Product: ENOSF1 Antibody
Catalog: DF10190
Description: Rabbit polyclonal antibody to ENOSF1
Application: WB
Reactivity: Human
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 50 kDa; 50kD(Calculated).
Uniprot: Q7L5Y1
RRID: AB_2840769

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(83%), Dog(92%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
ENOSF1 Antibody detects endogenous levels of total ENOSF1.
RRID:
AB_2840769
Cite Format: Affinity Biosciences Cat# DF10190, RRID:AB_2840769.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Antisense RNA to thymidylate synthase; ENOF1_HUMAN; Enolase superfamily member 1; ENOSF 1; enosf1; HSRTSBETA; Mitochondrial enolase superfamily member 1; RTS alpha; RTS; RTS beta; RTS beta protein; TYMSAS;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MVRGRISRLSVRDVRFPTSLGGHGADAMHTDPDYSAAYVVIETDAEDGIKGCGITFTLGKGTEVVVCAVNALAHHVLNKDLKDIVGDFRGFYRQLTSDGQLRWIGPEKGVVHLATAAVLNAVWDLWAKQEGKPVWKLLVDMDPRMLVSCIDFRYITDVLTEEDALEILQKGQIGKKEREKQMLAQGYPAYTTSCAWLGYSDDTLKQLCAQALKDGWTRFKVKVGADLQDDMRRCQIIRDMIGPEKTLMMDANQRWDVPEAVEWMSKLAKFKPLWIEEPTSPDDILGHATISKALVPLGIGIATGEQCHNRVIFKQLLQAKALQFLQIDSCRLGSVNENLSVLLMAKKFEIPVCPHAGGVGLCELVQHLIIFDYISVSASLENRVCEYVDHLHEHFKYPVMIQRASYMPPKDPGYSTEMKEESVKKHQYPDGEVWKKLLPAQEN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Xenopus
100
Pig
92
Horse
92
Bovine
92
Sheep
92
Dog
92
Rabbit
83
Zebrafish
75
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7L5Y1 As Substrate

Site PTM Type Enzyme
K82 Ubiquitination
K132 Ubiquitination
S148 Phosphorylation
K170 Ubiquitination
K222 Ubiquitination
T246 Phosphorylation
K266 Ubiquitination
K271 Ubiquitination
K314 Ubiquitination
Y397 Phosphorylation
K425 Ubiquitination
K435 Ubiquitination
K436 Ubiquitination

Research Backgrounds

Function:

Plays a role in the catabolism of L-fucose, a sugar that is part of the carbohydrates that are attached to cellular glycoproteins. Catalyzes the dehydration of L-fuconate to 2-keto-3-deoxy-L-fuconate by the abstraction of the 2-proton to generate an enediolate intermediate that is stabilized by the magnesium ion.

PTMs:

Could be sumoylated.

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the mandelate racemase/muconate lactonizing enzyme family. ENOSF1 subfamily.

Research Fields

· Metabolism > Carbohydrate metabolism > Fructose and mannose metabolism.

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