CDO1 Antibody - #DF10174

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Product: CDO1 Antibody
Catalog: DF10174
Description: Rabbit polyclonal antibody to CDO1
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 23 kDa; 23kD(Calculated).
Uniprot: Q16878
RRID: AB_2840753

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(91%), Dog(100%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
CDO1 Antibody detects endogenous levels of total CDO1.
RRID:
AB_2840753
Cite Format: Affinity Biosciences Cat# DF10174, RRID:AB_2840753.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CDO 1; CDO; CDO I; CDO-I; Cdo1; CDO1_HUMAN; CDOI; Cysteine dioxygenase type 1; Cysteine dioxygenase type I; Cytosolic cysteine dioxygenase;

Immunogens

Immunogen:
Uniprot:

Q16878(CDO1_HUMAN) >>Visit HPA database.

Gene(ID):
CDO1(1036)
Expression:
Q16878 CDO1_HUMAN:

Highly expressed in liver and placenta. Low expression in heart, brain and pancreas. Also detected in hepatoblastoma Hep-G2 cells.

Sequence:
MEQTEVLKPRTLADLIRILHQLFAGDEVNVEEVQAIMEAYESDPTEWAMYAKFDQYRYTRNLVDQGNGKFNLMILCWGEGHGSSIHDHTNSHCFLKMLQGNLKETLFAWPDKKSNEMVKKSERVLRENQCAYINDSIGLHRVENISHTEPAVSLHLYSPPFDTCHAFDQRTGHKNKVTMTFHSKFGIRTPNATSGSLENN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
91
Xenopus
90
Zebrafish
73
Chicken
73
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q16878 As Substrate

Site PTM Type Enzyme
T4 Phosphorylation
K8 Ubiquitination
T59 Phosphorylation
T193 Phosphorylation
S194 Phosphorylation

Research Backgrounds

Function:

Initiates several important metabolic pathways related to pyruvate and several sulfurate compounds including sulfate, hypotaurine and taurine. Critical regulator of cellular cysteine concentrations. Has an important role in maintaining the hepatic concentation of intracellular free cysteine within a proper narrow range.

PTMs:

The thioether cross-link between Cys-93 and Tyr-157 plays a structural role through stabilizing the Fe(2+) ion, and prevents the production of highly damaging free hydroxyl radicals by holding the oxygen radical via hydroxyl hydrogen.

Tissue Specificity:

Highly expressed in liver and placenta. Low expression in heart, brain and pancreas. Also detected in hepatoblastoma Hep-G2 cells.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the cysteine dioxygenase family.

Research Fields

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Metabolism of other amino acids > Taurine and hypotaurine metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

References

1). RNA sequencing reveals the potential mechanism of exercise preconditioning for cerebral ischemia reperfusion injury in rats. Brain and behavior, 2024 (PubMed: 38956886) [IF=3.1]

Application: WB    Species: Rat    Sample:

FIGURE 7 Exercise preconditioning (EP) suppressed TIMP1, SOCS3, ANGPTL4, CDO1, and SERPINE1 expressions of the cerebral cortices in middle cerebral artery occlusion (MCAO) rats. At 48 h after cerebral ischemia reperfusion injury (CIRI), TIMP1, SOCS3, ANGPTL4, CDO1, and SERPINE1 expression levels of cerebral cortices in each group were evaluated by qPCR (a) and Western blotting (b). # p < .05, ## p < .01 versus Sham. *p < .05, **p < .01 versus EP. Results were presented as mean ± SD. n = 3.

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