Occludin Antibody | Affinity Biosciences

Product:	Occludin Antibody
Catalog:	DF7504
Source:	Rabbit
Application:	WB, IHC, IF/ICC
Reactivity:	Human, Mouse, Rat, Pig
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	23~30kD, 52~65kD; 59kD(Calculated).
Uniprot:	Q16625
RRID:	AB_2841004

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Cat.#S0018

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Pig

Prediction:

Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(80%)

Clonality:

Polyclonal

Specificity:

Occludin Antibody detects endogenous levels of total Occludin.

RRID:

AB_2841004
Cite Format: Affinity Biosciences Cat# DF7504, RRID:AB_2841004.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

BLCPMG; FLJ08163; FLJ18079; FLJ77961; FLJ94056; MGC34277; Occludin; Ocln; OCLN_HUMAN; Tight junction protein occludin;

Immunogens

Immunogen:

Uniprot:

Q16625(OCLN_HUMAN) >>Visit HPA database.

Gene(ID):

OCLN(100506658)

Expression:

Q16625 OCLN_HUMAN:

Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.

Sequence:

Q16625 OCLN_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSSRPLESPPPYRPDEFKPNHYAPSNDIYGGEMHVRPMLSQPAYSFYPEDEILHFYKWTSPPGVIRILSMLIIVMCIAIFACVASTLAWDRGYGTSLLGGSVGYPYGGSGFGSYGSGYGYGYGYGYGYGGYTDPRAAKGFMLAMAAFCFIAALVIFVTSVIRSEMSRTRRYYLSVIIVSAILGIMVFIATIVYIMGVNPTAQSSGSLYGSQIYALCNQFYTPAATGLYVDQYLYHYCVVDPQEAIAIVLGFMIIVAFALIIFFAVKTRRKMDRYDKSNILWDKEHIYDEQPPNVEEWVKNVSAGTQDVPSPPSDYVERVDSPMAYSSNGKVNDKRFYPESSYKSTPVPEVVQELPLTSPVDDFRQPRYSSGGNFETPSKRAPAKGRAGRSKRTEQDHYETDYTTGGESCDELEEDWIREYPPITSDQQRQLYKRNFDTGLQEYKSLQSELDEINKELSRLDKELDDYREESEEYMAAADEYNRLKQVKGSADYKSKKNHCKQLKSKLSHIKKMVGDYDRQKT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16625 As Substrate

Q16625

Site	PTM Type	Enzyme	Source
S8	Phosphorylation		Uniprot
K18	Ubiquitination		Uniprot
S40	Phosphorylation		Uniprot
Y44	Phosphorylation		Uniprot
S45	Phosphorylation		Uniprot
K276	Ubiquitination		Uniprot
S277	Phosphorylation		Uniprot
K283	Ubiquitination		Uniprot
Y287	Phosphorylation		Uniprot
K299	Ubiquitination		Uniprot
S302	Phosphorylation		Uniprot
T305	Phosphorylation		Uniprot
S310	Phosphorylation		Uniprot
S313	Phosphorylation		Uniprot
Y315	Phosphorylation		Uniprot
S321	Phosphorylation		Uniprot
Y325	Phosphorylation		Uniprot
S326	Phosphorylation		Uniprot
S327	Phosphorylation		Uniprot
K330	Ubiquitination		Uniprot
Y337	Phosphorylation		Uniprot
S340	Phosphorylation		Uniprot
S341	Phosphorylation		Uniprot
Y342	Phosphorylation		Uniprot
K343	Ubiquitination		Uniprot
S344	Phosphorylation		Uniprot
T345	Phosphorylation		Uniprot
S358	Phosphorylation		Uniprot
Y368	Phosphorylation		Uniprot
S369	Phosphorylation		Uniprot
S370	Phosphorylation		Uniprot
T376	Phosphorylation		Uniprot
S378	Phosphorylation		Uniprot
K379	Ubiquitination		Uniprot
T393	Phosphorylation		Uniprot
Y398	Phosphorylation	P12931 (SRC)	Uniprot
T400	Phosphorylation	P68400 (CSNK2A1)	Uniprot
Y402	Phosphorylation	P12931 (SRC)	Uniprot
T403	Phosphorylation	Q02156 (PRKCE) , P24723 (PRKCH) , Q05513 (PRKCZ)	Uniprot
T404	Phosphorylation	Q02156 (PRKCE) , Q05513 (PRKCZ) , P24723 (PRKCH) , P68400 (CSNK2A1) , P67870 (CSNK2B)	Uniprot
S408	Phosphorylation	P67870 (CSNK2B) , P68400 (CSNK2A1)	Uniprot
T424	Phosphorylation	Q02156 (PRKCE) , Q05513 (PRKCZ)	Uniprot
T438	Phosphorylation	Q02156 (PRKCE) , Q05513 (PRKCZ)	Uniprot
Y443	Phosphorylation		Uniprot
S445	Phosphorylation		Uniprot
S448	Phosphorylation		Uniprot
S458	Phosphorylation		Uniprot
Y467	Phosphorylation		Uniprot
S471	Phosphorylation		Uniprot
Y474	Phosphorylation		Uniprot
Y481	Phosphorylation		Uniprot
K488	Ubiquitination		Uniprot
S490	Phosphorylation	P05771 (PRKCB)	Uniprot

Research Backgrounds

Q16625_OCLN_HUMAN

Function:

May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.

PTMs:

Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell junction>Tight junction.

Tissue Specificity:

Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in kidney. Not detected in testis.

Subunit Structure:

Interacts with TJP1/ZO1. Interacts with VAPA. Interacts with CLDN1, CLDN6, CLDN9, CLDN11, CLDN12 and CLDN17.

Family&Domains:

The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By similarity). The first extracellular loop participates in an adhesive interaction.

Belongs to the ELL/occludin family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pathogenic Escherichia coli infection.

· Human Diseases > Infectious diseases: Viral > Hepatitis C.

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

References

1). Li X et al. Multistage-Responsive Nanocomplexes Attenuate Ulcerative Colitis by Improving the Accumulation and Distribution of Oral Nucleic Acid Drugs in the Colon. ACS Appl Mater Interfaces 2022 Jan 12;14(1):2058-2070. (PubMed: 34978415) [IF=10.383]

2). Wang Y et al. Fourteen composite probiotics alleviate type 2 diabetes through modulating gut microbiota and modifying M1/M2 phenotype macrophage in db/ db mice. Pharmacol Res 2020 Nov;161:105150. (PubMed: 32818655) [IF=10.334]

Application: IHC Species: mouse Sample: colon

Fig. 4.| Effects of the composite probiotics on intestinal barrier function.The protein expression of claudin-1 (G), occludin-1 (H), ZO-1 (I) and mucin-2 (J)determined by immunohistochemistry (original magnification 200×) (n = 4 images/group).

3). Li C et al. Oxyberberine, a novel gut microbiota-mediated metabolite of berberine, possesses superior anti-colitis effect: Impact on intestinal epithelial barrier, gut microbiota profile and TLR4-MyD88-NF-κB pathway. Pharmacol Res 2020 Feb;152:104603 (PubMed: 31863867) [IF=10.334]

Application: WB Species: Mice Sample: colonic tissues

Fig. 4. OBB protected intestinal epithelial barrier by modulating TJs proteins. Effect of OBB on the mRNA levels of mucin-1(A) and mucin-2 (B). (C) Representative Western blotting images of TJs protein, and the relative protein expressions were normalized to β-actin. (D-H) Changes in the relative protein expression levels of ZO-1, ZO-2, occludin, JAM-A, and claudin-1 were measured respectively. Data are shown as mean ± SEM (n = 3). # P < 0.05, ## P < 0.01 vs. Control group, * P < 0.05, ** P < 0.01 vs. DSS group, & P < 0.05, && P < 0.01 vs. BBR group.

4). Liu L et al. Extracellular vesicles of Fusobacterium nucleatum compromise intestinal barrier through targeting RIPK1-mediated cell death pathway. Gut Microbes 2021;13(1):1-20. (PubMed: 33769187) [IF=9.434]

Application: IHC Species: mouse Sample: colon

Figure 5. |FnEVs increase gut barrier leakage in experimental colitis models. (a) Representative images and ex vivo imaging with the intestine, liver, heart, spleen and kidney of mice. (b) Relative fluorescence intensity of translocated EGFP-labeled E.coli in every tissues. (c) Representative images of immunohistochemical stainings of ZO-1, claudin-1 and occludin in the colon on day 3 after colitis induction. Scale bar = 50 um.

5). Xiao H et al. Tremella fuciformis polysaccharides ameliorated ulcerative colitis via inhibiting inflammation and enhancing intestinal epithelial barrier function. Int J Biol Macromol 2021 Mar 17;180:633-642. (PubMed: 33744251) [IF=8.025]

Application: IF/ICC Species: mouse Sample: colon

Fig. 3. |Effect of TFP on intestinal barrier in DSS-induced colitis mice. (A) Representative TEM images in colon tissues (white arrows indicate TJs, black arrows indicate intestinal villi loss).(B) The mRNA level of TJP1 in colon tissues. (C) The mRNA level of OCLN in colon tissues. (D) The fluorescence intensity of ZO-1. (E) The fluorescence intensity of occludin.(F) Representative fluorescent images of ZO-1 and occludin in colon tissues. All data were shown as mean ± SEM. (n = 6 for each group). ⁎P < 0.05. ⁎⁎P < 0.01 compared with control group; #P < 0.05. ##P < 0.01 compared with DSS group.

6). Zhao C et al. A fiber-enriched diet alleviates Staphylococcus aureus-induced mastitis by activating the HDAC3-mediated antimicrobial program in macrophages via butyrate production in mice. PLoS Pathog 2023 Jan 19;19(1):e1011108. (PubMed: 36656870) [IF=7.464]

7). Deng Z et al. Gly-Pro-Ala peptide and FGSHF3 exert protective effects in DON-induced toxicity and intestinal damage via decreasing oxidative stress. Food Res Int 2021 Jan;139:109840. (PubMed: 33509464) [IF=7.425]

8). Wu J et al. Patchouli alcohol attenuates 5-fluorouracil-induced intestinal mucositis via TLR2/MyD88/NF-kB pathway and regulation of microbiota. Biomed Pharmacother 2020 Jan 28;124:109883 (PubMed: 32004938) [IF=7.419]

Application: WB Species: rat Sample: Intestinal

Fig. 4. |Effect of PA on intestinal mocusal barrier proteins. (a–d) Relative mRNA expression of MLCK, ZO-1, occludin and claudin-1 (n = 4); (e–i) Expressions of MLC,p-MLC, ZO-1, occludin and claudin-1proteins (n = 3). Values were represented the mean ± SEM. **P < 0.01, *P < 0.05 versus 5-FU group and ##P < 0.01 versus normal group.

9). Zhan X et al. Polysaccharides from Garlic Protect against Liver Injury in DSS-Induced Inflammatory Bowel Disease of Mice via Suppressing Pyroptosis and Oxidative Damage. Oxid Med Cell Longev 2022 Aug 16;2022:2042163. (PubMed: 36017235) [IF=7.310]

Application: WB Species: Mice Sample: colon tissues

Figure 4 PSG improved the intestinal barrier function of IBD mice. (a) Effect of PSG on MPO level in the colon of IBD mice. (b) Effect of PSG on DAO level in the colon of IBD mice. The expressions of tight junction protein ZO1 and occludin in the colon tissues of IBD mice were evaluated by Western blot assay (c) and quantitative analysis for Western blot results (n = 3) (d). (e) The expression of MUC2 in the colon tissues of IBD mice was detected by IHC. Results were expressed as the mean ± SEM. ∗p < 0.05 and ∗∗p < 0.01 vs. the control group; #p < 0.05 and ##p < 0.01 vs. the model group (DSS only).

10). Tang W et al. The lncRNA-AK046375 Upregulates Metallothionein-2 by Sequestering miR-491-5p to Relieve the Brain Oxidative Stress Burden after Traumatic Brain Injury. Oxid Med Cell Longev 2022 Feb 16;2022:8188404. (PubMed: 35222805) [IF=7.310]

Application: WB Species: Mice Sample:

Figure 7 AK046375 maintains BBB integrity and decreases brain water content in mice on 7 days after TBI. (a) Western blotting results of ZO1, occludin, and claudin-5 in each group and quantification (n = 6/group, mean ± SD). (b) Brain water content in each group after TBI (n = 6/group, mean ± SD). (c, d) Fluorescence of Evans blue and Evans blue extravasation in each group around the injury site (n = 12/group, mean ± SD) (scale bar = 50 μm, 200x) (∗P < 0.05 vs. the overexpression vector group, &P < 0.05 vs. the knockdown vector group by one-way ANOVA).

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Occludin Antibody - #DF7504