ITGAM Antibody | Affinity Biosciences

Product:	ITGAM Antibody
Catalog:	DF2911
Description:	Rabbit polyclonal antibody to ITGAM
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	170 kDa; 127kD(Calculated).
Uniprot:	P11215
RRID:	AB_2840900

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(88%), Horse(86%), Sheep(88%), Rabbit(88%), Dog(88%)

Clonality:

Polyclonal

Specificity:

ITGAM Antibody detects endogenous levels of total ITGAM.

RRID:

AB_2840900
Cite Format: Affinity Biosciences Cat# DF2911, RRID:AB_2840900.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

antigen CD11b (p170); Antigen CD11b p170; CD11 antigen like family member B; CD11 antigen-like family member B; CD11b; CD11b/CD18; CD49d; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; Complement Component Receptor 3 Alpha; Complement receptor type 3, alpha subunit; CR 3 alpha chain (CR3A); CR 3 alpha chain; CR-3 alpha chain; CR3; CR3A; F730045J24Rik; Integrin Alpha M; Integrin alpha M chain; Integrin alpha-M; Integrin beta 2 alpha subunit; Integrin subunit alpha M; integrin, alpha M (complement component 3 receptor 3 subunit); ITAM_HUMAN; ITGAM; Leukocyte adhesion receptor MO1; Ly-40; MAC 1; Mac-1a; MAC1; Mac1, alpha subunit; MAC1A; Macrophage antigen alpha polypeptide; MGC117044; Mo1, alpha subunit; MO1A; Neutrophil adherence receptor alpha M subunit; Neutrophil adherence receptor; SLEB6;

Immunogens

Immunogen:

Uniprot:

P11215(ITAM_HUMAN) >>Visit HPA database.

Gene(ID):

ITGAM(3684)

Expression:

P11215 ITAM_HUMAN:

Predominantly expressed in monocytes and granulocytes (PubMed:1346576). Expressed in neutrophils (at protein level) (PubMed:21193407).

Sequence:

P11215 ITAM_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALRVLLLTALTLCHGFNLDTENAMTFQENARGFGQSVVQLQGSRVVVGAPQEIVAANQRGSLYQCDYSTGSCEPIRLQVPVEAVNMSLGLSLAATTSPPQLLACGPTVHQTCSENTYVKGLCFLFGSNLRQQPQKFPEALRGCPQEDSDIAFLIDGSGSIIPHDFRRMKEFVSTVMEQLKKSKTLFSLMQYSEEFRIHFTFKEFQNNPNPRSLVKPITQLLGRTHTATGIRKVVRELFNITNGARKNAFKILVVITDGEKFGDPLGYEDVIPEADREGVIRYVIGVGDAFRSEKSRQELNTIASKPPRDHVFQVNNFEALKTIQNQLREKIFAIEGTQTGSSSSFEHEMSQEGFSAAITSNGPLLSTVGSYDWAGGVFLYTSKEKSTFINMTRVDSDMNDAYLGYAAAIILRNRVQSLVLGAPRYQHIGLVAMFRQNTGMWESNANVKGTQIGAYFGASLCSVDVDSNGSTDLVLIGAPHYYEQTRGGQVSVCPLPRGRARWQCDAVLYGEQGQPWGRFGAALTVLGDVNGDKLTDVAIGAPGEEDNRGAVYLFHGTSGSGISPSHSQRIAGSKLSPRLQYFGQSLSGGQDLTMDGLVDLTVGAQGHVLLLRSQPVLRVKAIMEFNPREVARNVFECNDQVVKGKEAGEVRVCLHVQKSTRDRLREGQIQSVVTYDLALDSGRPHSRAVFNETKNSTRRQTQVLGLTQTCETLKLQLPNCIEDPVSPIVLRLNFSLVGTPLSAFGNLRPVLAEDAQRLFTALFPFEKNCGNDNICQDDLSITFSFMSLDCLVVGGPREFNVTVTVRNDGEDSYRTQVTFFFPLDLSYRKVSTLQNQRSQRSWRLACESASSTEVSGALKSTSCSINHPIFPENSEVTFNITFDVDSKASLGNKLLLKANVTSENNMPRTNKTEFQLELPVKYAVYMVVTSHGVSTKYLNFTASENTSRVMQHQYQVSNLGQRSLPISLVFLVPVRLNQTVIWDRPQVTFSENLSSTCHTKERLPSHSDFLAELRKAPVVNCSIAVCQRIQCDIPFFGIQEEFNATLKGNLSFDWYIKTSHNHLLIVSTAEILFNDSVFTLLPGQGAFVRSQTETKVEPFEVPNPLPLIVGSSVGGLLLLALITAALYKLGFFKRQYKDMMSEGGPPGAEPQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Sheep

Dog

Rabbit

Horse

Pig

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11215 As Substrate

P11215

Site	PTM Type	Source
S574	Phosphorylation	Uniprot
S577	Phosphorylation	Uniprot
Y676	Phosphorylation	Uniprot
T805	Phosphorylation	Uniprot
N900	N-Glycosylation	Uniprot
T930	Phosphorylation	Uniprot
S931	Phosphorylation	Uniprot
N940	N-Glycosylation	Uniprot
N946	N-Glycosylation	Uniprot
S1142	Phosphorylation	Uniprot

Research Backgrounds

P11215_ITAM_HUMAN

Function:

Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils. May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity). May play a role in mast cell development (By similarity). Required with TYROBP/DAP12 in microglia to control production of microglial superoxide ions which promote the neuronal apoptosis that occurs during brain development (By similarity).

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Membrane raft>Single-pass type I membrane protein.

Tissue Specificity:

Predominantly expressed in monocytes and granulocytes. Expressed in neutrophils (at protein level).

Subunit Structure:

Heterodimer of an alpha and a beta subunit. ITGAM associates with ITGB2. Found in a complex with CD177 and ITGB2/CD18. Interacts with JAM3. Interacts with THBD. Interacts with complement factor H/CFH; this interaction mediates adhesion of neutrophils to pathogens leading to pathogen clearance.

Family&Domains:

The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

Belongs to the integrin alpha chain family.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton. (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs). (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration. (View pathway)

References

1). Combination of resolvin E1 and lipoxin A4 promotes the resolution of pulpitis by inhibiting NF-κB activation through upregulating sirtuin 7 in dental pulp fibroblasts. CELL PROLIFERATION, 2022 (PubMed: 35411569) [IF=8.5]

Application: IF/ICC Species: rat Sample: Dental pulp fibroblasts

FIGURE 5|Therapeutic effect of combined administration of RvE1 and LXA4 in a rat pulpitis model. (A) Representative haematoxylin–eosinstained images on days 1, 3 and 7 after surgery. (B) Representative immunofluorescence images of CD11b on days 1, 3 and 7 after surgery.

2). Comprehensive Analysis of HHLA2 as a Prognostic Biomarker and Its Association With Immune Infiltrates in Hepatocellular Carcinoma. Frontiers in Immunology, 2022 (PubMed: 35371079) [IF=7.3]

3). Focal ischemic stroke modifies microglia-derived exosomal miRNAs: potential role of mir-212-5p in neuronal protection and functional recovery. Biological Research, 2023 (PubMed: 37789455) [IF=6.7]

Application: WB Species: Rat Sample:

Fig. 2 MiRNA sequencing of microglial exosomes 3 days after MCAO/R and qRT–PCR validation. A, B Characterisation of microglial exosomes using nanoparticle tracking analysis and transmission electron microscopy scanning. Scale bar = 200 nm. C The exosome markers CD9, CD63, and CD81 were detected using western blot analysis. D Microglia-derived exosomes were detected by western blot analysis using the microglia marker CD11b. E Heatmap showing the levels of miRNAs in microglial exosomes at 3 days after MCAO/R. F Expression of miR-30c-5p, miR-126a-5p, miR-128-3p, miR-212-5p and miR-1949 in the ischemic penumbra of the cortex at 3 days after MCAO/R was determined using qRT–PCR. G Expression levels of the miRNA-targeting genes PLXNA2, PTEN and FOXO3 in the ischemic penumbra of the cortex at 3 days after MCAO/R. H The target sites of miR-212-5p in PLXNA2 mRNA 3’ untranslated region (3’UTR). I Map of the pmirGLO luciferase reporter vector. J Dual luciferase assays revealed the binding of miR-212-5p to the 3’UTR of PLXNA2. The data are presented as the means ± SEM (n = 5 per group). *P

4). Inhibition of NLRP3 inflammasome activation and pyroptosis with the ethyl acetate fraction of Bungeanum ameliorated cognitive dysfunction in aged mice. Food & Function, 2021 (PubMed: 34231604) [IF=6.1]

5). The Role of Bone Morphogenetic Protein 4 in Microglial Polarization in the Process of Neuropathic Pain. Journal of Inflammation Research, 2022 (PubMed: 35535051) [IF=4.5]

6). Infiltration of Blood-Derived Macrophages Contributes to the Development of Diabetic Neuropathy. Journal of Immunology Research, 2019 (PubMed: 31534976) [IF=4.1]

7). Surfactant protein A is expressed in the central nervous system of rats with experimental autoimmune encephalomyelitis, and suppresses inflammation in human astrocytes and microglia. Molecular Medicine Reports, 2017 (PubMed: 28393255) [IF=3.4]

Application: WB Species: rat Sample:

Figure 3. Western blot analysis of GFAP, Iba‑1, CD11b and SP‑A in the rat CNS at different stages of EAE. (A) Proteins obtained from the rat CNS at the initial, peak and recovery stages of EAE were detected for GFAP, IBA‑1, CD11b and SP‑A via western blotting. (B) Quantitative analysis of western blots was performed and levels were normalized relative to β‑actin. Results are presented as the mean ± standard deviation. * P

Application: IHC Species: rat Sample:

Figure 2. H&E staining and immunohistochemical staining of GFAP, Iba‑1, CD11b and SP‑A in the rat CNS at different stages of EAE. (A) CNS sections at initial, peak and recovery stages of EAE were stained for inflammation by H&E, GFAP‑positive astrocytes, Iba‑1‑postive microglia, CD11b general marker of inflammatory cells and SP‑A. Whole image magnification, x200 and corner image magnification, x400. Section of rats under normal conditions served as the control. Arrows indicate the location of SP‑A expressing cells. (B) Lu sections of normal rats were stained as a positive control. Negative control sections of normal rat CNS were treated with secondary antibody only and were unstained for GFAP, Iba‑1, CD11b and SP‑A. Magnification, x400. H&E, hematoxylin and eosin; GFAP, glial fibrillary acidic protein; Iba‑1, ionized calcium‑binding adapter molecule 1; SP‑A, surfactant protein‑A; Lu, lung; CNS, central nervous system; EAE, experimental autoimmune encephalomyelitis.

8). Dexmedetomidine attenuates neuroinflammation and microglia activation in LPS-stimulated BV2 microglia cells through targeting circ-Shank3/miR-140-3p/TLR4 axis. European Journal of Histochemistry, 2023 (PubMed: 37491974) [IF=2.0]

Application: WB Species: Mouse Sample: BV2 cells

Figure 5. Dex attenuates LPS-induced microglia activation by modulating circ-Shank3/miR-140-3p axis. BV2 cells were treated with 20 μg/mL Dex plus miR-140-3p mimics, circ-Shank3 siRNA1 or circ-Shank3 siRNA1 + miR-140-3p inhibitor, followed by treatment with 10 μg/mL LPS. A-C) Immunofluorescence assay was used to estimate the expression of Iba-1, CD11b and BDNF in BV2 cells, based on the fluorescence intensity; Iba-1, CD11b or BDNF, red color; DAPI, blue color.

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ITGAM Antibody - #DF2911