P2RY12 Antibody - #DF10263

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Product: P2RY12 Antibody
Catalog: DF10263
Description: Rabbit polyclonal antibody to P2RY12
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Horse, Dog
Mol.Wt.: 39 kDa; 39kD(Calculated).
Uniprot: Q9H244
RRID: AB_2840841

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(86%), Dog(88%)
Clonality:
Polyclonal
Specificity:
P2RY12 Antibody detects endogenous levels of total P2RY12.
RRID:
AB_2840841
Cite Format: Affinity Biosciences Cat# DF10263, RRID:AB_2840841.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADP glucose receptor; ADP-glucose receptor; ADPG R; ADPG-R; ADPGR; BDPLT8; G protein coupled receptor SP1999; Gi coupled ADP receptor HORK 3; Gi coupled ADP receptor HORK3; HORK 3; HORK3; P2RY 12; P2RY12; P2T(AC); P2Y 12; P2Y purinoceptor 12; P2Y(12)R; P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; P2Y12 platelet ADP receptor; P2Y12_HUMAN; Platelet ADP receptor; Purinergic receptor P2RY12; Purinergic receptor P2Y G protein coupled 12; Purinergic receptor P2Y12; Putative G protein coupled receptor; SP 1999; SP1999;

Immunogens

Immunogen:
Uniprot:

Q9H244(P2Y12_HUMAN) >>Visit HPA database.

Gene(ID):
P2RY12(64805)
Expression:
Q9H244 P2Y12_HUMAN:

Highly expressed in the platelets, lower levels in the brain. Lowest levels in the lung, appendix, pituitary and adrenal gland. Expressed in the spinal cord and in the fetal brain.

Sequence:
MQAVDNLTSAPGNTSLCTRDYKITQVLFPLLYTVLFFVGLITNGLAMRIFFQIRSKSNFIIFLKNTVISDLLMILTFPFKILSDAKLGTGPLRTFVCQVTSVIFYFTMYISISFLGLITIDRYQKTTRPFKTSNPKNLLGAKILSVVIWAFMFLLSLPNMILTNRQPRDKNVKKCSFLKSEFGLVWHEIVNYICQVIFWINFLIVIVCYTLITKELYRSYVRTRGVGKVPRKKVNVKVFIIIAVFFICFVPFHFARIPYTLSQTRDVFDCTAENTLFYVKESTLWLTSLNACLDPFIYFFLCKSFRNSLISMLKCPNSATSLSQDNRKKEQDGGDPNEETPM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
88
Horse
86
Pig
78
Rabbit
67
Bovine
63
Sheep
63
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H244 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
T89 Phosphorylation
Y105 Phosphorylation
T107 Phosphorylation
S262 Phosphorylation
T264 Phosphorylation
S318 Phosphorylation

Research Backgrounds

Function:

Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Not activated by UDP and UTP. Required for normal platelet aggregation and blood coagulation.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in the platelets, lower levels in the brain. Lowest levels in the lung, appendix, pituitary and adrenal gland. Expressed in the spinal cord and in the fetal brain.

Family&Domains:

The transmembrane domain is composed of seven transmembrane helices; most of these are not strictly perpendicular to the plane of the membrane, but are tilted and/or kinked. Agonist binding promotes a conformation change in the extracellular loops that leads to an inward movement of the transmembrane helices. Antagonists such as AZD1283 can bind to an overlapping site, but block the inward movement of the transmembrane helices (PubMed:24670650, PubMed:24784220).

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

References

1). Huanglian Jiedu decoction inhibits vascular smooth muscle cell-derived foam cell formation by activating autophagy via suppressing P2RY12. Journal of ethnopharmacology, 2024 (PubMed: 38561055) [IF=5.4]

Application: WB    Species: Mouse    Sample:

Fig. 3. P2RY12 was involved in the alleviation of AS and foam cell formation by HLJDD. P2RY12 protein expression correlated with (a, n = 6) the incubation dose and (b, n = 5) the duration of oxLDL treatment. (c, n = 6) Its expression was decreased after HLJDD intervention. HFD-fed ApoE−/− mice underwent gavage with HLJDD and CDL. (d, n = 7) Body weight of mice at sacrifice. AS was evaluated using (e) ORO, (f) Nile Red, (g) HE, and (h) Sirius Red staining of aortic root sections. n = 3–4 per group. Scale bar: 200 μm (100 μm in local magnification). Data were presented as mean ± standard deviation in one-way ANOVA with Dunnett post hoc tests. ns: not significant
2). P2X7 receptor of microglia in olfactory bulb mediates the pathogenesis of olfactory dysfunction in a mouse model of allergic rhinitis. The FASEB Journal, 2023 [IF=4.8]
3). miR-6076 rs1463411 polymorphisms are associated with bleeding during clopidogrel treatment in patients with acute coronary syndrome. Clinical & Translational Oncology, 2023 (PubMed: 36795259) [IF=3.4]

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