Product: GSC2 Antibody
Catalog: DF3018
Description: Rabbit polyclonal antibody to GSC2
Application: WB IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Chicken
Mol.Wt.: 25 KD; 22kD(Calculated).
Uniprot: O15499
RRID: AB_2840997

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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
GSC2 Antibody detects endogenous levels of total GSC2.
RRID:
AB_2840997
Cite Format: Affinity Biosciences Cat# DF3018, RRID:AB_2840997.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GSC 2; GSCL; Homeobox protein goosecoid 2; Homeobox protein goosecoid like;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O15499 GSC2_HUMAN:

Detected in adult testis and pituitary, and in 9-10 week fetal tissue (thorax). Probably expressed in other tissues at low levels.

Sequence:
MAAAAGGAASRRGAGRPCPFSIEHILSSLPERSLPARAACPPQPAGRQSPAKPEEPGAPEAAPCACCCCCGPRAAPCGPPEAAAGLGARLAWPLRLGPAVPLSLGAPAGGSGALPGAVGPGSQRRTRRHRTIFSEEQLQALEALFVQNQYPDVSTRERLAGRIRLREERVEVWFKNRRAKWRHQKRASASARLLPGVKKSPKGSC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Chicken
82
Horse
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15499 As Substrate

Site PTM Type Enzyme
S122 Phosphorylation
S154 Phosphorylation
T155 Phosphorylation
S190 Phosphorylation

Research Backgrounds

Function:

May have a role in development. May regulate its own transcription. May bind the bicoid consensus sequence TAATCC.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in adult testis and pituitary, and in 9-10 week fetal tissue (thorax). Probably expressed in other tissues at low levels.

Family&Domains:

Belongs to the paired homeobox family. Bicoid subfamily.

Restrictive clause

 

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For Research Use Only.
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