PDLIM1 Antibody - #DF3003

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Product: PDLIM1 Antibody
Catalog: DF3003
Description: Rabbit polyclonal antibody to PDLIM1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 36 KD; 36kD(Calculated).
Uniprot: O00151
RRID: AB_2840982

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:200-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
PDLIM1 Antibody detects endogenous levels of total PDLIM1.
RRID:
AB_2840982
Cite Format: Affinity Biosciences Cat# DF3003, RRID:AB_2840982.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

C terminal LIM domain protein 1; C-terminal LIM domain protein 1; Carboxy terminal LIM domain protein 1; CLIM 1; CLIM1; CLP 36; CLP36; Elfin; Enigma; Enigma homolog; hCLIM 1; hCLIM1; LIM domain protein CLP 36; LIM domain protein CLP-36; LIM domain protein CLP36; PDLI1_HUMAN; PDLIM 1; Pdlim1; PDZ and LIM domain 1; PDZ and LIM domain protein 1;

Immunogens

Immunogen:
Uniprot:

O00151(PDLI1_HUMAN) >>Visit HPA database.

Gene(ID):
PDLIM1(9124)
Expression:
O00151 PDLI1_HUMAN:

Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.

Sequence:
MTTQQIDLQGPGPWGFRLVGGKDFEQPLAISRVTPGSKAALANLCIGDVITAIDGENTSNMTHLEAQNRIKGCTDNLTLTVARSEHKVWSPLVTEEGKRHPYKMNLASEPQEVLHIGSAHNRSAMPFTASPASSTTARVITNQYNNPAGLYSSENISNFNNALESKTAASGVEANSRPLDHAQPPSSLVIDKESEVYKMLQEKQELNEPPKQSTSFLVLQEILESEEKGDPNKPSGFRSVKAPVTKVAASIGNAQKLPMCDKCGTGIVGVFVKLRDRHRHPECYVCTDCGTNLKQKGHFFVEDQIYCEKHARERVTPPEGYEVVTVFPK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
91
Xenopus
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00151 As Substrate

Site PTM Type Enzyme
T2 Acetylation
K22 Acetylation
K22 Ubiquitination
S31 Phosphorylation
T34 Phosphorylation
K71 Ubiquitination
C73 S-Nitrosylation
K87 Methylation
K87 Ubiquitination
S90 Phosphorylation
T94 O-Glycosylation
K98 Acetylation
K98 Ubiquitination
S108 Phosphorylation
S118 Phosphorylation
S123 Phosphorylation
T128 Phosphorylation
S130 Phosphorylation
S133 Phosphorylation
S134 Phosphorylation
T136 Phosphorylation
T141 Phosphorylation
Y144 Phosphorylation
Y151 Phosphorylation
S152 Phosphorylation
S153 Phosphorylation
S186 Phosphorylation
S187 Phosphorylation
K192 Ubiquitination
S194 Phosphorylation
Y197 Phosphorylation
K198 Ubiquitination
K203 Ubiquitination
K211 Ubiquitination
S213 Phosphorylation
T214 Phosphorylation
S215 Phosphorylation
K233 Ubiquitination
S235 Phosphorylation
S239 Phosphorylation
K241 Ubiquitination
K246 Acetylation
K246 Ubiquitination
S250 Phosphorylation
K256 Ubiquitination
T291 Phosphorylation
Y306 Phosphorylation
K309 Ubiquitination
T316 Phosphorylation
Y321 Phosphorylation
T325 Phosphorylation

Research Backgrounds

Function:

Cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. Involved in assembly, disassembly and directioning of stress fibers in fibroblasts. Required for the localization of ACTN1 and PALLD to stress fibers. Required for cell migration and in maintaining cell polarity of fibroblasts (By similarity).

Subcellular Location:

Cytoplasm. Cytoplasm>Cytoskeleton. Cytoplasm>Myofibril>Sarcomere>Z line.
Note: Associates with actin stress fibers.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Strongly expressed in the heart and skeletal muscle, moderately expressed in the spleen, small intestine, colon, placenta, and lung. A lower level expression is seen in liver, thymus, kidney, prostate and pancreas and is not found in the brain, testis, ovary, and peripheral blood leukocytes.

Subunit Structure:

Interacts with ACTN1, ACTN2 and ACTN4. Interacts with PDLIM4 (By similarity).

References

1). Circulating proteomic panels for risk stratification of intracranial aneurysm and its rupture. EMBO Molecular Medicine, 2022 (PubMed: 34978375) [IF=11.1]

Application: WB    Species: Human    Sample: IA tissue and STA tissue

Figure 2 IA tissue proteome remodeling due to IA formation and rupture Five pairs of IA and STA tissues surgically excised from intracranial aneurysm patients. Differentially expressed proteins between IA group and STA group, as determined by plotting Student’s t‐test (P‐value < 0.05, two‐sided) P‐values versus the log2 fold change (IA/STA) are represented on volcano plots. Proteins with significant change in expression levels are indicated by pink (upregulated) and blue (downregulated) dots. The dashed line (x = ±1) represents the cutoff of the log2 fold change in protein levels, and dashed line (y = 2) represents the cutoff of the −log10 t‐test P‐value. Significantly altered top ranked pathways in IA tissues compared with STA tissues. Interaction network of tissue downregulated proteins in IA group compared to STA group. Western blot validation of selected protein (PDLIM1) differentially expressed between STA and IA. Downregulation of PDLIM1 in IA tissue as compared to STA tissue was confirmed. Immunoblots showing expression differences of PDLIM1 in STA and IA specimens are shown in the left panel using β‐tubulin as the loading control. The semi‐quantitative densitometric measurement of blot bands is summarized in the lower panel. Error bars indicate standard deviations (three biological replicates for each group, two‐tailed Student’s t‐test, * P‐value < 0.05).

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