Product: Cytochrome P450 2B6 Antibody
Catalog: AF0637
Source: Rabbit
Application: WB, IHC, IF/ICC, ELISA(peptide)
Reactivity: Human, Monkey
Mol.Wt.: 56kD; 56kD(Calculated).
Uniprot: P20813
RRID: AB_2834379

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200, ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Monkey
Clonality:
Polyclonal
Specificity:
Cytochrome P450 2B6 Antibody detects endogenous levels of total Cytochrome P450 2B6.
RRID:
AB_2834379
Cite Format: Affinity Biosciences Cat# AF0637, RRID:AB_2834379.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1,4 cineole 2 exo monooxygenase; 11B1; CP2B6_HUMAN; CPB 6; CPB6; CYP 2B6; CYP IIB6; CYP2B; CYP2B6; CYP2B7; CYP2B7P; CYPIIB6; Cytochrome P450 2B6; Cytochrome P450 family 2 subfamily B; Cytochrome P450 family 2 subfamily B polypeptide 6; Cytochrome P450 IIB1; Cytochrome P450 phenobarbital inducible; Cytochrome P450 subfamily IIB (phenobarbital inducible); Cytochrome P450 subfamily IIB (phenobarbital inducible) polypeptide 6; Cytochrome P450 subfamily IIB polypeptide 6; EFVM; IIB1; P450; P450 IIB1; P450IIB1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P20813 CP2B6_HUMAN:

Expressed in liver, lung and heart right ventricle.

Description:
This gene, CYP2B6, encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by phenobarbital. The enzyme is known to metabolize some xenobiotics, such as the anti-cancer drugs cyclophosphamide and ifosphamide. Transcript variants for this gene have been described; however, it has not been resolved whether these transcripts are in fact produced by this gene or by a closely related pseudogene, CYP2B7. Both the gene and the pseudogene are located in the middle of a CYP2A pseudogene found in a large cluster of cytochrome P450 genes from the CYP2A, CYP2B and CYP2F subfamilies on chromosome 19q.
Sequence:
MELSVLLFLALLTGLLLLLVQRHPNTHDRLPPGPRPLPLLGNLLQMDRRGLLKSFLRFREKYGDVFTVHLGPRPVVMLCGVEAIREALVDKAEAFSGRGKIAMVDPFFRGYGVIFANGNRWKVLRRFSVTTMRDFGMGKRSVEERIQEEAQCLIEELRKSKGALMDPTFLFQSITANIICSIVFGKRFHYQDQEFLKMLNLFYQTFSLISSVFGQLFELFSGFLKYFPGAHRQVYKNLQEINAYIGHSVEKHRETLDPSAPKDLIDTYLLHMEKEKSNAHSEFSHQNLNLNTLSLFFAGTETTSTTLRYGFLLMLKYPHVAERVYREIEQVIGPHRPPELHDRAKMPYTEAVIYEIQRFSDLLPMGVPHIVTQHTSFRGYIIPKDTEVFLILSTALHDPHYFEKPDAFNPDHFLDANGALKKTEAFIPFSLGKRICLGEGIARAELFLFFTTILQNFSMASPVAPEDIDLTPQECGVGKIPPTYQIRFLPR

PTMs - P20813 As Substrate

Site PTM Type Enzyme
S128 Phosphorylation P17612 (PRKACA)
K433 Acetylation

Research Backgrounds

Function:

A cytochrome P450 monooxygenase involved in the metabolism of endocannabinoids and steroids. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the epoxidation of double bonds of arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling. Hydroxylates steroid hormones, including testosterone at C-16 and estrogens at C-2. Plays a role in the oxidative metabolism of xenobiotics, including plant lipids and drugs. Acts as a 1,4-cineole 2-exo-monooxygenase.

Allele 2B6*9: Has low affinity for anandamide and can only produce 11,12 EpETrE-EAs.

PTMs:

Phosphorylation is accompanied by a decrease in enzyme activity.

Subcellular Location:

Endoplasmic reticulum membrane>Peripheral membrane protein. Microsome membrane>Peripheral membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in liver, lung and heart right ventricle.

Family&Domains:

Belongs to the cytochrome P450 family.

Research Fields

· Metabolism > Lipid metabolism > Arachidonic acid metabolism.

· Metabolism > Metabolism of cofactors and vitamins > Retinol metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). Hu Q et al. Constitutive androstane receptor weakens the induction of panaxytriol on CYP3A4 by repressing the activation of pregnane X receptor. Biochem Pharmacol 2018 Nov 8 (PubMed: 30414935) [IF=6.100]

Application: WB    Species: human    Sample: HepG2 cells

Fig.2| Effect of PXT on CYP2B6 mRNA and protein expression in HepG2 cells.The effect of PXT on CYP2B6 mRNA and protein in HepG2 cells at a series of concentrations (5, 10, 20, 40, and 80 μM) for 24h was determined by real-time Q-PCR analysis (A) and Western blot analysis (B-C). The determination of mRNA and protein was normalized to GAPDH and β-actin, respectively. CITCO (10 μM) and 0.1% DMSO were used as the positive and negative controls, respectively. Results are presented as fold induction relative to control, and all data are expressed mean ± SD of triplicate independent experiments.

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