Glycophorin A Antibody | Affinity Biosciences

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Western blot analysis of extracts from Rat muscle, using GYPA Antibody.

Product:	Glycophorin A Antibody
Catalog:	DF10170
Description:	Rabbit polyclonal antibody to Glycophorin A
Application:	WB IHC IF/ICC
Reactivity:	Human, Rat
Mol.Wt.:	16 kDa; 16kD(Calculated).
Uniprot:	P02724
RRID:	AB_2840749

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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100ul	$280	In stock
200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Clonality:

Polyclonal

Specificity:

Glycophorin A Antibody detects endogenous levels of total Glycophorin A.

RRID:

AB_2840749
Cite Format: Affinity Biosciences Cat# DF10170, RRID:AB_2840749.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

AI853584; Blood group--MN locus; CD_antigen=CD235a; CD235a; GLPA_HUMAN; Glycophorin A (MNS blood group); Glycophorin A; Glycophorin A, included; Glycophorin-A; GlycophorinA; GPA; GPErik; GpMiIII; GPSAT; GYPA; GYPA, included; HGpMiIII; HGpMiV; HGpMiX; HGpMiXI; HGpSta(C); MN; MN sialoglycoprotein; MNS; PAS-2; PAS2; Sialoglycoprotein alpha;

Immunogens

Immunogen:

Uniprot:

P02724(GLPA_HUMAN) >>Visit HPA database.

Gene(ID):

GYPA(2993)

Sequence:

P02724 GLPA_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MYGKIIFVLLLSEIVSISASSTTGVAMHTSTSSSVTKSYISSQTNDTHKRDTYAATPRAHEVSEISVRTVYPPEEETGERVQLAHHFSEPEITLIIFGVMAGVIGTILLISYGIRRLIKKSPSDVKPLPSPDTDVPLSSVEIENPETSDQ

PTMs - P02724 As Substrate

P02724

Site	PTM Type	Source
S21	O-Glycosylation	Uniprot
T22	O-Glycosylation	Uniprot
T23	O-Glycosylation	Uniprot
T29	O-Glycosylation	Uniprot
S30	O-Glycosylation	Uniprot
T31	O-Glycosylation	Uniprot
S32	O-Glycosylation	Uniprot
T36	O-Glycosylation	Uniprot
S38	O-Glycosylation	Uniprot
S41	O-Glycosylation	Uniprot
T44	O-Glycosylation	Uniprot
N45	N-Glycosylation	Uniprot
T52	O-Glycosylation	Uniprot
T56	O-Glycosylation	Uniprot
S63	O-Glycosylation	Uniprot
S66	O-Glycosylation	Uniprot
T69	O-Glycosylation	Uniprot
S121	Phosphorylation	Uniprot
S130	Phosphorylation	Uniprot
T133	Phosphorylation	Uniprot
S138	Phosphorylation	Uniprot
S139	Phosphorylation	Uniprot
T147	Phosphorylation	Uniprot
S148	Phosphorylation	Uniprot

Research Backgrounds

P02724_GLPA_HUMAN

Function:

Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175); binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV).

PTMs:

The major O-linked glycan are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH (about 78 %) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH (17 %). Minor O-glycans (5 %) include NeuAc-alpha-(2-3)-Gal-beta-(1-3)-[NeuAc-alpha-(2-6)]-GalNAcOH NeuAc-alpha-(2-8)-NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH. About 1% of all O-linked glycans carry blood group A, B and H determinants. They derive from a type-2 precursor core structure, Gal-beta-(1,3)-GlcNAc-beta-1-R, and the antigens are synthesized by addition of fucose (H antigen-specific) and then N-acetylgalactosamine (A antigen-specific) or galactose (B antigen-specific). Specifically O-linked-glycans are NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta-(3-1)-GalNAc-alpha (about 1%, B antigen-specific) and NeuAc-alpha-(2-3)-Gal-beta-(1-3)-GalNAcOH-(6-1)-GlcNAc-beta-(4-1)-[Fuc-alpha-(1-2)]-Gal-beta (1 %, O antigen-, A antigen- and B antigen-specific).

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.
Note: Appears to be colocalized with SLC4A1.

Subunit Structure:

Homodimer.

(Microbial infection) Interacts with Streptococcus gordonii hsa protein.

Family&Domains:

Belongs to the glycophorin A family.

Research Fields

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

References

1). Bioinspired Nanosponge for Salvaging Ischemic Stroke via Free Radical Scavenging and Self-Adapted Oxygen Regulating. NANO LETTERS, 2019 (PubMed: 31830790) [IF=10.8]

Application: WB Species: mouse Sample: erythrocyte

Figure S3. Component analysis of Hb, Mn, total protein in MNET. (A) Hb concentrtion of erythrocyte, NET and MNET (n=3). (B) ICP-MS analysis of Mn amounts in NE and MNE (n=3). (C) The analysis of SDS-PAGE protein electrophoresis of Marker , erythrocyte , NET and MNET. (D) Western blot analysis of Hb, CD41, CD235a and CD47 protein expression in different preparations.

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Glycophorin A Antibody - #DF10170