HIF-2-alpha Antibody - #DF2928

Figure 7 Ferroptosis was induced in active UC patients with high-fat diet (A) Flow diagram. The correlation analysis between (B) Mayo score, (C) Fecal calcaretin, (D) Erythrocyte sedimentation rate (ESR) and dietary fats intake (%) in active UC patients with HFD. (E) Representative colonoscopy images of mice in active normal diet UC group and HFD_UC group. Representative images of HIF-2α, DMT1 and GPX4 immunohistochemical staining of colonic tissues (scale bars, 50 μm) from active UC and HFD-UC patients. (F) HIF-2α staining intensity in active UC and HFD-UC patients (n = 3 in each group). (G) DMT1 staining intensity in active UC and HFD-UC patients (n = 3 in each group). (H) GPX4 staining intensity in active UC and HFD-UC patients (n = 3 in each group). (I) The relative mRNA expression of GPX4 in health control tissues (n = 8) and active UC tissues (n = 7) indicated by data from GSE48958 dataset. (J) The relative mRNA expression of ACSL4 in health control tissues (n = 27) and active UC tissues (n = 7) indicated by data from GSE97012 dataset.

Figure 4 Deoxycholic acid activated HIF-2α/DMT1 signaling pathway (A–E) C57BL/6J mice were fed for 7 days in the following 4 conditions (n = 6 in each group): (1) drinking water and distilled water enema, control group, (2) drinking water and DCA enema, DCA group, (3) drinking water containing 2% DSS and distilled water enema, DSS group, (4) drinking water containing 2% DSS and DCA enema, DSS + DCA group. (A) Volcano-plots showing gene expression changes in transcription and epigenetics associated genes following DCA treatment (n = 3 in each group). (B) Hierarchical clustering of the gene expression values of the sub-set of genes that were differentially expressed between mice in DSS and DSS + DCA group (n = 3 in each group). Gene expression across rows in the heatmap were colored according to the z-score, such that the mean expression of each row was set to white color and expression values higher or lower than the mean was graded towards red or blue, respectively. (C) The Fragments Per Kilobase of transcript per Million mapped reads (FPKM) value of DMT1 in DSS and DSS + DCA group (n = 3 in each group). (D) The colonic mRNA expression of HIF-2α and DMT1 in mice from each group (n = 6 in each group). (E) Representative images of HIF-2α and DMT1 immunohistochemical staining (scale bars, 50 μm) of colonic tissues from each group (left panel). The relative staining intensity of HIF-2α and DMT1 from each group (right panel) (n = 3 in each group). (F-H) IEC-6 cells and Caco-2 cells were incubated with or without 10 ng/mL LPS for 12 h, followed by 200 μM DCA for 2 h. They were divided into four group (n = 6 in each group): control group, DCA group, LPS group and LPS + DCA group. (F) The relative mRNA expression of HIF-2α and DMT1 in each group of IEC-6 cells (n = 6 in each group). (G) The relative mRNA expression of HIF-2α and DMT1 in each group of Caco-2 cells (n = 6 in each group). (H) The protein level of HIF-2α, DMT1 and β-actin in each group of IEC-6 cells (left panel). Relative intensity of HIF-2α and DMT1 normalized to β-actin in each group was shown as the graph (right panel) (n = 3 in each group). Data are represented as mean ± SEM. ns P > 0.05, ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001, ∗∗∗∗P < 0.0001.