PAK6 Antibody - #DF10138

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Product: PAK6 Antibody
Catalog: DF10138
Description: Rabbit polyclonal antibody to PAK6
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Rabbit, Dog
Mol.Wt.: 75 kDa; 75kD(Calculated).
Uniprot: Q9NQU5
RRID: AB_2840718

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Rabbit(86%), Dog(100%)
Clonality:
Polyclonal
Specificity:
PAK6 Antibody detects endogenous levels of total PAK6.
RRID:
AB_2840718
Cite Format: Affinity Biosciences Cat# DF10138, RRID:AB_2840718.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CDKN1A activated kinase 6; EC 2.7.1.37; p21 activated protein kinase 6; p21 protein (Cdc42/Rac)-activated kinase 6; p21(CDKN1A) activated kinase 6; p21-activated kinase 6; p21activated kinase 6; PAK 5; PAK 6; PAK-5; PAK-6; PAK5; Pak6; PAK6_HUMAN; Serine threonine protein kinase PAK 6; Serine/threonine protein kinase PAK 6; Serine/threonine protein kinase PAK6; Serine/threonine-protein kinase PAK 6;

Immunogens

Immunogen:
Uniprot:

Q9NQU5(PAK6_HUMAN) >>Visit HPA database.

Gene(ID):
PAK6(106821730)
Expression:
Q9NQU5 PAK6_HUMAN:

Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast.

Sequence:
MFRKKKKKRPEISAPQNFQHRVHTSFDPKEGKFVGLPPQWQNILDTLRRPKPVVDPSRITRVQLQPMKTVVRGSAMPVDGYISGLLNDIQKLSVISSNTLRGRSPTSRRRAQSLGLLGDEHWATDPDMYLQSPQSERTDPHGLYLSCNGGTPAGHKQMPWPEPQSPRVLPNGLAAKAQSLGPAEFQGASQRCLQLGACLQSSPPGASPPTGTNRHGMKAAKHGSEEARPQSCLVGSATGRPGGEGSPSPKTRESSLKRRLFRSMFLSTAATAPPSSSKPGPPPQSKPNSSFRPPQKDNPPSLVAKAQSLPSDQPVGTFSPLTTSDTSSPQKSLRTAPATGQLPGRSSPAGSPRTWHAQISTSNLYLPQDPTVAKGALAGEDTGVVTHEQFKAALRMVVDQGDPRLLLDSYVKIGEGSTGIVCLAREKHSGRQVAVKMMDLRKQQRRELLFNEVVIMRDYQHFNVVEMYKSYLVGEELWVLMEFLQGGALTDIVSQVRLNEEQIATVCEAVLQALAYLHAQGVIHRDIKSDSILLTLDGRVKLSDFGFCAQISKDVPKRKSLVGTPYWMAPEVISRSLYATEVDIWSLGIMVIEMVDGEPPYFSDSPVQAMKRLRDSPPPKLKNSHKVSPVLRDFLERMLVRDPQERATAQELLDHPFLLQTGLPECLVPLIQLYRKQTSTC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
86
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NQU5 As Substrate

Site PTM Type Enzyme
T69 Phosphorylation
Y81 Phosphorylation
S83 Phosphorylation
T99 Phosphorylation
S104 Phosphorylation
S113 Phosphorylation
Y129 Phosphorylation
S132 Phosphorylation
S135 Phosphorylation
Y144 Phosphorylation
S165 Phosphorylation Q16539 (MAPK14)
S179 Phosphorylation
S207 Phosphorylation
S246 Phosphorylation
S248 Phosphorylation
S308 Phosphorylation
T326 Phosphorylation
S327 Phosphorylation
S328 Phosphorylation
T339 Phosphorylation
S346 Phosphorylation
S347 Phosphorylation
S351 Phosphorylation
T354 Phosphorylation
S360 Phosphorylation
Y365 Phosphorylation
T371 Phosphorylation
K528 Ubiquitination
S560 Phosphorylation Q9NQU5 (PAK6)
T564 Phosphorylation P52564 (MAP2K6)
Y566 Phosphorylation P52564 (MAP2K6)
S616 Phosphorylation

PTMs - Q9NQU5 As Enzyme

Substrate Site Source
P10275 (AR) S579 Uniprot
P53667 (LIMK1) T508 Uniprot
Q00987 (MDM2) T158 Uniprot
Q00987 (MDM2) S186 Uniprot
Q92934 (BAD) S75 Uniprot
Q9BY11 (PACSIN1) S346 Uniprot
Q9NQU5 (PAK6) S560 Uniprot

Research Backgrounds

Function:

Serine/threonine protein kinase that plays a role in the regulation of gene transcription. The kinase activity is induced by various effectors including AR or MAP2K6/MAPKK6. Phosphorylates the DNA-binding domain of androgen receptor/AR and thereby inhibits AR-mediated transcription. Inhibits also ESR1-mediated transcription. May play a role in cytoskeleton regulation by interacting with IQGAP1. May protect cells from apoptosis through phosphorylation of BAD.

PTMs:

Autophosphorylated. Phosphorylated by MAP2K6//MAPKK6, leading to PAK6 activation.

Subcellular Location:

Cytoplasm. Nucleus.
Note: Cotranslocates into nucleus with AR in response to androgen induction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Selectively expressed in brain and testis, with lower levels in multiple tissues including prostate and breast.

Subunit Structure:

Interacts tightly with GTP-bound but not GDP-bound CDC42/p21 and RAC1 (By similarity). Interacts with the androgen receptor AR and the estrogen receptor ESR1. Interacts with IQGAP1 and PPM1B.

Family&Domains:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Focal adhesion.   (View pathway)

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > ErbB signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Human Diseases > Cancers: Specific types > Renal cell carcinoma.   (View pathway)

· Organismal Systems > Development > Axon guidance.   (View pathway)

· Organismal Systems > Immune system > T cell receptor signaling pathway.   (View pathway)

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