CALCRL Antibody | Affinity Biosciences

Cites

1/2

Product:	CALCRL Antibody
Catalog:	DF10203
Description:	Rabbit polyclonal antibody to CALCRL
Application:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	53 kDa; 53kD(Calculated).
Uniprot:	Q16602
RRID:	AB_2840782

View similar products>>

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors

Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(86%)

Clonality:

Polyclonal

Specificity:

CALCRL Antibody detects endogenous levels of total CALCRL.

RRID:

AB_2840782
Cite Format: Affinity Biosciences Cat# DF10203, RRID:AB_2840782.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Calcitonin gene related peptide type 1 receptor; Calcitonin receptor like; Calcitonin receptor like receptor; CALCRL; CGRP type 1 receptor; CGRPR; CRLR;

Immunogens

Immunogen:

Uniprot:

Q16602(CALRL_HUMAN) >>Visit HPA database.

Gene(ID):

CALCRL(10203)

Expression:

Q16602 CALRL_HUMAN:

Predominantly expressed in the lung and heart.

Sequence:

Q16602 CALRL_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEKKCTLNFLVLLPFFMILVTAELEESPEDSIQLGVTRNKIMTAQYECYQKIMQDPIQQAEGVYCNRTWDGWLCWNDVAAGTESMQLCPDYFQDFDPSEKVTKICDQDGNWFRHPASNRTWTNYTQCNVNTHEKVKTALNLFYLTIIGHGLSIASLLISLGIFFYFKSLSCQRITLHKNLFFSFVCNSVVTIIHLTAVANNQALVATNPVSCKVSQFIHLYLMGCNYFWMLCEGIYLHTLIVVAVFAEKQHLMWYYFLGWGFPLIPACIHAIARSLYYNDNCWISSDTHLLYIIHGPICAALLVNLFFLLNIVRVLITKLKVTHQAESNLYMKAVRATLILVPLLGIEFVLIPWRPEGKIAEEVYDYIMHILMHFQGLLVSTIFCFFNGEVQAILRRNWNQYKIQFGNSFSNSEALRSASYTVSTISDGPGYSHDCPSEHLNGKSIHDIENVLLKPENLYN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Dog

100

Pig

Horse

Bovine

Sheep

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16602 As Substrate

Q16602

Site	PTM Type	Source
S285	Phosphorylation	Uniprot
K403	Ubiquitination	Uniprot
S409	Phosphorylation	Uniprot
S445	Phosphorylation	Uniprot
Y460	Phosphorylation	Uniprot

Research Backgrounds

Q16602_CALRL_HUMAN

Function:

Receptor for calcitonin-gene-related peptide (CGRP) together with RAMP1 and receptor for adrenomedullin together with RAMP3 (By similarity). Receptor for adrenomedullin together with RAMP2. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Predominantly expressed in the lung and heart.

Subunit Structure:

Heterodimer of CALCRL and RAMP3 (By similarity). Heterodimer of CALCRL and RAMP1 or CALCRL and RAMP2.

Family&Domains:

Belongs to the G-protein coupled receptor 2 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Circulatory system > Vascular smooth muscle contraction. (View pathway)

References

1). Intermedin in Paraventricular Nucleus Attenuates Ang II-Induced Sympathoexcitation through the Inhibition of NADPH Oxidase-Dependent ROS Generation in Obese Rats with Hypertension. International Journal of Molecular Sciences, 2019 (PubMed: 31466304) [IF=5.6]

Application: WB Species: rat Sample: paraventricular nucleus

Figure 1. |Plasma norepinephrine (NE) level (A. n = 6 or 7), systolic blood pressure (B. n = 6–7); and relative values of intermedin (IMD), calcitonin receptor–like receptor (CRLR) and receptor activity–modifying protein (RAMP) 1, RAMP2, and RAMP3 protein expression in the paraventricular nucleus (PVN) in control and obesity-related hypertensive (OH) rats (C–H. n = 3–4). Values are mean± SEM. * p < 0.05 versus control.

2). Exogenous CGRP Regulates Apoptosis and Autophagy to Alleviate Traumatic Brain Injury Through Akt/mTOR Signalling Pathway. NEUROCHEMICAL RESEARCH, 2020 (PubMed: 33063244) [IF=4.4]

Application: WB Species: mouse Sample: brain cortical

Fig. 1 CGRP alleviated the brain lesion induced by TBI. a Relative protein expression of CGRP in the brain cortical tissues with or without CGRP administration after TBI over time was detected by western blot (loading volume of CGRP: 15 μg).

3). Adrenomedullin ameliorates palmitic acid-induced insulin resistance through PI3K/Akt pathway in adipocytes. ACTA DIABETOLOGICA, 2022 (PubMed: 34978596) [IF=3.8]

4). Correlation of vascular change with TRPV1, TRPV4, and TRPA1 in a rat model of inferior gluteal artery perforator flap. WOUND REPAIR AND REGENERATION, 2022 (PubMed: 35384152) [IF=2.9]

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

CALCRL Antibody - #DF10203