DRD3 Antibody | Affinity Biosciences

WB
IF/ICC
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Western blot analysis of extracts from Colo205, using DRD3 Antibody.

Product:	DRD3 Antibody
Catalog:	DF10212
Description:	Rabbit polyclonal antibody to DRD3
Application:	WB IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	44 kDa; 44kD(Calculated).
Uniprot:	P35462
RRID:	AB_2815010

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%)

Clonality:

Polyclonal

Specificity:

DRD3 Antibody detects endogenous levels of total DRD3.

RRID:

AB_2815010
Cite Format: Affinity Biosciences Cat# DF10212, RRID:AB_2815010.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

3dopamine receptor; D; D(3) dopamine receptor; Dopamine D3 receptor; Dopamine receptor D3; DRD3; DRD3_HUMAN; ETM1; FET1;

Immunogens

Immunogen:

Uniprot:

P35462(DRD3_HUMAN) >>Visit HPA database.

Gene(ID):

DRD3(1814)

Expression:

P35462 DRD3_HUMAN:

Brain.

Sequence:

P35462 DRD3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASLSQLSSHLNYTCGAENSTGASQARPHAYYALSYCALILAIVFGNGLVCMAVLKERALQTTTNYLVVSLAVADLLVATLVMPWVVYLEVTGGVWNFSRICCDVFVTLDVMMCTASILNLCAISIDRYTAVVMPVHYQHGTGQSSCRRVALMITAVWVLAFAVSCPLLFGFNTTGDPTVCSISNPDFVIYSSVVSFYLPFGVTVLVYARIYVVLKQRRRKRILTRQNSQCNSVRPGFPQQTLSPDPAHLELKRYYSICQDTALGGPGFQERGGELKREEKTRNSLSPTIAPKLSLEVRKLSNGRLSTSLKLGPLQPRGVPLREKKATQMVAIVLGAFIVCWLPFFLTHVLNTHCQTCHVSPELYSATTWLGYVNSALNPVIYTTFNIEFRKAFLKILSC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Dog

100

Rabbit

100

Sheep

Zebrafish

Chicken

Bovine

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35462 As Substrate

P35462

Site	PTM Type	Enzyme	Source
T142	Phosphorylation		Uniprot
T225	Phosphorylation		Uniprot
S229	Phosphorylation	Q9UQM7 (CAMK2A)	Uniprot
S233	Phosphorylation		Uniprot
S257	Phosphorylation		Uniprot
S285	Phosphorylation		Uniprot
S287	Phosphorylation		Uniprot
T289	Phosphorylation		Uniprot
S302	Phosphorylation		Uniprot
S309	Phosphorylation		Uniprot

Research Backgrounds

P35462_DRD3_HUMAN

Function:

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation.

PTMs:

Phosphorylated by GRK4 (GRK4-alpha and GRK4-gamma).

Palmitoylated.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells.

Tissue Specificity:

Brain.

Subunit Structure:

Interacts with CLIC6 (By similarity). Interacts with GRK4. Interacts with PALM. Interacts with FLNA (via filamin repeat 21); increases PKA-mediated phosphorylation of FLNA.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Nervous system > Dopaminergic synapse.

References

1). Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation. Molecular Cell, 2020 (PubMed: 32035036) [IF=16.0]

Application: WB Species: Sample: BMDMs

Supplementary Figure 2.| DRD5 is required for the inhibition of TLR2-induced inflammation by DA,Related to Figure 2(C) Immunoblot analysis of the expression of DRD1-5 in Drd1-/- and Drd2-/- BMDMs or BMDMs transfected with siRNA specific against Drd3, Drd4 and Drd5 as indicated.

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DRD3 Antibody - #DF10212