LTBP1 Antibody | Affinity Biosciences

WB
Cites

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Western blot analysis of LTBP1 using HeLa whole cell lysates.

Figure 6 F-actin and LTBP1 under static and flow conditions in the 2D model.

Product:	LTBP1 Antibody
Catalog:	DF10191
Description:	Rabbit polyclonal antibody to LTBP1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	187kDa; 187kD(Calculated).
Uniprot:	Q14766
RRID:	AB_2840770

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(89%), Bovine(89%), Horse(100%), Sheep(89%), Rabbit(100%), Dog(100%), Chicken(100%)

Clonality:

Polyclonal

Specificity:

LTBP1 Antibody detects endogenous levels of total LTBP1.

RRID:

AB_2840770
Cite Format: Affinity Biosciences Cat# DF10191, RRID:AB_2840770.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

9430031G15Rik; 9830146M04; Latent transforming growth factor beta binding protein 1; Latent-transforming growth factor beta-binding protein 1; Latent-transforming growth factor beta-binding protein, isoform 1L; Latent-transforming growth factor beta-binding protein, isoform 1S; LBT1S; LTBP-1; Ltbp1; LTBP1_HUMAN; MGC163161; TGF-beta1-BP-1; Tgfb; Transforming growth factor beta-1-binding protein 1; Transforming growth factor beta-1-masking protein large subunit;

Immunogens

Immunogen:

Uniprot:

Q14766(LTBP1_HUMAN) >>Visit HPA database.

Gene(ID):

LTBP1(4052)

Expression:

Q14766 LTBP1_HUMAN:

Expressed in the aorta (at protein level) (PubMed:17293099). Isoform Long: Expressed in fibroblasts (PubMed:17293099).

Sequence:

Q14766 LTBP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGAWLRWGLLLWAGLLASSAHGRLRRITYVVHPGPGLAAGALPLSGPPRSRTFNVALNARYSRSSAAAGAPSRASPGVPSERTRRTSKPGGAALQGLRPPPPPPPEPARPAVPGGQLHPNPGGHPAAAPFTKQGRQVVRSKVPQETQSGGGSRLQVHQKQQLQGVNVCGGRCCHGWSKAPGSQRCTKPSCVPPCQNGGMCLRPQLCVCKPGTKGKACETIAAQDTSSPVFGGQSPGAASSWGPPEQAAKHTSSKKADTLPRVSPVAQMTLTLKPKPSVGLPQQIHSQVTPLSSQSVVIHHGQTQEYVLKPKYFPAQKGISGEQSTEGSFPLRYVQDQVAAPFQLSNHTGRIKVVFTPSICKVTCTKGSCQNSCEKGNTTTLISENGHAADTLTATNFRVVICHLPCMNGGQCSSRDKCQCPPNFTGKLCQIPVHGASVPKLYQHSQQPGKALGTHVIHSTHTLPLTVTSQQGVKVKFPPNIVNIHVKHPPEASVQIHQVSRIDGPTGQKTKEAQPGQSQVSYQGLPVQKTQTIHSTYSHQQVIPHVYPVAAKTQLGRCFQETIGSQCGKALPGLSKQEDCCGTVGTSWGFNKCQKCPKKPSYHGYNQMMECLPGYKRVNNTFCQDINECQLQGVCPNGECLNTMGSYRCTCKIGFGPDPTFSSCVPDPPVISEEKGPCYRLVSSGRQCMHPLSVHLTKQLCCCSVGKAWGPHCEKCPLPGTAAFKEICPGGMGYTVSGVHRRRPIHHHVGKGPVFVKPKNTQPVAKSTHPPPLPAKEEPVEALTFSREHGPGVAEPEVATAPPEKEIPSLDQEKTKLEPGQPQLSPGISTIHLHPQFPVVIEKTSPPVPVEVAPEASTSSASQVIAPTQVTEINECTVNPDICGAGHCINLPVRYTCICYEGYRFSEQQRKCVDIDECTQVQHLCSQGRCENTEGSFLCICPAGFMASEEGTNCIDVDECLRPDVCGEGHCVNTVGAFRCEYCDSGYRMTQRGRCEDIDECLNPSTCPDEQCVNSPGSYQCVPCTEGFRGWNGQCLDVDECLEPNVCANGDCSNLEGSYMCSCHKGYTRTPDHKHCRDIDECQQGNLCVNGQCKNTEGSFRCTCGQGYQLSAAKDQCEDIDECQHRHLCAHGQCRNTEGSFQCVCDQGYRASGLGDHCEDINECLEDKSVCQRGDCINTAGSYDCTCPDGFQLDDNKTCQDINECEHPGLCGPQGECLNTEGSFHCVCQQGFSISADGRTCEDIDECVNNTVCDSHGFCDNTAGSFRCLCYQGFQAPQDGQGCVDVNECELLSGVCGEAFCENVEGSFLCVCADENQEYSPMTGQCRSRTSTDLDVDVDQPKEEKKECYYNLNDASLCDNVLAPNVTKQECCCTSGVGWGDNCEIFPCPVLGTAEFTEMCPKGKGFVPAGESSSEAGGENYKDADECLLFGQEICKNGFCLNTRPGYECYCKQGTYYDPVKLQCFDMDECQDPSSCIDGQCVNTEGSYNCFCTHPMVLDASEKRCIRPAESNEQIEETDVYQDLCWEHLSDEYVCSRPLVGKQTTYTECCCLYGEAWGMQCALCPLKDSDDYAQLCNIPVTGRRQPYGRDALVDFSEQYTPEADPYFIQDRFLNSFEELQAEECGILNGCENGRCVRVQEGYTCDCFDGYHLDTAKMTCVDVNECDELNNRMSLCKNAKCINTDGSYKCLCLPGYVPSDKPNYCTPLNTALNLEKDSDLE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Dog

100

Chicken

100

Rabbit

100

Pig

Bovine

Sheep

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14766 As Substrate

Q14766

Site	PTM Type	Source
S66	Phosphorylation	Uniprot
S296	O-Glycosylation	Uniprot
T304	O-Glycosylation	Uniprot
K318	Ubiquitination	Uniprot
S321	Phosphorylation	Uniprot
S325	Phosphorylation	Uniprot
T326	O-Glycosylation	Uniprot
T357	O-Glycosylation	Uniprot
S438	O-Glycosylation	Uniprot
S460	O-Glycosylation	Uniprot
T461	O-Glycosylation	Uniprot
T463	O-Glycosylation	Uniprot
T467	O-Glycosylation	Uniprot
T469	O-Glycosylation	Uniprot
S470	O-Glycosylation	Uniprot
S501	O-Glycosylation	Uniprot
Y523	Phosphorylation	Uniprot
T531	Phosphorylation	Uniprot
T533	O-Glycosylation	Uniprot
S536	O-Glycosylation	Uniprot
T537	O-Glycosylation	Uniprot
S539	O-Glycosylation	Uniprot
S738	Phosphorylation	Uniprot
S768	O-Glycosylation	Uniprot
T769	O-Glycosylation	Uniprot
T785	O-Glycosylation	Uniprot
T801	O-Glycosylation	Uniprot
S810	O-Glycosylation	Uniprot
Y896	Phosphorylation	Uniprot
T897	Phosphorylation	Uniprot
Y901	Phosphorylation	Uniprot
Y904	Phosphorylation	Uniprot
Y988	Phosphorylation	Uniprot
T991	Phosphorylation	Uniprot
T1333	O-Glycosylation	Uniprot
S1413	Phosphorylation	Uniprot
S1414	Phosphorylation	Uniprot
S1415	Phosphorylation	Uniprot
Y1422	Phosphorylation	Uniprot
S1597	Phosphorylation	Uniprot
Y1600	Phosphorylation	Uniprot
T1601	Phosphorylation	Uniprot
S1616	Phosphorylation	Uniprot
Y1688	Phosphorylation	Uniprot

Research Backgrounds

Q14766_LTBP1_HUMAN

Function:

Key regulator of transforming growth factor beta (TGFB1, TGFB2 and TGFB3) that controls TGF-beta activation by maintaining it in a latent state during storage in extracellular space. Associates specifically via disulfide bonds with the Latency-associated peptide (LAP), which is the regulatory chain of TGF-beta, and regulates integrin-dependent activation of TGF-beta. Outcompeted by LRRC32/GARP for binding to LAP regulatory chain of TGF-beta.

PTMs:

Contains hydroxylated asparagine residues.

Isoform Short N-terminus is blocked.

Two intrachain disulfide bonds from the TB3 domain are rearranged upon TGFB1 binding, and form interchain bonds with TGFB1 propeptide, anchoring it to the extracellular matrix.

Subcellular Location:

Secreted. Secreted>Extracellular space>Extracellular matrix.

Tissue Specificity:

Expressed in the aorta (at protein level). Isoform Long: Expressed in fibroblasts.

Subunit Structure:

Interacts with TGFB1; associates via disulfide bonds with the Latency-associated peptide chain (LAP) regulatory chain of TGFB1, leading to regulate activation of TGF-beta-1. LTBP1 does not bind directly to TGF-beta-1, the active chain of TGFB1. Interacts (via C-terminal domain) with FBN1 (via N-terminal domain). Interacts with FBN2. Interacts with ADAMTSL2.

Family&Domains:

The 8-Cys3 region in the third TB domain mediates the interchain disulfide bond interaction with the Latency-associated peptide chain (LAP) regulatory chain of TGFB1.

Belongs to the LTBP family.

Research Fields

· Environmental Information Processing > Signal transduction > TGF-beta signaling pathway. (View pathway)

References

1). Slow Interstitial Fluid Flow Activates TGF-β Signaling and Drives Fibrotic Responses in Human Tenon Fibroblasts. Cells, 2023 (PubMed: 37681937) [IF=6.0]

Application: IHC Species: Human Sample:

Figure 6 F-actin and LTBP1 under static and flow conditions in the 2D model. Effect of continuous flow (150 µL/h, 72 h) on LTBP1 in the 2D model. Image acquisition and representation settings are identical for all conditions. The figure is representative of three independent experiments.

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LTBP1 Antibody - #DF10191