C3AR1 Antibody | Affinity Biosciences

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Product:	C3AR1 Antibody
Catalog:	DF10198
Description:	Rabbit polyclonal antibody to C3AR1
Application:	WB
Reactivity:	Human
Prediction:	Rabbit
Mol.Wt.:	54 kDa; 54kD(Calculated).
Uniprot:	Q16581
RRID:	AB_2840777

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Secondary Antibodies
Blocking Peptides

Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Prediction:

Rabbit(83%)

Clonality:

Polyclonal

Specificity:

C3AR1 Antibody detects endogenous levels of total C3AR1.

RRID:

AB_2840777
Cite Format: Affinity Biosciences Cat# DF10198, RRID:AB_2840777.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AZ 3B; AZ3B; C3a anaphylatoxin chemotactic receptor; C3A R; C3A R1; C3a-R; C3AR; C3AR_HUMAN; C3AR1; C3R1; Complement component 3 receptor 1; Complement component 3a receptor 1; HNFAG09;

Immunogens

Immunogen:

Uniprot:

Q16581(C3AR_HUMAN) >>Visit HPA database.

Gene(ID):

C3AR1(719)

Expression:

Q16581 C3AR_HUMAN:

Widely expressed in several differentiated hematopoietic cell lines, in the lung, spleen, ovary, placenta, small intestine, throughout the brain, heart, and endothelial cells. Mostly expressed in lymphoid tissues.

Sequence:

Q16581 C3AR_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASFSAETNSTDLLSQPWNEPPVILSMVILSLTFLLGLPGNGLVLWVAGLKMQRTVNTIWFLHLTLADLLCCLSLPFSLAHLALQGQWPYGRFLCKLIPSIIVLNMFASVFLLTAISLDRCLVVFKPIWCQNHRNVGMACSICGCIWVVAFVMCIPVFVYREIFTTDNHNRCGYKFGLSSSLDYPDFYGDPLENRSLENIVQPPGEMNDRLDPSSFQTNDHPWTVPTVFQPQTFQRPSADSLPRGSARLTSQNLYSNVFKPADVVSPKIPSGFPIEDHETSPLDNSDAFLSTHLKLFPSASSNSFYESELPQGFQDYYNLGQFTDDDQVPTPLVAITITRLVVGFLLPSVIMIACYSFIVFRMQRGRFAKSQSKTFRVAVVVVAVFLVCWTPYHIFGVLSLLTDPETPLGKTLMSWDHVCIALASANSCFNPFLYALLGKDFRKKARQSIQGILEAAFSEELTRSTHCPSNNVISERNSTTV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Bovine

Horse

Sheep

Dog

Chicken

Pig

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16581 As Substrate

Q16581

Site	PTM Type	Source
S266	O-Glycosylation	Uniprot
S266	Phosphorylation	Uniprot
S349	Phosphorylation	Uniprot
S459	Phosphorylation	Uniprot
T463	Phosphorylation	Uniprot
S465	Phosphorylation	Uniprot
T466	Phosphorylation	Uniprot
S470	Phosphorylation	Uniprot
S475	Phosphorylation	Uniprot
S479	Phosphorylation	Uniprot
T480	Phosphorylation	Uniprot
T481	Phosphorylation	Uniprot

Research Backgrounds

Q16581_C3AR_HUMAN

Function:

Receptor for the chemotactic and inflammatory peptide anaphylatoxin C3a. This receptor stimulates chemotaxis, granule enzyme release and superoxide anion production.

PTMs:

Among the sulfation sites Tyr-174 is essential for binding of C3a anaphylatoxin.

O-glycosylated.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

Subunit Structure:

Interacts with VGF-derived peptide TLQP-21 (By similarity).

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.

· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)

References

1). Aristolochic acid I promotes the invasion and migration of hepatocellular carcinoma cells by activating the C3a/C3aR complement system. TOXICOLOGY LETTERS, 2020 (PubMed: 32898628) [IF=3.5]

Application: WB Species: mouse Sample: Hepa1-6 cells

Fig 4.| The eff ects of AAI exposure on C3a secretion and C3aR mRNA and protein expression in Hepa1-6 cells. Hepa1-6 cells were exposed to diff erent concentrations of AAI (0, 2.5, 5, or 10 μM) for 24 h. (A) Enzyme-linked immunosorbent assay was performed to measure AAI-induced excess C3a secretion (B) Western blot assays were performed to measure the expression of the C3aR protein.

Application: IF/ICC Species: mouse Sample: Hepa1-6 cells

Fig 4.| The eff ects of AAI exposure on C3a secretion and C3aR mRNA and protein expression in Hepa1-6 cells. Hepa1-6 cells were exposed to diff erent concentrations of AAI (0, 2.5, 5, or 10 μM) for 24 h. (D) Immunofluorescence images stained for C3aR (green) and DAPI (blue) Scale bar, 50 μm. Each bar represents the mean ±SD based on three replicates. P < 0.05 was considered to be statistically significant. ** compared with vehicle control (0 μM), P <0.01; ***compared with vehicle control (0 μM), P < 0.001; ****compared with vehicle control(0 μM), P < 0.0001

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C3AR1 Antibody - #DF10198