TAF6L Antibody | Affinity Biosciences

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Western blot analysis on 293 cell lysates using TAF6L Antibody,The lane on the left was treated with the antigen-specific peptide.

Product:	TAF6L Antibody
Catalog:	AF0691
Description:	Rabbit polyclonal antibody to TAF6L
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	68kDa; 68kD(Calculated).
Uniprot:	Q9Y6J9
RRID:	AB_2834363

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

TAF6L Antibody detects endogenous levels of total TAF6L.

RRID:

AB_2834363
Cite Format: Affinity Biosciences Cat# AF0691, RRID:AB_2834363.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CBP associated factor associated factor 65 kDa subunit 6L; PAF65 alpha; PAF65A; PCAF associated factor 65 alpha; TAF6 like RNA polymerase II p300/CBP associated factor associated factor 65 kDa subunit 6L; TAF6-like RNA polymerase II p300;

Immunogens

Immunogen:

Uniprot:

Q9Y6J9(TAF6L_HUMAN) >>Visit HPA database.

Gene(ID):

TAF6L(10629)

Description:

TAF6L Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. Belongs to the TAF6 family. The PCAF complex is composed of a number of TBP- associated factors (TAFS), such as TAF5, TAF5L, TAF6, TAF6L, TAF9, TAF10 and TAF12, PCAF, and also PCAF-associated factors (PAFs), such as TADA2L/ADA2, TADA3L/ADA3 and SPT3. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9.

Sequence:

Q9Y6J9 TAF6L_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSEREERRFVEIPRESVRLMAESTGLELSDEVAALLAEDVCYRLREATQNSSQFMKHTKRRKLTVEDFNRALRWSSVEAVCGYGSQEALPMRPAREGELYFPEDREVNLVELALATNIPKGCAETAVRVHVSYLDGKGNLAPQGSVPSAVSSLTDDLLKYYHQVTRAVLGDDPQLMKVALQDLQTNSKIGALLPYFVYVVSGVKSVSHDLEQLHRLLQVARSLFRNPHLCLGPYVRCLVGSVLYCVLEPLAASINPLNDHWTLRDGAALLLSHIFWTHGDLVSGLYQHILLSLQKILADPVRPLCCHYGAVVGLHALGWKAVERVLYPHLSTYWTNLQAVLDDYSVSNAQVKADGHKVYGAILVAVERLLKMKAQAAEPNRGGPGGRGCRRLDDLPWDSLLFQESSSGGGAEPSFGSGLPLPPGGAGPEDPSLSVTLADIYRELYAFFGDSLATRFGTGQPAPTAPRPPGDKKEPAAAPDSVRKMPQLTASAIVSPHGDESPRGSGGGGPASASGPAASESRPLPRVHRARGAPRQQGPGTGTRDVFQKSRFAPRGAPHFRFIIAGRQAGRRCRGRLFQTAFPAPYGPSPASRYVQKLPMIGRTSRPARRWALSDYSLYLPL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Bovine

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6J9 As Substrate

Q9Y6J9

Site	PTM Type	Source
S16	Phosphorylation	Uniprot
Y42	Phosphorylation	Uniprot
K56	Ubiquitination	Uniprot
K62	Ubiquitination	Uniprot
S85	Phosphorylation	Uniprot
K120	Ubiquitination	Uniprot
K137	Ubiquitination	Uniprot
S145	Phosphorylation	Uniprot
K177	Ubiquitination	Uniprot
Y327	Phosphorylation	Uniprot
Y333	Phosphorylation	Uniprot
T335	Phosphorylation	Uniprot
Y344	Phosphorylation	Uniprot
K373	Ubiquitination	Uniprot
T458	Phosphorylation	Uniprot
T464	Phosphorylation	Uniprot
S481	Phosphorylation	Uniprot
S491	Phosphorylation	Uniprot
S495	Phosphorylation	Uniprot
S501	Phosphorylation	Uniprot
K549	Ubiquitination	Uniprot
R555	Methylation	Uniprot
R561	Methylation	Uniprot
R567	Methylation	Uniprot
S589	Phosphorylation	Uniprot
R593	Methylation	Uniprot
K597	Ubiquitination	Uniprot

Research Backgrounds

Q9Y6J9_TAF6L_HUMAN

Function:

Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex (Probable). With TAF5L, acts as an epigenetic regulator essential for somatic reprogramming. Regulates target genes through H3K9ac deposition and MYC recruitment which trigger MYC regulatory network to orchestrate gene expression programs to control embryonic stem cell state. Functions with MYC to activate target gene expression through RNA polymerase II pause release (By similarity).

Subcellular Location:

Nucleus.

Subunit Structure:

The PCAF complex is composed of a number of TBP-associated factors (TAFS), such as TAF5, TAF5L, TAF6, TAF6L, TAF9, TAF10 and TAF12, PCAF, and also PCAF-associated factors (PAFs), such as TADA2L/ADA2, TADA3L/ADA3 and SPT3. Component of the STAGA transcription coactivator-HAT complex, at least composed of SUPT3H, GCN5L2, TAF5L, TAF6L, SUPT7L, TADA3L, TAD1L, TAF10, TAF12, TRRAP and TAF9.

Family&Domains:

Belongs to the TAF6 family.

Research Fields

· Genetic Information Processing > Transcription > Basal transcription factors.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

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TAF6L Antibody - #AF0691