPPPTYTEVDPCILNNNVQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
82
Horse
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H3M7 As Substrate

Site PTM Type Enzyme
K8 Sumoylation
S9 Phosphorylation
K19 Sumoylation
K19 Ubiquitination
K26 Ubiquitination
K53 Ubiquitination
S60 Phosphorylation
K64 Ubiquitination
K96 Ubiquitination
K100 Sumoylation
K100 Ubiquitination
K115 Sumoylation
K115 Ubiquitination
K117 Sumoylation
K117 Ubiquitination
K140 Sumoylation
K140 Ubiquitination
K141 Ubiquitination
K163 Sumoylation
K163 Ubiquitination
K164 Ubiquitination
K167 Sumoylation
K187 Ubiquitination
K212 Sumoylation
K212 Ubiquitination
K228 Sumoylation
K228 Ubiquitination
K233 Sumoylation
K233 Ubiquitination
K259 Ubiquitination
K287 Sumoylation
K287 Ubiquitination
S303 Phosphorylation
S304 Phosphorylation
S307 Phosphorylation
S308 Phosphorylation
S318 Phosphorylation
T348 Phosphorylation
T349 Phosphorylation
S358 Phosphorylation
S361 Phosphorylation
T377 Phosphorylation
Y378 Phosphorylation
T379 Phosphorylation

Research Backgrounds

Function:

May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1).

PTMs:

Ubiquitinated; undergoes polyubiquitination catalyzed by ITCH resulting in proteasomal degradation.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer; disulfide-linked. Interacts with TXN/thioredoxin through its redox-active site. Interacts with transcriptional repressors ZBTB16, ZBTB32 and HDAC1. Interacts (via C-terminus) with ITCH (via WW domains). Interacts with DDIT4.

Family&Domains:

Belongs to the arrestin family.

Research Fields

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

References

1). Geranylgeranylacetone Ameliorates Skin Inflammation by Regulating and Inducing Thioredoxin via the Thioredoxin Redox System. Antioxidants, 2023 (PubMed: 37760004) [IF=7.0]

Application: IHC    Species: Mouse    Sample:

Figure 1 Induction of TRX and Nrf2 by GGA in the ICD model. (A,B) Immunohistochemistry was performed to detect the effects of GGA on TRX induction and Nrf2 activation. GGA induces the production of TRX, especially after oral administration. Normal = mice without any treatment. NS = normal saline; bars 100 μm. The most pronounced nuclear translocation of Nrf2 was in the GGA gavage-treated group, followed by the GGA topically applied group; bars 50 μm; n = 5 mice for each group. Cells with nuclear translocation are indicated by arrows. (C,D) The mean of IOD expression of TRX and Nrf2. Values are shown as the mean ± SD (* p < 0.05, ** p < 0.01, **** p < 0.0001; one-way ANOVA with post hoc Tukey’s).
2). Sonneratia apetala seed oil attenuates potassium oxonate/hypoxanthine-induced hyperuricemia and renal injury in mice. Food & Function, 2021 (PubMed: 34606558) [IF=6.1]
3). Chrysophanol exerts a protective effect against Aβ25-35-induced Alzheimer’s disease model through regulating the ROS/TXNIP/NLRP3 pathway. Inflammopharmacology, 2023 (PubMed: 36976486) [IF=5.8]
4). Polygalasaponin F ameliorates middle cerebral artery occlusion-induced focal ischemia / reperfusion injury in rats through inhibiting TXNIP/NLRP3 signaling pathway. Journal of neuroimmunology, 2024 (PubMed: 38198981) [IF=3.3]

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