COX17 Antibody - #AF0626

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Product: COX17 Antibody
Catalog: AF0626
Description: Rabbit polyclonal antibody to COX17
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Dog, Xenopus
Mol.Wt.: 7kDa; 7kD(Calculated).
Uniprot: Q14061
RRID: AB_2834374

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 100ul $280 In stock
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Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(91%), Dog(82%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
COX17 Antibody detects endogenous levels of total COX17.
RRID:
AB_2834374
Cite Format: Affinity Biosciences Cat# AF0626, RRID:AB_2834374.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

COX 17; COX17; COX17 cytochrome c oxidase assembly homolog (S. cerevisiae); COX17 cytochrome c oxidase assembly homolog; COX17 homolog cytochrome c oxidase assembly protein; COX17_HUMAN; cytochrome c oxidase assembly protein cox17 homolog; Cytochrome c oxidase copper chaperone; Human homolog of yeast mitochondrial copper recruitment; MGC104397; MGC117386; OTTHUMP00000215284; OTTHUMP00000215285;

Immunogens

Immunogen:
Uniprot:

Q14061(COX17_HUMAN) >>Visit HPA database.

Gene(ID):
COX17(10063)
Expression:
Q14061 COX17_HUMAN:

Ubiquitous.

Description:
Cytochrome c oxidase (COX), the terminal component of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. This component is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may function in the regulation and assembly of the complex. This nuclear gene encodes a protein which is not a structural subunit, but may be involved in the recruitment of copper to mitochondria for incorporation into the COX apoenzyme. This protein shares 92% amino acid sequence identity with mouse and rat Cox17 proteins. This gene is no longer considered to be a candidate gene for COX deficiency. A pseudogene COX17P has been found on chromosome 13.
Sequence:
MPGLVDSNPAPPESQEKKPLKPCCACPETKKARDACIIEKGEEHCGHLIEAHKECMRALGFKI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Xenopus
100
Bovine
91
Dog
82
Rabbit
73
Horse
0
Sheep
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14061 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation
S14 Phosphorylation
K17 Acetylation
K18 Acetylation
K21 Acetylation
K30 Acetylation
K31 Acetylation
K62 Ubiquitination

Research Backgrounds

Function:

Copper metallochaperone essential for the assembly of the mitochondrial respiratory chain complex IV (CIV), also known as cytochrome c oxidase. Binds two copper ions and delivers them to the metallochaperone SCO1 which transports the copper ions to the Cu(A) site on the cytochrome c oxidase subunit II (MT-CO2/COX2).

Subcellular Location:

Mitochondrion intermembrane space. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with COA1. Interacts with the chaperone CHCHD4; this is important for correct folding and the formation of disulfide bonds that stabilize the structure.

Family&Domains:

Belongs to the COX17 family.

Research Fields

· Metabolism > Energy metabolism > Oxidative phosphorylation.

· Metabolism > Global and overview maps > Metabolic pathways.

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