TXNIP Antibody | Affinity Biosciences

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Western blot analysis of extracts from rat brain, using TXNIP Antibody.

Figure 1 Induction of TRX and Nrf2 by GGA in the ICD model.

Product:	TXNIP Antibody
Catalog:	DF7506
Description:	Rabbit polyclonal antibody to TXNIP
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	50 kDa; 44kD(Calculated).
Uniprot:	Q9H3M7
RRID:	AB_2841006

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(82%)

Clonality:

Polyclonal

Specificity:

TXNIP Antibody detects endogenous levels of total TXNIP.

RRID:

AB_2841006
Cite Format: Affinity Biosciences Cat# DF7506, RRID:AB_2841006.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

EST01027; HHCPA78; THIF; Thioredoxin binding protein 2; Thioredoxin interacting protein; Thioredoxin-binding protein 2; Thioredoxin-interacting protein; TXNIP; TXNIP_HUMAN; Upregulated by 1,25 dihydroxyvitamin D 3; VDUP1; Vitamin D3 up-regulated protein 1;

Immunogens

Immunogen:

Uniprot:

Q9H3M7(TXNIP_HUMAN) >>Visit HPA database.

Gene(ID):

TXNIP(10628)

Sequence:

Q9H3M7 TXNIP_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MVMFKKIKSFEVVFNDPEKVYGSGEKVAGRVIVEVCEVTRVKAVRILACGVAKVLWMQGSQQCKQTSEYLRYEDTLLLEDQPTGENEMVIMRPGNKYEYKFGFELPQGPLGTSFKGKYGCVDYWVKAFLDRPSQPTQETKKNFEVVDLVDVNTPDLMAPVSAKKEKKVSCMFIPDGRVSVSARIDRKGFCEGDEISIHADFENTCSRIVVPKAAIVARHTYLANGQTKVLTQKLSSVRGNHIISGTCASWRGKSLRVQKIRPSILGCNILRVEYSLLIYVSVPGSKKVILDLPLVIGSRSGLSSRTSSMASRTSSEMSWVDLNIPDTPEAPPCYMDVIPEDHRLESPTTPLLDDMDGSQDSPIFMYAPEFKFMPPPTYTEVDPCILNNNVQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Horse

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H3M7 As Substrate

Q9H3M7

Site	PTM Type	Source
K8	Sumoylation	Uniprot
S9	Phosphorylation	Uniprot
K19	Sumoylation	Uniprot
K19	Ubiquitination	Uniprot
K26	Ubiquitination	Uniprot
K53	Ubiquitination	Uniprot
S60	Phosphorylation	Uniprot
K64	Ubiquitination	Uniprot
K96	Ubiquitination	Uniprot
K100	Sumoylation	Uniprot
K100	Ubiquitination	Uniprot
K115	Sumoylation	Uniprot
K115	Ubiquitination	Uniprot
K117	Sumoylation	Uniprot
K117	Ubiquitination	Uniprot
K140	Sumoylation	Uniprot
K140	Ubiquitination	Uniprot
K141	Ubiquitination	Uniprot
K163	Sumoylation	Uniprot
K163	Ubiquitination	Uniprot
K164	Ubiquitination	Uniprot
K167	Sumoylation	Uniprot
K187	Ubiquitination	Uniprot
K212	Sumoylation	Uniprot
K212	Ubiquitination	Uniprot
K228	Sumoylation	Uniprot
K228	Ubiquitination	Uniprot
K233	Sumoylation	Uniprot
K233	Ubiquitination	Uniprot
K259	Ubiquitination	Uniprot
K287	Sumoylation	Uniprot
K287	Ubiquitination	Uniprot
S303	Phosphorylation	Uniprot
S304	Phosphorylation	Uniprot
S307	Phosphorylation	Uniprot
S308	Phosphorylation	Uniprot
S318	Phosphorylation	Uniprot
T348	Phosphorylation	Uniprot
T349	Phosphorylation	Uniprot
S358	Phosphorylation	Uniprot
S361	Phosphorylation	Uniprot
T377	Phosphorylation	Uniprot
Y378	Phosphorylation	Uniprot
T379	Phosphorylation	Uniprot

Research Backgrounds

Q9H3M7_TXNIP_HUMAN

Function:

May act as an oxidative stress mediator by inhibiting thioredoxin activity or by limiting its bioavailability. Interacts with COPS5 and restores COPS5-induced suppression of CDKN1B stability, blocking the COPS5-mediated translocation of CDKN1B from the nucleus to the cytoplasm. Functions as a transcriptional repressor, possibly by acting as a bridge molecule between transcription factors and corepressor complexes, and over-expression will induce G0/G1 cell cycle arrest. Required for the maturation of natural killer cells. Acts as a suppressor of tumor cell growth. Inhibits the proteasomal degradation of DDIT4, and thereby contributes to the inhibition of the mammalian target of rapamycin complex 1 (mTORC1).

PTMs:

Ubiquitinated; undergoes polyubiquitination catalyzed by ITCH resulting in proteasomal degradation.

Subcellular Location:

Cytoplasm.

Subunit Structure:

Homodimer; disulfide-linked. Interacts with TXN/thioredoxin through its redox-active site. Interacts with transcriptional repressors ZBTB16, ZBTB32 and HDAC1. Interacts (via C-terminus) with ITCH (via WW domains). Interacts with DDIT4.

Family&Domains:

Belongs to the arrestin family.

Research Fields

· Organismal Systems > Immune system > NOD-like receptor signaling pathway. (View pathway)

References

1). Geranylgeranylacetone Ameliorates Skin Inflammation by Regulating and Inducing Thioredoxin via the Thioredoxin Redox System. Antioxidants, 2023 (PubMed: 37760004) [IF=7.0]

Application: IHC Species: Mouse Sample:

Figure 1 Induction of TRX and Nrf2 by GGA in the ICD model. (A,B) Immunohistochemistry was performed to detect the effects of GGA on TRX induction and Nrf2 activation. GGA induces the production of TRX, especially after oral administration. Normal = mice without any treatment. NS = normal saline; bars 100 μm. The most pronounced nuclear translocation of Nrf2 was in the GGA gavage-treated group, followed by the GGA topically applied group; bars 50 μm; n = 5 mice for each group. Cells with nuclear translocation are indicated by arrows. (C,D) The mean of IOD expression of TRX and Nrf2. Values are shown as the mean ± SD (* p < 0.05, ** p < 0.01, **** p < 0.0001; one-way ANOVA with post hoc Tukey’s).

2). Sonneratia apetala seed oil attenuates potassium oxonate/hypoxanthine-induced hyperuricemia and renal injury in mice. Food & Function, 2021 (PubMed: 34606558) [IF=6.1]

3). Chrysophanol exerts a protective effect against Aβ25-35-induced Alzheimer’s disease model through regulating the ROS/TXNIP/NLRP3 pathway. Inflammopharmacology, 2023 (PubMed: 36976486) [IF=5.8]

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TXNIP Antibody - #DF7506