COLEC12 Antibody - #DF10165

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Product: COLEC12 Antibody
Catalog: DF10165
Description: Rabbit polyclonal antibody to COLEC12
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 82 kDa; 82kD(Calculated).
Uniprot: Q5KU26
RRID: AB_2840744

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(88%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
COLEC12 Antibody detects endogenous levels of total COLEC12.
RRID:
AB_2840744
Cite Format: Affinity Biosciences Cat# DF10165, RRID:AB_2840744.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CL P1; CL-P1; CLP1; COL12_HUMAN; COLEC 12; colec12; Collectin placenta 1; Collectin placenta protein 1; Collectin sub family member 12; Collectin-12; hCL P1; hCL-P1; NSR2; Nurse cell scavenger receptor 2; SCARA4; Scavenger receptor class A member 4; Scavenger receptor with C type lectin; Scavenger receptor with C-type lectin; SRCL;

Immunogens

Immunogen:
Uniprot:

Q5KU26(COL12_HUMAN) >>Visit HPA database.

Gene(ID):
COLEC12(81035)
Expression:
Q5KU26 COL12_HUMAN:

Expressed in perivascular macrophages. Expressed in plaques-surrounding reactive astrocytes and in perivascular astrocytes associated with cerebral amyloid angiopathy (CAA) in the temporal cortex of Alzheimer patient (at protein level). Strongly expressed in placenta. Moderately expressed in heart, skeletal muscle, small intestine and lung. Weakly expressed in brain, colon, thymus and kidney. Expressed in nurse-like cells. Expressed in reactive astrocytes and vascular/perivascular cells in the brain of Alzheimer patient.

Sequence:
MKDDFAEEEEVQSFGYKRFGIQEGTQCTKCKNNWALKFSIILLYILCALLTITVAILGYKVVEKMDNVTGGMETSRQTYDDKLTAVESDLKKLGDQTGKKAISTNSELSTFRSDILDLRQQLREITEKTSKNKDTLEKLQASGDALVDRQSQLKETLENNSFLITTVNKTLQAYNGYVTNLQQDTSVLQGNLQNQMYSHNVVIMNLNNLNLTQVQQRNLITNLQRSVDDTSQAIQRIKNDFQNLQQVFLQAKKDTDWLKEKVQSLQTLAANNSALAKANNDTLEDMNSQLNSFTGQMENITTISQANEQNLKDLQDLHKDAENRTAIKFNQLEERFQLFETDIVNIISNISYTAHHLRTLTSNLNEVRTTCTDTLTKHTDDLTSLNNTLANIRLDSVSLRMQQDLMRSRLDTEVANLSVIMEEMKLVDSKHGQLIKNFTILQGPPGPRGPRGDRGSQGPPGPTGNKGQKGEKGEPGPPGPAGERGPIGPAGPPGERGGKGSKGSQGPKGSRGSPGKPGPQGSSGDPGPPGPPGKEGLPGPQGPPGFQGLQGTVGEPGVPGPRGLPGLPGVPGMPGPKGPPGPPGPSGAVVPLALQNEPTPAPEDNGCPPHWKNFTDKCYYFSVEKEIFEDAKLFCEDKSSHLVFINTREEQQWIKKQMVGRESHWIGLTDSERENEWKWLDGTSPDYKNWKAGQPDNWGHGHGPGEDCAGLIYAGQWNDFQCEDVNNFICEKDRETVLSSAL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Dog
88
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5KU26 As Substrate

Site PTM Type Enzyme
Y16 Phosphorylation
T69 Phosphorylation
T74 Phosphorylation
T78 Phosphorylation
T104 Phosphorylation
S106 Phosphorylation
S109 Phosphorylation
T110 Phosphorylation
K128 Acetylation
K131 Acetylation
R149 Methylation
S398 Phosphorylation
K425 Acetylation
Y687 Phosphorylation

Research Backgrounds

Function:

Scavenger receptor that displays several functions associated with host defense. Promotes binding and phagocytosis of Gram-positive, Gram-negative bacteria and yeast. Mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. Binds to several carbohydrates including Gal-type ligands, D-galactose, L- and D-fucose, GalNAc, T and Tn antigens in a calcium-dependent manner and internalizes specifically GalNAc in nurse-like cells. Binds also to sialyl Lewis X or a trisaccharide and asialo-orosomucoid (ASOR). May also play a role in the clearance of amyloid-beta in Alzheimer disease.

Subcellular Location:

Membrane>Single-pass type II membrane protein.
Note: Forms clusters on the cell surface.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in perivascular macrophages. Expressed in plaques-surrounding reactive astrocytes and in perivascular astrocytes associated with cerebral amyloid angiopathy (CAA) in the temporal cortex of Alzheimer patient (at protein level). Strongly expressed in placenta. Moderately expressed in heart, skeletal muscle, small intestine and lung. Weakly expressed in brain, colon, thymus and kidney. Expressed in nurse-like cells. Expressed in reactive astrocytes and vascular/perivascular cells in the brain of Alzheimer patient.

Subunit Structure:

The extracellular domain forms a stable trimer. The extracellular domain interacts with fibrillar amyloid-beta peptide.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

References

1). Integrated bioinformatics analyses of key genes involved in hepatocellular carcinoma immunosuppression. Oncology Letters, 2021 (PubMed: 34691257) [IF=2.9]

Application: WB    Species: Human    Sample: liver tissues and HCC tissues

Figure 4. Validation of COLEC12, MMP12 and MUC12 mRNA and protein levels. (A) Comparison of COLEC12 mRNA levels between HCC tissues (n=17) and cirrhosis (n=20) or normal liver tissues (n=6) in the GEO datasets (GSE17548 and GSE83148), and immunohistochemical staining of COLEC12 in HCC tissues and normal liver tissues downloaded from the Human Protein Atlas. (B) The comparisons of the MMP12 mRNA level between HCC tissues and cirrhosis or normal liver tissues in the GEO datasets (GSE17548 and GSE83148). (C) The comparisons of the MUC12 mRNA level between HCC tissues and cirrhosis or normal liver tissues in the GEO datasets (GSE17548 and GSE83148), and immunohistochemical staining of MUC12 in HCC tissues and normal liver tissues downloaded from the Human Protein Atlas. (D) The comparisons of the mRNA and protein expression levels of COLEC12, MUC12 and MMP12 between tumor samples (HCC tissues, n=4) and normal (corresponding adjacent normal liver tissues, n=4) samples of HCC patients from the Second Affiliated Hospital of Nanchang University. *P<0.05 and **P<0.01 vs. normal or cirrhosis, respectively. COLEC12, collectin-12 gene, MMP12, matrix metalloproteinase-12 gene; MUC12, mucin-12 gene; HCC, hepatocellular carcinoma; GEO, Gene Expression Omnibus.

Application: WB    Species: Human    Sample: HCC tissues and normal liver tissues

Figure 4. Validation of COLEC12, MMP12 and MUC12 mRNA and protein levels. (A) Comparison of COLEC12 mRNA levels between HCC tissues (n=17) and cirrhosis (n=20) or normal liver tissues (n=6) in the GEO datasets (GSE17548 and GSE83148), and immunohistochemical staining of COLEC12 in HCC tissues and normal liver tissues downloaded from the Human Protein Atlas. (B) The comparisons of the MMP12 mRNA level between HCC tissues and cirrhosis or normal liver tissues in the GEO datasets (GSE17548 and GSE83148). (C) The comparisons of the MUC12 mRNA level between HCC tissues and cirrhosis or normal liver tissues in the GEO datasets (GSE17548 and GSE83148), and immunohistochemical staining of MUC12 in HCC tissues and normal liver tissues downloaded from the Human Protein Atlas. (D) The comparisons of the mRNA and protein expression levels of COLEC12, MUC12 and MMP12 between tumor samples (HCC tissues, n=4) and normal (corresponding adjacent normal liver tissues, n=4) samples of HCC patients from the Second Affiliated Hospital of Nanchang University. *P<0.05 and **P<0.01 vs. normal or cirrhosis, respectively. COLEC12, collectin-12 gene, MMP12, matrix metalloproteinase-12 gene; MUC12, mucin-12 gene; HCC, hepatocellular carcinoma; GEO, Gene Expression Omnibus.

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