MFGE8 Antibody - #DF10184

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Product: MFGE8 Antibody
Catalog: DF10184
Description: Rabbit polyclonal antibody to MFGE8
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Xenopus
Mol.Wt.: 43 kDa; 43kD(Calculated).
Uniprot: Q08431
RRID: AB_2840763

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Xenopus(83%)
Clonality:
Polyclonal
Specificity:
MFGE8 Antibody detects endogenous levels of total MFGE8.
RRID:
AB_2840763
Cite Format: Affinity Biosciences Cat# DF10184, RRID:AB_2840763.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BA46; Breast epithelial antigen BA46; EDIL1; HMFG; hP47; HsT19888; Lactadherin; Medin; MFG-E8; MFGE8; MFGM; MFGM_HUMAN; Milk fat globule EGF factor 8; Milk fat globule EGF factor 8 protein; Milk fat globule-EGF factor 8; O acetyl disialoganglioside synthase; OAcGD3S; SED1; SPAG10; Sperm associated antigen 10; Sperm surface protein hP47;

Immunogens

Immunogen:
Uniprot:

Q08431(MFGM_HUMAN) >>Visit HPA database.

Gene(ID):
MFGE8(4240)
Expression:
Q08431 MFGM_HUMAN:

Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.

Sequence:
MPRPRLLAALCGALLCAPSLLVALDICSKNPCHNGGLCEEISQEVRGDVFPSYTCTCLKGYAGNHCETKCVEPLGLENGNIANSQIAASSVRVTFLGLQHWVPELARLNRAGMVNAWTPSSNDDNPWIQVNLLRRMWVTGVVTQGASRLASHEYLKAFKVAYSLNGHEFDFIHDVNKKHKEFVGNWNKNAVHVNLFETPVEAQYVRLYPTSCHTACTLRFELLGCELNGCANPLGLKNNSIPDKQITASSSYKTWGLHLFSWNPSYARLDKQGNFNAWVAGSYGNDQWLQVDLGSSKEVTGIITQGARNFGSVQFVASYKVAYSNDSANWTEYQDPRTGSSKIFPGNWDNHSHKKNLFETPILARYVRILPVAWHNRIALRLELLGC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Xenopus
83
Pig
75
Horse
75
Bovine
75
Sheep
75
Dog
75
Chicken
75
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q08431 As Substrate

Site PTM Type Enzyme
S42 Phosphorylation
K59 Ubiquitination
K69 Ubiquitination
K156 Ubiquitination
N228 N-Glycosylation
N238 N-Glycosylation
T247 Phosphorylation
S249 Phosphorylation
S250 Phosphorylation
Y252 Phosphorylation
N325 N-Glycosylation
N329 N-Glycosylation
N350 N-Glycosylation

Research Backgrounds

Function:

Plays an important role in the maintenance of intestinal epithelial homeostasis and the promotion of mucosal healing. Promotes VEGF-dependent neovascularization (By similarity). Contributes to phagocytic removal of apoptotic cells in many tissues. Specific ligand for the alpha-v/beta-3 and alpha-v/beta-5 receptors. Also binds to phosphatidylserine-enriched cell surfaces in a receptor-independent manner. Zona pellucida-binding protein which may play a role in gamete interaction.

Medin is the main constituent of aortic medial amyloid.

PTMs:

Medin has a ragged N-terminus with minor species starting at Pro-264 and Gly-273.

Subcellular Location:

Membrane>Peripheral membrane protein. Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Mammary epithelial cell surfaces and aortic media. Overexpressed in several carcinomas.

Family&Domains:

The F5/8 type C 2 domain mediates high-affinity binding to phosphatidylserine-containing membranes.

References

1). Eugenol Inhibits Ox-LDL-Induced Proliferation and Migration of Human Vascular Smooth Muscle Cells by Inhibiting the Ang II/MFG-E8/MCP-1 Signaling Cascade. Journal of inflammation research, 2024 (PubMed: 38328560) [IF=4.5]

Application: WB    Species: Human    Sample: HVSMCs

Figure 3. Eugenol inhibits the expression of MFG-E8, thereby inhibiting the proliferation and migration of HVSMCs stimulated by ox-LDL. (A and B) The expression of MFG-E8 was detected using Western blot. (C) MTT assay was used to determine cell viability. (D and E) The expression of PCNA was detected using Western blot. (F and G) Cell cycle was detected using flow cytometry. (H and I) Wound healing experiment. (J and K) Transwell migration experiment. ***Represents P < 0.001 vs control group. #P < 0.05, ##Represents P < 0.01, ###Represents P < 0.001 vs ox-LDL group.

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