Product: DRD3 Antibody
Catalog: DF10212
Description: Rabbit polyclonal antibody to DRD3
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 44 kDa; 44kD(Calculated).
Uniprot: P35462
RRID: AB_2815010

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
DRD3 Antibody detects endogenous levels of total DRD3.
RRID:
AB_2815010
Cite Format: Affinity Biosciences Cat# DF10212, RRID:AB_2815010.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3dopamine receptor; D; D(3) dopamine receptor; Dopamine D3 receptor; Dopamine receptor D3; DRD3; DRD3_HUMAN; ETM1; FET1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
MASLSQLSSHLNYTCGAENSTGASQARPHAYYALSYCALILAIVFGNGLVCMAVLKERALQTTTNYLVVSLAVADLLVATLVMPWVVYLEVTGGVWNFSRICCDVFVTLDVMMCTASILNLCAISIDRYTAVVMPVHYQHGTGQSSCRRVALMITAVWVLAFAVSCPLLFGFNTTGDPTVCSISNPDFVIYSSVVSFYLPFGVTVLVYARIYVVLKQRRRKRILTRQNSQCNSVRPGFPQQTLSPDPAHLELKRYYSICQDTALGGPGFQERGGELKREEKTRNSLSPTIAPKLSLEVRKLSNGRLSTSLKLGPLQPRGVPLREKKATQMVAIVLGAFIVCWLPFFLTHVLNTHCQTCHVSPELYSATTWLGYVNSALNPVIYTTFNIEFRKAFLKILSC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Sheep
92
Zebrafish
60
Chicken
58
Bovine
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P35462 As Substrate

Site PTM Type Enzyme
T142 Phosphorylation
T225 Phosphorylation
S229 Phosphorylation Q9UQM7 (CAMK2A)
S233 Phosphorylation
S257 Phosphorylation
S285 Phosphorylation
S287 Phosphorylation
T289 Phosphorylation
S302 Phosphorylation
S309 Phosphorylation

Research Backgrounds

Function:

Dopamine receptor whose activity is mediated by G proteins which inhibit adenylyl cyclase. Promotes cell proliferation.

PTMs:

Phosphorylated by GRK4 (GRK4-alpha and GRK4-gamma).

Palmitoylated.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Both membrane-bound and scattered in the cytoplasm during basal conditions. Receptor stimulation results in the rapid internalization and sequestration of the receptors at the perinuclear area (5 and 15 minutes), followed by the dispersal of the receptors to the membrane (30 minutes). DRD3 and GRK4 co-localize in lipid rafts of renal proximal tubule cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Brain.

Subunit Structure:

Interacts with CLIC6 (By similarity). Interacts with GRK4. Interacts with PALM. Interacts with FLNA (via filamin repeat 21); increases PKA-mediated phosphorylation of FLNA.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Nervous system > Dopaminergic synapse.

References

1). Dopamine Uses the DRD5-ARRB2-PP2A Signaling Axis to Block the TRAF6-Mediated NF-κB Pathway and Suppress Systemic Inflammation. Molecular Cell, 2020 (PubMed: 32035036) [IF=16.0]

Application: WB    Species:    Sample: BMDMs

Supplementary Figure 2.| DRD5 is required for the inhibition of TLR2-induced inflammation by DA,Related to Figure 2(C) Immunoblot analysis of the expression of DRD1-5 in Drd1-/- and Drd2-/- BMDMs or BMDMs transfected with siRNA specific against Drd3, Drd4 and Drd5 as indicated.

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For Research Use Only.
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