CALCR Antibody - #DF10202

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Product: CALCR Antibody
Catalog: DF10202
Description: Rabbit polyclonal antibody to CALCR
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 59 kDa; 55kD(Calculated).
Uniprot: P30988
RRID: AB_2840781

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(86%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
CALCR Antibody detects endogenous levels of total CALCR.
RRID:
AB_2840781
Cite Format: Affinity Biosciences Cat# DF10202, RRID:AB_2840781.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Calcitonin receptor; Calcitonin receptor precursor; CALCR; CALCR_HUMAN; CT R; CT-R; CTR; CTR1;

Immunogens

Immunogen:
Uniprot:

P30988(CALCR_HUMAN) >>Visit HPA database.

Gene(ID):
CALCR(799)
Sequence:
MRFTFTSRCLALFLLLNHPTPILPAFSNQTYPTIEPKPFLYVVGRKKMMDAQYKCYDRMQQLPAYQGEGPYCNRTWDGWLCWDDTPAGVLSYQFCPDYFPDFDPSEKVTKYCDEKGVWFKHPENNRTWSNYTMCNAFTPEKLKNAYVLYYLAIVGHSLSIFTLVISLGIFVFFRSLGCQRVTLHKNMFLTYILNSMIIIIHLVEVVPNGELVRRDPVSCKILHFFHQYMMACNYFWMLCEGIYLHTLIVVAVFTEKQRLRWYYLLGWGFPLVPTTIHAITRAVYFNDNCWLSVETHLLYIIHGPVMAALVVNFFFLLNIVRVLVTKMRETHEAESHMYLKAVKATMILVPLLGIQFVVFPWRPSNKMLGKIYDYVMHSLIHFQGFFVATIYCFCNNEVQTTVKRQWAQFKIQWNQRWGRRPSNRSARAAAAAAEAGDIPIYICHQEPRNEPANNQGEESAEIIPLNIIEQESSA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
86
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P30988 As Substrate

Site PTM Type Enzyme
Y59 Phosphorylation
Y71 Phosphorylation
Y74 Phosphorylation
Y149 Phosphorylation
T150 Phosphorylation
T156 Phosphorylation
T196 Phosphorylation
S252 Phosphorylation

Research Backgrounds

Function:

This is a receptor for calcitonin. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. The calcitonin receptor is thought to couple to the heterotrimeric guanosine triphosphate-binding protein that is sensitive to cholera toxin.

Receptor for calcitonin but is unable to couple to G proteins and activate adenylyl cyclase. Does not undergo receptor internalization following ligand binding.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with GPRASP2.

Family&Domains:

Belongs to the G-protein coupled receptor 2 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

References

1). Design of a self-driven probiotic-CRISPR/Cas9 nanosystem for sono-immunometabolic cancer therapy. Nature Communications (PubMed: 36550159) [IF=16.6]
2). Multistage Cooperative Nanodrug Combined with PD‐L1 for Enhancing Antitumor Chemoimmunotherapy. Advanced Healthcare Materials (PubMed: 34382363) [IF=10.0]
3). Simvastatin improves oral implant osseointegration via enhanced autophagy and osteogenesis of BMSCs and inhibited osteoclast activity. Journal of Tissue Engineering and Regenerative Medicine (PubMed: 29498229) [IF=3.3]

Application: WB    Species: rat    Sample: Osteoclast

Figure 3.| Simvastatin decreases osteoclast numbers and function.(E) Osteoclast differentiation markers (CTSK, CALCR, ITGβ3) were analyzed by Western blot. Cells were treated with PBS (control) or simvastatin (Sim). *p< 0.05, **p< 0.01. Scale bars: 100 μm (A, B). Data are presented as the mean ± standard deviation, n=3.
4). Deletion of osteopontin in non-small cell lung cancer cells affects bone metabolism by regulating miR-34c/Notch1 axis: a clue to bone metastasis. European Journal of Histochemistry (PubMed: 37491944) [IF=2.0]

Application: WB    Species: Human    Sample: SaOS-2 cells

Figure 3.Deletion of osteopontin (OPN) inhibited the differentiation of osteoclasts. A) RANK-positive rate of RAW264.7 cells was detected by flow cytometry. B) The protein expressions of OCs markers Cathepsin K and CTR were detected by Western blot. C) The area of F-actin ring was evaluated by immunofluorescence staining.
5). Self-Assembled Immunostimulatory Nanodrug Combined with PD-L1 for Boosting Anti-Tumor Chemoimmunotherapy.

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