Collagen XIV alpha 1 Antibody - #AF0573

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Product: Collagen XIV alpha 1 Antibody
Catalog: AF0573
Description: Rabbit polyclonal antibody to Collagen XIV alpha 1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Bovine, Rabbit, Dog, Xenopus
Mol.Wt.: 193kDa; 194kD(Calculated).
Uniprot: Q05707
RRID: AB_2834370

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Bovine(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
Collagen XIV alpha 1 Antibody detects endogenous levels of total Collagen XIV alpha 1.
RRID:
AB_2834370
Cite Format: Affinity Biosciences Cat# AF0573, RRID:AB_2834370.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Collagen alpha 1(XIV) chain; Collagen type XIV alpha 1 (undulin); Collagen type XIV alpha 1; UND; Undulin (fibronectin tenascin related); Undulin; COL14A1;

Immunogens

Immunogen:
Uniprot:

Q05707(COEA1_HUMAN) >>Visit HPA database.

Gene(ID):
COL14A1(7373)
Description:
COL14A1 Plays an adhesive role by integrating collagen bundles. It is probably associated with the surface of interstitial collagen fibrils via COL1. The COL2 domain may then serve as a rigid arm which sticks out from the fibril and protrudes the large N-terminal globular domain into the extracellular space, where it might interact with other matrix molecules or cell surface receptors. Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.
Sequence:
MKIFQRKMRYWLLPPFLAIVYFCTIVQGQVAPPTRLRYNVISHDSIQISWKAPRGKFGGYKLLVTPTSGGKTNQLNLQNTATKAIIQGLMPDQNYTVQIIAYNKDKESKPAQGQFRIKDLEKRKDPKPRVKVVDRGNGSRPSSPEEVKFVCQTPAIADIVILVDGSWSIGRFNFRLVRHFLENLVTAFDVGSEKTRIGLAQYSGDPRIEWHLNAFSTKDEVIEAVRNLPYKGGNTLTGLALNYIFENSFKPEAGSRTGVSKIGILITDGKSQDDIIPPSRNLRESGVELFAIGVKNADVNELQEIASEPDSTHVYNVAEFDLMHTVVESLTRTLCSRVEEQDREIKASAHAITGPPTELITSEVTARSFMVNWTHAPGNVEKYRVVYYPTRGGKPDEVVVDGTVSSTVLKNLMSLTEYQIAVFAIYAHTASEGLRGTETTLALPMASDLLLYDVTENSMRVKWDAVPGASGYLILYAPLTEGLAGDEKEMKIGETHTDIELSGLLPNTEYTVTVYAMFGEEASDPVTGQETTLALSPPRNLRISNVGSNSARLTWDPTSRQINGYRIVYNNADGTEINEVEVDPITTFPLKGLTPLTEYTIAIFSIYDEGQSEPLTGVFTTEEVPAQQYLEIDEVTTDSFRVTWHPLSADEGLHKLMWIPVYGGKTEEVVLKEEQDSHVIEGLEPGTEYEVSLLAVLDDGSESEVVTAVGTTLDSFWTEPATTIVPTTSVTSVFQTGIRNLVVGDETTSSLRVKWDISDSDVQQFRVTYMTAQGDPEEEVIGTVMVPGSQNNLLLKPLLPDTEYKVTVTPIYTDGEGVSVSAPGKTLPSSGPQNLRVSEEWYNRLRITWDPPSSPVKGYRIVYKPVSVPGPTLETFVGADINTILITNLLSGMDYNVKIFASQASGFSDALTGMVKTLFLGVTNLQAKHVEMTSLCAHWQVHRHATAYRVVIESLQDRQKQESTVGGGTTRHCFYGLQPDSEYKISVYTKLQEIEGPSVSIMEKTQSLPTRPPTFPPTIPPAKEVCKAAKADLVFMVDGSWSIGDENFNKIISFLYSTVGALNKIGTDGTQVAMVQFTDDPRTEFKLNAYKTKETLLDAIKHISYKGGNTKTGKAIKYVRDTLFTAESGTRRGIPKVIVVITDGRSQDDVNKISREMQLDGYSIFAIGVADADYSELVSIGSKPSARHVFFVDDFDAFKKIEDELITFVCETASATCPVVHKDGIDLAGFKMMEMFGLVEKDFSSVEGVSMEPGTFNVFPCYQLHKDALVSQPTRYLHPEGLPSDYTISFLFRILPDTPQEPFALWEILNKNSDPLVGVILDNGGKTLTYFNYDQSGDFQTVTFEGPEIRKIFYGSFHKLHIVVSETLVKVVIDCKQVGEKAMNASANITSDGVEVLGKMVRSRGPGGNSAPFQLQMFDIVCSTSWANTDKCCELPGLRDDESCPDLPHSCSCSETNEVALGPAGPPGGPGLRGPKGQQGEPGPKGPDGPRGEIGLPGPQGPPGPQGPSGLSIQGMPGMPGEKGEKGDTGLPGPQGIPGGVGSPGRDGSPGQRGLPGKDGSSGPPGPPGPIGIPGTPGVPGITGSMGPQGALGPPGVPGAKGERGERGDLQSQAMVRSVARQVCEQLIQSHMARYTAILNQIPSHSSSIRTVQGPPGEPGRPGSPGAPGEQGPPGTPGFPGNAGVPGTPGERGLTGIKGEKGNPGVGTQGPRGPPGPAGPSGESRPGSPGPPGSPGPRGPPGHLGVPGPQGPSGQPGYCDPSSCSAYGVRAPHPDQPEFTPVQDELEAMELWGPGV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
78
Pig
0
Horse
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q05707 As Substrate

Site PTM Type Enzyme
N94 N-Glycosylation
N372 N-Glycosylation
T439 Phosphorylation
T643 O-Glycosylation
S648 Phosphorylation
Y662 Phosphorylation
S963 Phosphorylation
T970 Phosphorylation
Y988 Phosphorylation
T989 Phosphorylation
T1067 Phosphorylation
T1070 Phosphorylation
T1078 Phosphorylation
Y1118 Phosphorylation
T1122 Phosphorylation
T1125 Phosphorylation
S1128 Phosphorylation
N1384 N-Glycosylation
N1388 N-Glycosylation
S1648 Phosphorylation
S1664 Phosphorylation
S1728 Phosphorylation
S1734 Phosphorylation
Y1758 Phosphorylation
Y1767 Phosphorylation

Research Backgrounds

Function:

Plays an adhesive role by integrating collagen bundles. It is probably associated with the surface of interstitial collagen fibrils via COL1. The COL2 domain may then serve as a rigid arm which sticks out from the fibril and protrudes the large N-terminal globular domain into the extracellular space, where it might interact with other matrix molecules or cell surface receptors (By similarity).

PTMs:

Lysines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in all cases and bind carbohydrates.

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.

May contain numerous cysteine residues involved in inter- and intramolecular disulfide bonding.

Subcellular Location:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homotrimer.

Family&Domains:

Belongs to the fibril-associated collagens with interrupted helices (FACIT) family.

Research Fields

· Organismal Systems > Digestive system > Protein digestion and absorption.

References

1). Whole-exome mutational landscape and molecular marker study in mucinous and clear cell ovarian cancer cell lines 3AO and ES2. BMC Cancer, 2023 [IF=3.8]

Application: WB    Species: Human    Sample:

Fig. 6 Differential expression of CCDC170, COL14A1 and THBS2 in ovarian cancer cell lines and normal ovarian epithelial cells. (A) Western blotting was used to detect the expression of CCDC170, COL14A1 and THBS2 in ovarian cancer cell lines and normal ovarian epithelial cells. In order to improve the clarity and conciseness of the presentation, we cropped the blots. The original, untreated blots images can be found in Additional file 6. (B-D) Statistical analysis of Western blotting results of CCDC170, COL14A1 and THBS2 proteins. (E-G) The expression of CCDC170, COL14A1 and THBS2 in ovarian cancer cell lines and normal ovarian epithelial cells was detected by immunofluorescence assay. Original magnification: 40×. (H-J) Statistical analysis of mean fluorescence density of CCDC170, COL14A1 and THBS2 proteins. The gray values of protein bands and the fluorescence intensity of immunofluorescence were analyzed using Image J software (National Institutes of Health, Bethesda, MD, USA).

Application: IF/ICC    Species: Human    Sample:

Fig. 6 Differential expression of CCDC170, COL14A1 and THBS2 in ovarian cancer cell lines and normal ovarian epithelial cells. (A) Western blotting was used to detect the expression of CCDC170, COL14A1 and THBS2 in ovarian cancer cell lines and normal ovarian epithelial cells. In order to improve the clarity and conciseness of the presentation, we cropped the blots. The original, untreated blots images can be found in Additional file 6. (B-D) Statistical analysis of Western blotting results of CCDC170, COL14A1 and THBS2 proteins. (E-G) The expression of CCDC170, COL14A1 and THBS2 in ovarian cancer cell lines and normal ovarian epithelial cells was detected by immunofluorescence assay. Original magnification: 40×. (H-J) Statistical analysis of mean fluorescence density of CCDC170, COL14A1 and THBS2 proteins. The gray values of protein bands and the fluorescence intensity of immunofluorescence were analyzed using Image J software (National Institutes of Health, Bethesda, MD, USA).
2). Identification of Underlying Hub Genes Associated with Hypertrophic Cardiomyopathy by Integrated Bioinformatics Analysis. Pharmacogenomics and Personalized Medicine, 2021 (PubMed: 34285551)

Application: WB    Species: Mouse    Sample: myocardium

Figure 8 Western blotting tests representing protein levels of hub genes (FRZB, COL14A1, CRISPLD1, LUM, sFRP4) and remodeling gene RCAN in myocardium of sham and TAC mice. Abbreviations: FRZB, frizzled related protein; COL14A1, collagen type XIV alpha 1 chain; CRISPLD1, cysteine rich secretory protein LCCL domain containing 1; LUM, lumican; sFRP4, secreted frizzled related protein 4; RCAN, calcipressin regulatory protein.

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