CALCR Antibody | Affinity Biosciences

IHC
IF/ICC
Cites

1/5

DF10202 at 1/100 staining Mouse lung tissue by IHC-P.

DF10202 at 1/100 staining Mouse kidney tissue by IHC-P.

DF10202 staining Hela cells by IF/ICC.

Figure 3.

Product:	CALCR Antibody
Catalog:	DF10202
Description:	Rabbit polyclonal antibody to CALCR
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	59 kDa; 55kD(Calculated).
Uniprot:	P30988
RRID:	AB_2840781

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

CALCR Antibody detects endogenous levels of total CALCR.

RRID:

AB_2840781
Cite Format: Affinity Biosciences Cat# DF10202, RRID:AB_2840781.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Calcitonin receptor; Calcitonin receptor precursor; CALCR; CALCR_HUMAN; CT R; CT-R; CTR; CTR1;

Immunogens

Immunogen:

Uniprot:

P30988(CALCR_HUMAN) >>Visit HPA database.

Gene(ID):

CALCR(799)

Sequence:

P30988 CALCR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MRFTFTSRCLALFLLLNHPTPILPAFSNQTYPTIEPKPFLYVVGRKKMMDAQYKCYDRMQQLPAYQGEGPYCNRTWDGWLCWDDTPAGVLSYQFCPDYFPDFDPSEKVTKYCDEKGVWFKHPENNRTWSNYTMCNAFTPEKLKNAYVLYYLAIVGHSLSIFTLVISLGIFVFFRSLGCQRVTLHKNMFLTYILNSMIIIIHLVEVVPNGELVRRDPVSCKILHFFHQYMMACNYFWMLCEGIYLHTLIVVAVFTEKQRLRWYYLLGWGFPLVPTTIHAITRAVYFNDNCWLSVETHLLYIIHGPVMAALVVNFFFLLNIVRVLVTKMRETHEAESHMYLKAVKATMILVPLLGIQFVVFPWRPSNKMLGKIYDYVMHSLIHFQGFFVATIYCFCNNEVQTTVKRQWAQFKIQWNQRWGRRPSNRSARAAAAAAEAGDIPIYICHQEPRNEPANNQGEESAEIIPLNIIEQESSA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30988 As Substrate

P30988

Site	PTM Type	Source
Y59	Phosphorylation	Uniprot
Y71	Phosphorylation	Uniprot
Y74	Phosphorylation	Uniprot
Y149	Phosphorylation	Uniprot
T150	Phosphorylation	Uniprot
T156	Phosphorylation	Uniprot
T196	Phosphorylation	Uniprot
S252	Phosphorylation	Uniprot

Research Backgrounds

P30988_CALCR_HUMAN

Function:

This is a receptor for calcitonin. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The calcitonin receptor is thought to couple to the heterotrimeric guanosine triphosphate-binding protein that is sensitive to cholera toxin.

Receptor for calcitonin but is unable to couple to G proteins and activate adenylyl cyclase. Does not undergo receptor internalization following ligand binding.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Subunit Structure:

Interacts with GPRASP2.

Family&Domains:

Belongs to the G-protein coupled receptor 2 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Development > Osteoclast differentiation. (View pathway)

References

1). Design of a self-driven probiotic-CRISPR/Cas9 nanosystem for sono-immunometabolic cancer therapy. Nature Communications, 2022 (PubMed: 36550159) [IF=16.6]

2). Multistage Cooperative Nanodrug Combined with PD‐L1 for Enhancing Antitumor Chemoimmunotherapy. Advanced Healthcare Materials, 2021 (PubMed: 34382363) [IF=10.0]

3). Simvastatin improves oral implant osseointegration via enhanced autophagy and osteogenesis of BMSCs and inhibited osteoclast activity. Journal of Tissue Engineering and Regenerative Medicine, 2018 (PubMed: 29498229) [IF=3.3]

Application: WB Species: rat Sample: Osteoclast

Figure 3.| Simvastatin decreases osteoclast numbers and function.(E) Osteoclast differentiation markers (CTSK, CALCR, ITGβ3) were analyzed by Western blot. Cells were treated with PBS (control) or simvastatin (Sim). *p< 0.05, **p< 0.01. Scale bars: 100 μm (A, B). Data are presented as the mean ± standard deviation, n=3.

4). Deletion of osteopontin in non-small cell lung cancer cells affects bone metabolism by regulating miR-34c/Notch1 axis: a clue to bone metastasis. European Journal of Histochemistry, 2023 (PubMed: 37491944) [IF=2.0]

Application: WB Species: Human Sample: SaOS-2 cells

Figure 3.Deletion of osteopontin (OPN) inhibited the differentiation of osteoclasts. A) RANK-positive rate of RAW264.7 cells was detected by flow cytometry. B) The protein expressions of OCs markers Cathepsin K and CTR were detected by Western blot. C) The area of F-actin ring was evaluated by immunofluorescence staining.

5). Self-Assembled Immunostimulatory Nanodrug Combined with PD-L1 for Boosting Anti-Tumor Chemoimmunotherapy. , 2022

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CALCR Antibody - #DF10202