CCR3 Antibody - #DF10205

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Product: CCR3 Antibody
Catalog: DF10205
Description: Rabbit polyclonal antibody to CCR3
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 41 kDa; 41kD(Calculated).
Uniprot: P51677
RRID: AB_2840784

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(83%), Bovine(92%), Horse(83%), Sheep(83%), Dog(83%)
Clonality:
Polyclonal
Specificity:
CCR3 Antibody detects endogenous levels of total CCR3.
RRID:
AB_2840784
Cite Format: Affinity Biosciences Cat# DF10205, RRID:AB_2840784.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B chemokine receptor; b-chemokine receptor; C C chemokine receptor type 3; C C CKR 3; C-C chemokine receptor type 3; C-C CKR-3; CC chemokine receptor 3; CC chemokine receptor type 3; CC CKR 3; CC R3; CC-CKR-3; CCCKR3; CCR 3; CCR-3; CCR3; CCR3_HUMAN; CD 193; CD193; CD193 antigen; Chemokine (C C motif) receptor 3; chemokine (C-C motif) receptor 3; Chemokine (CC) receptor 1 like 2; Chemokine CC motif receptor 3; Chemokine receptor 3; CK R3; CKR 3; CKR3; CMKB R3; CMKBR 3; CMKBR1L2; CMKBR3; Eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; Eotaxin receptor; Macrophage inflammatory protein 1 alpha receptor like 2; MGC102841; MIP1 Alpha RL2;

Immunogens

Immunogen:
Uniprot:

P51677(CCR3_HUMAN) >>Visit HPA database.

Gene(ID):
CCR3(1232)
Expression:
P51677 CCR3_HUMAN:

In eosinophils as well as trace amounts in neutrophils and monocytes.

Sequence:
MTTSLDTVETFGTTSYYDDVGLLCEKADTRALMAQFVPPLYSLVFTVGLLGNVVVVMILIKYRRLRIMTNIYLLNLAISDLLFLVTLPFWIHYVRGHNWVFGHGMCKLLSGFYHTGLYSEIFFIILLTIDRYLAIVHAVFALRARTVTFGVITSIVTWGLAVLAALPEFIFYETEELFEETLCSALYPEDTVYSWRHFHTLRMTIFCLVLPLLVMAICYTGIIKTLLRCPSKKKYKAIRLIFVIMAVFFIFWTPYNVAILLSSYQSILFGNDCERSKHLDLVMLVTEVIAYSHCCMNPVIYAFVGERFRKYLRHFFHRHLLMHLGRYIPFLPSEKLERTSSVSPSTAEPELSIVF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
92
Pig
83
Horse
83
Sheep
83
Dog
83
Rabbit
67
Xenopus
50
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Receptor for C-C type chemokine. Binds and responds to a variety of chemokines, including CCL11, CCL26, CCL7, CCL13, RANTES(CCL5) and CCL15. Subsequently transduces a signal by increasing the intracellular calcium ions level. In addition acts as a possible functional receptor for NARS1.

(Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

In eosinophils as well as trace amounts in neutrophils and monocytes.

Subunit Structure:

(Microbial infection) Interacts with HIV-1 protein Tat.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Human Diseases > Cancers: Overview > Viral carcinogenesis.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

References

1). SIRT1 regulates O-GlcNAcylation of tau through OGT. Aging-US, 2020 (PubMed: 32310828) [IF=5.2]

Application: WB    Species: human    Sample: HEK-293T cells

Figure 1. |SIRT1 decreases protein O-GlcNAcylation of tau in cells.(B) shSIRT1 plasmids or its control plasmids were transfected into HEK-293T cells. Recombinant human tau441 was immunoaffinity-purified with anti-GFP antibody from cells. Quantitative analysis of relative O-GlcNAcylation levels of tau after being normalized with total tau is shown as mean ± S.D. (n=3), ***, p < 0.001.
2). High CCL7 expression is associated with migration, invasion and bone metastasis of non-small cell lung cancer cells. American Journal of Translational Research, 2019 (PubMed: 30788000) [IF=2.2]

Application: IHC    Species: human    Sample: primary NSCLC and bone metastasis tissues

Figure 2. |Expression of possible target chemokine receptors of CCL7 in primary NSCLC and bone metastasis tissues. A-C. IHC detection of CCR1, CCR2 and CCR3 expression in primary NSCLC and bone metastasis tissues. CCR1, CCR2 and CCR3 were mainly expressed on the cell membrane and in cytoplasm (arrow). D. qRT-PCR measurement of mRNA levels of CCR1, CCR2, CCR3 in primary NSCLC and bone metastasis tissues. *: P < 0.05.

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