HTR3C Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis of extracts from Rat kidney, using HTR3C Antibody.

Product:	HTR3C Antibody
Catalog:	DF10196
Description:	Rabbit polyclonal antibody to HTR3C
Application:	WB IF/ICC
Reactivity:	Human, Rat
Mol.Wt.:	50 kDa; 50kD(Calculated).
Uniprot:	Q8WXA8
RRID:	AB_2840775

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Clonality:

Polyclonal

Specificity:

HTR3C Antibody detects endogenous levels of total HTR3C.

RRID:

AB_2840775
Cite Format: Affinity Biosciences Cat# DF10196, RRID:AB_2840775.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

5 HT3 C; 5 HT3C; 5 hydroxytryptamine (serotonin) receptor 3, family member C; 5 hydroxytryptamine (serotonin) receptor 3C ionotropic; 5 hydroxytryptamine receptor 3 subunit C; 5 hydroxytryptamine receptor 3C; Serotonin receptor 3C;

Immunogens

Immunogen:

Uniprot:

Q8WXA8(5HT3C_HUMAN) >>Visit HPA database.

Gene(ID):

HTR3C(170572)

Expression:

Q8WXA8 5HT3C_HUMAN:

Expressed in many tissues including adult brain, colon, intestine, lung, muscle and stomach as well as fetal colon and kidney.

Sequence:

Q8WXA8 5HT3C_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEGGWPARQSALLCLTVSLLLQGRGDAFTINCSGFDQHGVDPAVFQAVFDRKAFRPFTNYSIPTRVNISFTLSAILGVDAQLQLLTSFLWMDLVWDNPFINWNPKECVGINKLTVLAENLWLPDIFIVESMDVDQTPSGLTAYISSEGRIKYDKPMRVTSICNLDIFYFPFDQQNCTFTFSSFLYTVDSMLLGMDKEVWEITDTSRKVIQTQGEWELLGINKATPKMSMGNNLYDQIMFYVAIRRRPSLYIINLLVPSSFLVAIDALSFYLPAESENRAPFKITLLLGYNVFLLMMNDLLPASGTPLISVYFALCLSLMVVSLLETVFITYLLHVATTQPPPMPRWLHSLLLHCTSPGRCCPTAPQKGNKGLGLTLTHLPGPKEPGELAGKKLGPRETEPDGGSGWTKTQLMELWVQFSHAMDTLLFRLYLLFMASSILTVIVLWNT

PTMs - Q8WXA8 As Substrate

Q8WXA8

Site	PTM Type	Source
Y234	Phosphorylation	Uniprot
T398	Phosphorylation	Uniprot
S404	Phosphorylation	Uniprot
T407	Phosphorylation	Uniprot

Research Backgrounds

Q8WXA8_5HT3C_HUMAN

Function:

This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Presumably retained within the endoplasmic reticulum unless complexed with HTR3A.

Tissue Specificity:

Expressed in many tissues including adult brain, colon, intestine, lung, muscle and stomach as well as fetal colon and kidney.

Subunit Structure:

Forms a pentaheteromeric complex with HTR3A. Not functional as a homomeric complex.

Family&Domains:

Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3C sub-subfamily.

Research Fields

· Organismal Systems > Nervous system > Serotonergic synapse.

· Organismal Systems > Sensory system > Taste transduction.

Restrictive clause

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For Research Use Only.
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HTR3C Antibody - #DF10196