TAS1R1 Antibody | Affinity Biosciences

WB
Cites

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Western blot analysis of extracts from Rat brain, using TAS1R1 Antibody at 1/1000 dilution.

Figure 8 Transcription changes of umami tasting molecules, TAS1R1, TAS1R3 and PLCB2 during testis development of the Congjiang Xiang pig, evidenced by q-PCR.

Product:	TAS1R1 Antibody
Catalog:	DF10278
Description:	Rabbit polyclonal antibody to TAS1R1
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Dog, Chicken
Mol.Wt.:	93kDa; 93kD(Calculated).
Uniprot:	Q7RTX1
RRID:	AB_2840856

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(88%), Bovine(88%), Horse(88%), Sheep(88%), Dog(88%), Chicken(88%)

Clonality:

Polyclonal

Specificity:

TAS1R1 Antibody detects endogenous levels of total TAS1R1.

RRID:

AB_2840856
Cite Format: Affinity Biosciences Cat# DF10278, RRID:AB_2840856.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

G protein coupled receptor 70; G protein-coupled receptor 70; G-protein coupled receptor 70; gm 148; gm148; GPR 70; GPR70; Krueppel related zinc finger protein 3 (HKR3 protein); seven transmembrane helix receptor; Sweet taste receptor T1r; T1R1; TAS1 R1; TAS1R1; Taste receptor type 1 member 1; taste receptor, type 1, member 1; TR 1; TR1; TS1R1_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q7RTX1(TS1R1_HUMAN) >>Visit HPA database.

Gene(ID):

TAS1R1(80835)

Sequence:

Q7RTX1 TS1R1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MLLCTARLVGLQLLISCCWAFACHSTESSPDFTLPGDYLLAGLFPLHSGCLQVRHRPEVTLCDRSCSFNEHGYHLFQAMRLGVEEINNSTALLPNITLGYQLYDVCSDSANVYATLRVLSLPGQHHIELQGDLLHYSPTVLAVIGPDSTNRAATTAALLSPFLVPMISYAASSETLSVKRQYPSFLRTIPNDKYQVETMVLLLQKFGWTWISLVGSSDDYGQLGVQALENQATGQGICIAFKDIMPFSAQVGDERMQCLMRHLAQAGATVVVVFSSRQLARVFFESVVLTNLTGKVWVASEAWALSRHITGVPGIQRIGMVLGVAIQKRAVPGLKAFEEAYARADKKAPRPCHKGSWCSSNQLCRECQAFMAHTMPKLKAFSMSSAYNAYRAVYAVAHGLHQLLGCASGACSRGRVYPWQLLEQIHKVHFLLHKDTVAFNDNRDPLSSYNIIAWDWNGPKWTFTVLGSSTWSPVQLNINETKIQWHGKDNQVPKSVCSSDCLEGHQRVVTGFHHCCFECVPCGAGTFLNKSDLYRCQPCGKEEWAPEGSQTCFPRTVVFLALREHTSWVLLAANTLLLLLLLGTAGLFAWHLDTPVVRSAGGRLCFLMLGSLAAGSGSLYGFFGEPTRPACLLRQALFALGFTIFLSCLTVRSFQLIIIFKFSTKVPTFYHAWVQNHGAGLFVMISSAAQLLICLTWLVVWTPLPAREYQRFPHLVMLECTETNSLGFILAFLYNGLLSISAFACSYLGKDLPENYNEAKCVTFSLLFNFVSWIAFFTTASVYDGKYLPAANMMAGLSSLSSGFGGYFLPKCYVILCRPDLNSTEHFQASIQDYTRRCGST

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

Horse

Bovine

Sheep

Dog

Chicken

Rabbit

Zebrafish

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7RTX1 As Substrate

Q7RTX1

Site	PTM Type	Source
T198	Phosphorylation	Uniprot
T310	Phosphorylation	Uniprot
Y709	Phosphorylation	Uniprot
Y787	Phosphorylation	Uniprot

Research Backgrounds

Q7RTX1_TS1R1_HUMAN

Function:

Putative taste receptor. TAS1R1/TAS1R3 responds to the umami taste stimulus (the taste of monosodium glutamate). Sequence differences within and between species can significantly influence the selectivity and specificity of taste responses.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Subunit Structure:

Forms heterodimers with TAS1R3.

Family&Domains:

Belongs to the G-protein coupled receptor 3 family. TAS1R subfamily.

Research Fields

· Organismal Systems > Sensory system > Taste transduction.

References

1). Longitudinal Expression of Testicular TAS1R3 from Prepuberty to Sexual Maturity in Congjiang Xiang Pigs. Animals, 2021 (PubMed: 33567555) [IF=3.0]

Application: WB Species: Pigs Sample:

Figure 8 Transcription changes of umami tasting molecules, TAS1R1, TAS1R3 and PLCB2 during testis development of the Congjiang Xiang pig, evidenced by q-PCR. Gel electrophoresis q-PCR result of TAS1R1 (167 bp), TA1SR3 (235 bp), and PLCB2 (239 bp), respectively. The mRNA levels of target genes were normalized with housekeeping gene (ATCB, 61 bp) and expressed as 2−ΔΔCT using samples on day 30 as calibrator. Results are indicated by means ± SD (n = 3). Values not sharing the same letter (a, b) denote significant differences among groups (p < 0.05).

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TAS1R1 Antibody - #DF10278