ATG4B Antibody - #AF0425

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Product: ATG4B Antibody
Catalog: AF0425
Description: Rabbit polyclonal antibody to ATG4B
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Rabbit, Dog
Mol.Wt.: 44kDa; 44kD(Calculated).
Uniprot: Q9Y4P1
RRID: AB_2834360

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Bovine(100%), Sheep(91%), Rabbit(91%), Dog(100%)
Clonality:
Polyclonal
Specificity:
ATG4B Antibody detects endogenous levels of total ATG4B.
RRID:
AB_2834360
Cite Format: Affinity Biosciences Cat# AF0425, RRID:AB_2834360.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

APG4 autophagy 4 homolog B; APG4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); ATG4B; ATG4B_HUMAN; AUT like 1 cysteine endopeptidase; AUT-like 1 cysteine endopeptidase; AUTL1; Autophagin 1; Autophagin-1; Autophagy related cysteine endopeptidase 1; Autophagy related protein 4 homolog B; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; Cysteine protease ATG4B; hAPG4B; MGC1353;

Immunogens

Immunogen:
Uniprot:

Q9Y4P1(ATG4B_HUMAN) >>Visit HPA database.

Gene(ID):
ATG4B(23192)
Expression:
Q9Y4P1 ATG4B_HUMAN:

Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.

Description:
Autophagy is the process by which endogenous proteins and damaged organelles are destroyed intracellularly. Autophagy is postulated to be essential for cell homeostasis and cell remodeling during differentiation, metamorphosis, non-apoptotic cell death, and aging. Reduced levels of autophagy have been described in some malignant tumors, and a role for autophagy in controlling the unregulated cell growth linked to cancer has been proposed. This gene encodes a member of the autophagin protein family. The encoded protein is also designated as a member of the C-54 family of cysteine proteases. Two transcript variants that encode different isoforms were identified through sequence analysis.
Sequence:
MDAATLTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRKNFPAIGGTGPTSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFIDRKDSYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEEIRRLCRTSVPCAGATAFPADSDRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYVETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDPHTTQPAVEPTDGCFIPDESFHCQHPPCRMSIAELDPSIAVGFFCKTEDDFNDWCQQVKKLSLLGGALPMFELVELQPSHLACPDVLNLSLDSSDVERLERFFDSEDEDFEILSL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Dog
100
Sheep
91
Rabbit
91
Pig
82
Chicken
55
Zebrafish
36
Horse
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y4P1 As Substrate

Site PTM Type Enzyme
M1 Acetylation
Y8 Phosphorylation
S34 Phosphorylation
K39 Ubiquitination
S44 Phosphorylation
K102 Ubiquitination
K119 Ubiquitination
Y122 Phosphorylation
Y123 Phosphorylation
K153 Ubiquitination
S316 Phosphorylation
K337 Ubiquitination
S383 Phosphorylation
S392 Phosphorylation

Research Backgrounds

Function:

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.

Family&Domains:

Belongs to the peptidase C54 family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - other.   (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

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