Nephrin Antibody - #DF7501

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Product: Nephrin Antibody
Catalog: DF7501
Description: Rabbit polyclonal antibody to Nephrin
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 135kD,180kD; 135kD(Calculated).
Uniprot: O60500
RRID: AB_2841001

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(90%), Zebrafish(100%), Bovine(80%), Horse(100%), Sheep(80%), Rabbit(80%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Nephrin Antibody detects endogenous levels of total Nephrin.
RRID:
AB_2841001
Cite Format: Affinity Biosciences Cat# DF7501, RRID:AB_2841001.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CNF; Nephrin; Nephrosis 1 congenital Finnish type; Nephrosis 1, congenital, Finnish type (nephrin); NPHN; NPHN_HUMAN; NPHS 1; Nphs1; Renal glomerulus specific cell adhesion receptor; Renal glomerulus-specific cell adhesion receptor;

Immunogens

Immunogen:
Uniprot:

O60500(NPHN_HUMAN) >>Visit HPA database.

Gene(ID):
NPHS1(4868)
Expression:
O60500 NPHN_HUMAN:

Specifically expressed in podocytes of kidney glomeruli.

Sequence:
MALGTTLRASLLLLGLLTEGLAQLAIPASVPRGFWALPENLTVVEGASVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACDLSDDAEYECQVGRSEMGPELVSPRVILSILVPPKLLLLTPEAGTMVTWVAGQEYVVNCVSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCEASSPALEAPIKASFTVNVLFPPGPPVIEWPGLDEGHVRAGQSLELPCVARGGNPLATLQWLKNGQPVSTAWGTEHTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHGITLQVTFPPSAIIILGSASQTENKNVTLSCVSKSSRPRVLLRWWLGWRQLLPMEETVMDGLHGGHISMSNLTFLARREDNGLTLTCEAFSEAFTKETFKKSLILNVKYPAQKLWIEGPPEGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESRLPQESRRVHLGSVEKSGSTFSRELVLVTGPSDNQAKFTCKAGQLSASTQLAVQFPPTNVTILANASALRPGDALNLTCVSVSSNPPVNLSWDKEGERLEGVAAPPRRAPFKGSAAARSVLLQVSSRDHGQRVTCRAHSAELRETVSSFYRLNVLYRPEFLGEQVLVVTAVEQGEALLPVSVSANPAPEAFNWTFRGYRLSPAGGPRHRILSSGALHLWNVTRADDGLYQLHCQNSEGTAEARLRLDVHYAPTIRALQDPTEVNVGGSVDIVCTVDANPILPGMFNWERLGEDEEDQSLDDMEKISRGPTGRLRIHHAKLAQAGAYQCIVDNGVAPPARRLLRLVVRFAPQVEHPTPLTKVAAAGDSTSSATLHCRARGVPNIVFTWTKNGVPLDLQDPRYTEHTYHQGGVHSSLLTIANVSAAQDYALFTCTATNALGSDQTNIQLVSISRPDPPSGLKVVSLTPHSVGLEWKPGFDGGLPQRFCIRYEALGTPGFHYVDVVPPQATTFTLTGLQPSTRYRVWLLASNALGDSGLADKGTQLPITTPGLHQPSGEPEDQLPTEPPSGPSGLPLLPVLFALGGLLLLSNASCVGGVLWQRRLRRLAEGISEKTEAGSEEDRVRNEYEESQWTGERDTQSSTVSTTEAEPYYRSLRDFSPQLPPTQEEVSYSRGFTGEDEDMAFPGHLYDEVERTYPPSGAWGPLYDEVQMGPWDLHWPEDTYQDPRGIYDQVAGDLDTLEPDSLPFELRGHLV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Zebrafish
100
Pig
90
Bovine
80
Sheep
80
Rabbit
80
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O60500 As Substrate

Site PTM Type Enzyme
T414 Phosphorylation
T416 Phosphorylation
S421 Phosphorylation
S432 Phosphorylation
Y439 Phosphorylation
Y638 Phosphorylation
S700 Phosphorylation
S701 Phosphorylation
T710 Phosphorylation
T1120 Phosphorylation P17252 (PRKCA)
T1125 Phosphorylation P17252 (PRKCA)
Y1176 Phosphorylation
Y1193 Phosphorylation P06241 (FYN)
Y1217 Phosphorylation

Research Backgrounds

Function:

Seems to play a role in the development or function of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slit diaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).

PTMs:

Phosphorylated at Tyr-1193 by FYN, leading to the recruitment and activation of phospholipase C-gamma-1/PLCG1.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.
Note: Predominantly located at podocyte slit diaphragm between podocyte foot processes. Also associated with podocyte apical plasma membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Specifically expressed in podocytes of kidney glomeruli.

Subunit Structure:

Interacts with CD2AP (via C-terminal domain). Interacts with MAGI1 (via PDZ 2 and 3 domains) forming a tripartite complex with IGSF5/JAM4. Interacts with DDN; the interaction is direct. Self-associates (via the Ig-like domains). Also interacts (via the Ig-like domains) with KIRREL1/NEPH1 and KIRREL2; the interaction with KIRREL1 is dependent on KIRREL1 glycosylation. Interacts with KIRREL3. Forms a complex with ACTN4, CASK, IQGAP1, MAGI2, SPTAN1 and SPTBN1 (By similarity). Interacts with NPHS2.

Family&Domains:

Belongs to the immunoglobulin superfamily.

References

1). C‐reactive protein inhibits C3a/C3aR‐dependent podocyte autophagy in favor of diabetic kidney disease. The FASEB Journal, 2022 (PubMed: 35503088) [IF=4.8]

Application: IHC    Species: Rat    Sample: glomeruli

FIGURE 5Podocyte injury and C3aR expression were evaluated in STZ-diabetic DKD rats. (A) Representative images of nephrin and WT1 expressed in glomeruli of DKD rats by IHC staining (n = 4). (B) Representative images of C3aR expressed in glomeruli of DKD rats by IHC staining (n = 4). (C and D) Quantitative analysis of nephrin, WT1 and C3aR was performed using Image-Pro Plus 6.0 software (n = 4, more than 20 glomeruli). (E and F) The colocalization of nephrin and C3aR in human renal biopsies and DKD kidney tissues by a commercial double staining kit. 400× original magnification; scale bar, 50 µm. Data are presented as the means ± SEM, and p < .05 was considered to be statistically significant
2). ADAM metallopeptidase domain 10 knockdown enables podocytes to resist high glucose stimulation by inhibiting pyroptosis via MAPK pathway. Experimental and Therapeutic Medicine, 2023 (PubMed: 37153901) [IF=2.7]

Application: WB    Species: Mouse    Sample:

Figure 2 ADAM10 knockdown inhibits HG-induced podocyte apoptosis. (A) Podocyte markers were evaluated using western blot analysis. (B) Podocyte apoptosis was evaluated using the TUNEL assay. (C) Apoptotic rate was obtained according to the results of the TUNEL assay. (D) Expression levels of proteins related to apoptosis were assessed using western blot analysis. ***P
3). Sericin suppresses high glucose-induced EMT in mouse podocytes via miR-30a-5p and its target Snai1. Pakistan journal of pharmaceutical sciences, 2023 (PubMed: 37869922) [IF=0.8]

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