CCR6 Antibody - #DF10207

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Product: CCR6 Antibody
Catalog: DF10207
Description: Rabbit polyclonal antibody to CCR6
Application: WB
Reactivity: Human, Mouse
Prediction: Horse, Sheep
Mol.Wt.: 42 kDa; 42kD(Calculated).
Uniprot: P51684
RRID: AB_2840786

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Horse(100%), Sheep(100%)
Clonality:
Polyclonal
Specificity:
CCR6 Antibody detects endogenous levels of total CCR6.
RRID:
AB_2840786
Cite Format: Affinity Biosciences Cat# DF10207, RRID:AB_2840786.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BN 1; BN1; C C chemokine receptor type 6; C C CKR 6; C-C chemokine receptor type 6; C-C CKR-6; CC chemokine receptor type 6; CC CKR 6; CC R6; CC-CKR-6; CCCKR6; CCR 6; CCR-6; Ccr6; CCR6_HUMAN; CD 196; CD196; CD196 antigen; Chemokine (C C motif) receptor 6; Chemokine (C C) receptor 6; Chemokine (CC motif) receptor 6; Chemokine (CC) receptor 6; Chemokine receptor 6; Chemokine receptor like 3; Chemokine receptor-like 3; CKR 6; CKR L3; CKR-L3; CKR6; CKRL3; CMKBR 6; CMKBR6; DRY 6; DRY6; G protein coupled receptor 29; G-protein coupled receptor 29; GPR 29; GPR CY4; GPR-CY4; GPR29; GPRCY 4; GPRCY4; LARC receptor; Seven transmembrane receptor lymphocyte 22; STRL 22; STRL22;

Immunogens

Immunogen:
Uniprot:

P51684(CCR6_HUMAN) >>Visit HPA database.

Gene(ID):
CCR6(1235)
Expression:
P51684 CCR6_HUMAN:

Sperm. Mainly localized in the tail and in the postacrosomal region but is also found in the midpiece and basal region in a small percentage of sperm cells. Reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level). Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.

Sequence:
MSGESMNFSDVFDSSEDYFVSVNTSYYSVDSEMLLCSLQEVRQFSRLFVPIAYSLICVFGLLGNILVVITFAFYKKARSMTDVYLLNMAIADILFVLTLPFWAVSHATGAWVFSNATCKLLKGIYAINFNCGMLLLTCISMDRYIAIVQATKSFRLRSRTLPRSKIICLVVWGLSVIISSSTFVFNQKYNTQGSDVCEPKYQTVSEPIRWKLLMLGLELLFGFFIPLMFMIFCYTFIVKTLVQAQNSKRHKAIRVIIAVVLVFLACQIPHNMVLLVTAANLGKMNRSCQSEKLIGYTKTVTEVLAFLHCCLNPVLYAFIGQKFRNYFLKILKDLWCVRRKYKSSGFSCAGRYSENISRQTSETADNDNASSFTM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
100
Rabbit
74
Pig
67
Bovine
58
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51684 As Substrate

Site PTM Type Enzyme
Y74 Phosphorylation
Y352 Phosphorylation

Research Backgrounds

Function:

Receptor for the C-C type chemokine CCL20. Binds to CCL20 and subsequently transduces a signal by increasing the intracellular calcium ion levels. Although CCL20 is its major ligand it can also act as a receptor for non-chemokine ligands such as beta-defensins. Binds to defensin DEFB1 leading to increase in intracellular calcium ions and cAMP levels. Its binding to DEFB1 is essential for the function of DEFB1 in regulating sperm motility and bactericidal activity. Binds to defensins DEFB4 and DEFB4A/B and mediates their chemotactic effects. The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/ memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases. CCR6-mediated signals are essential for immune responses to microbes in the intestinal mucosa and in the modulation of inflammatory responses initiated by tissue insult and trauma. CCR6 is essential for the recruitment of both the proinflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. Required for the normal migration of Th17 cells in Peyers-patches and other related tissue sites of the intestine and plays a role in regulating effector T-cell balance and distribution in inflamed intestine. Plays an important role in the coordination of early thymocyte precursor migration events important for normal subsequent thymocyte precursor development, but is not required for the formation of normal thymic natural regulatory T-cells (nTregs). Required for optimal differentiation of DN2 and DN3 thymocyte precursors. Essential for B-cell localization in the subepithelial dome of Peyers-patches and for efficient B-cell isotype switching to IgA in the Peyers-patches. Essential for appropriate anatomical distribution of memory B-cells in the spleen and for the secondary recall response of memory B-cells (By similarity). Positively regulates sperm motility and chemotaxis via its binding to CCL20.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell surface.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Sperm. Mainly localized in the tail and in the postacrosomal region but is also found in the midpiece and basal region in a small percentage of sperm cells. Reduced levels found in the sperms of asthenozoospermia and leukocytospermia patients (at protein level). Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

References

1). Ginsenoside Rh1, a novel casein kinase II subunit alpha (CK2α) inhibitor, retards metastasis via disrupting HHEX/CCL20 signaling cascade involved in tumor cell extravasation across endothelial barrier. Pharmacological research, 2023 (PubMed: 37944834) [IF=9.3]

Application: WB    Species: Mouse    Sample:

Fig. 5. CCL20 orchestrated the invasiveness and metastasis of BC. (A) Representative images of CCL20 in tumor samples harvested from the patients with BC and MMTV-PyMT mice. Scale bars: 100 µm. (B) Correlation analysis of CCL20 and CCR6. (C) Quantification of CCR6 protein expression in the cells transfected with empty vector, CCL20 OE BC cells and CCL20 KO BC cells (n = 3). (D) Western blot analysis of the markers for EMT and invadopodium in the cells transfected with empty vector, CCL20 OE BC cells and CCL20 KO BC cells. (E-L) Quantification of markers for EMT and invadopodium (n = 3). Representative immunofluorescence images for the markers of invadopodium including MMP2 (M) and CTTN (N). Scale bars: 10 µm. (O) Schematic diagram for experimental metastasis model. (P) Representative high-resolution micro-CT images for lung capacity in the vector, CCL20 OE and CCL20 KO groups. (Q) The quantification of lung capacity in the empty vector, CCL20 OE and CCL20 KO groups (n = 6). (R) The quantification of loss of lung capacity in the empty vector, CCL20 OE and CCL20 KO groups (n = 6). (S) H&E staining of the livers. Scale bars: 100 µm (upper) and 200 µm (bottom). (T) Numbers of the metastatic nodules in the livers in the empty vector, CCL20 OE and CCL20 KO groups (n = 6). (U) Representative images and H&E staining of the lungs. Scale bars: 100 µm (upper panel) and 200 µm (bottom panel). (V) Numbers of the metastatic nodules in the empty vector, CCL20 OE and CCL20 KO groups (n = 6). *P 

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