Rab9 Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using Rab9 Antibody.

DF2957 at 1/100 staining Human pancreatic cancer by IHC-P.

Product:	Rab9 Antibody
Catalog:	DF2957
Description:	Rabbit polyclonal antibody to Rab9
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.:	23kDa; 23kD(Calculated).
Uniprot:	P51151
RRID:	AB_2840941

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

Rab9 Antibody detects endogenous levels of total Rab9.

RRID:

AB_2840941
Cite Format: Affinity Biosciences Cat# DF2957, RRID:AB_2840941.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2410064E05Rik; AI195561; DmRab9; Rab 9; RAB 9A; RAB9 member RAS oncogene family; RAB9A; RAB9A member RAS oncogene family; RAB9A_HUMAN; RAS ASSOCIATED PROTEIN RAB9; Ras related protein Rab 9A; Ras-related protein Rab-9A; Sid6061p; Sid99;

Immunogens

Immunogen:

Uniprot:

P51151(RAB9A_HUMAN) >>Visit HPA database.

Gene(ID):

RAB9A(9367)

Sequence:

P51151 RAB9A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGKSSLFKVILLGDGGVGKSSLMNRYVTNKFDTQLFHTIGVEFLNKDLEVDGHFVTMQIWDTAGQERFRSLRTPFYRGSDCCLLTFSVDDSQSFQNLSNWKKEFIYYADVKEPESFPFVILGNKIDISERQVSTEEAQAWCRDNGDYPYFETSAKDATNVAAAFEEAVRRVLATEDRSDHLIQTDTVNLHRKPKPSSSCC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Xenopus

Zebrafish

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51151 As Substrate

P51151

Site	PTM Type	Source
A2	Acetylation	Uniprot
K4	Ubiquitination	Uniprot
S5	Phosphorylation	Uniprot
S6	Phosphorylation	Uniprot
K20	Ubiquitination	Uniprot
T39	Phosphorylation	Uniprot
K103	Acetylation	Uniprot
K103	Ubiquitination	Uniprot
Y107	Phosphorylation	Uniprot
Y108	Phosphorylation	Uniprot
K125	Ubiquitination	Uniprot
K156	Ubiquitination	Uniprot
T175	Phosphorylation	Uniprot
S179	Phosphorylation	Uniprot
T187	Phosphorylation	Uniprot

Research Backgrounds

P51151_RAB9A_HUMAN

Function:

Involved in the transport of proteins between the endosomes and the trans Golgi network. Involved in the recruitment of SGSM2 to melanosomes and is required for the proper trafficking of melanogenic enzymes TYR, TYRP1 and DCT/TYRP2 to melanosomes in melanocytes.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side. Endoplasmic reticulum membrane. Golgi apparatus membrane. Late endosome. Cytoplasmic vesicle>Phagosome membrane>Lipid-anchor>Cytoplasmic side. Cytoplasmic vesicle>Phagosome. Cytoplasmic vesicle membrane. Melanosome.
Note: Colocalizes with OSBPL1A at the late endosome (PubMed:16176980). Recruited to phagosomes containing S.aureus or M.tuberculosis (PubMed:21255211).

Subunit Structure:

Interacts (preferentially in its GTP-bound form) with GCC2 (via its GRIP domain). Interacts (GTP-bound form) with SGSM1; the GDP-bound form has much lower affinity for SGSM1. Interacts with SGSM2. The GTP-bound form but not the GDP-bound form interacts with HPS4 and BLOC-3 complex (heterodimer of HPS1 and HPS4) but does not interact with HPS1 alone.

Family&Domains:

Belongs to the small GTPase superfamily. Rab family.

Research Fields

· Human Diseases > Infectious diseases: Viral > Measles.

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Rab9 Antibody - #DF2957