SETMAR Antibody - #DF10118
| Product: | SETMAR Antibody |
| Catalog: | DF10118 |
| Description: | Rabbit polyclonal antibody to SETMAR |
| Application: | WB |
| Reactivity: | Human |
| Prediction: | Bovine, Dog |
| Mol.Wt.: | 78 kDa; 78kD(Calculated). |
| Uniprot: | Q53H47 |
| RRID: | AB_2840698 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF10118, RRID:AB_2840698.
Fold/Unfold
Histone lysine N methyltransferase; Histone lysine N methyltransferase SETMAR; Hsmar 1; Hsmar1; Mariner transposase Hsmar1; Metnase; SET domain and mariner transposase fusion; SET domain and mariner transposase fusion gene; SET domain and mariner transposase fusion gene containing protein; SET domain and mariner transposase fusion gene-containing protein; Setmar; SETMR_HUMAN;
Immunogens
Widely expressed, with highest expression in placenta and ovary and lowest expression in skeletal muscle.
- Q53H47 SETMR_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFAEAAKTTRPCGMAEFKEKPEAPTEQLDVACGQENLPVGAWPPGAAPAPFQYTPDHVVGPGADIDPTQITFPGCICVKTPCLPGTCSCLRHGENYDDNSCLRDIGSGGKYAEPVFECNVLCRCSDHCRNRVVQKGLQFHFQVFKTHKKGWGLRTLEFIPKGRFVCEYAGEVLGFSEVQRRIHLQTKSDSNYIIAIREHVYNGQVMETFVDPTYIGNIGRFLNHSCEPNLLMIPVRIDSMVPKLALFAAKDIVPEEELSYDYSGRYLNLTVSEDKERLDHGKLRKPCYCGAKSCTAFLPFDSSLYCPVEKSNISCGNEKEPSMCGSAPSVFPSCKRLTLETMKMMLDKKQIRAIFLFEFKMGRKAAETTRNINNAFGPGTANERTVQWWFKKFCKGDESLEDEERSGRPSEVDNDQLRAIIEADPLTTTREVAEELNVNHSTVVRHLKQIGKVKKLDKWVPHELTENQKNRRFEVSSSLILRNHNEPFLDRIVTCDEKWILYDNRRRSAQWLDQEEAPKHFPKPILHPKKVMVTIWWSAAGLIHYSFLNPGETITSEKYAQEIDEMNQKLQRLQLALVNRKGPILLHDNARPHVAQPTLQKLNELGYEVLPHPPYSPDLLPTNYHVFKHLNNFLQGKRFHNQQDAENAFQEFVESQSTDFYATGINQLISRWQKCVDCNGSYFD
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q53H47 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K275 | Ubiquitination | Uniprot | |
| K335 | Methylation | Uniprot | |
| T338 | Phosphorylation | Uniprot | |
| T341 | Phosphorylation | Uniprot | |
| K343 | Acetylation | Uniprot | |
| K348 | Acetylation | Uniprot | |
| K391 | Acetylation | Uniprot | |
| K395 | Ubiquitination | Uniprot | |
| S410 | Phosphorylation | Uniprot | |
| K448 | Acetylation | Uniprot | |
| K498 | Methylation | Uniprot | |
| S508 | Phosphorylation | O14757 (CHEK1) | Uniprot |
| K523 | Ubiquitination | Uniprot | |
| K569 | Ubiquitination | Uniprot | |
| K637 | Ubiquitination | Uniprot | |
| K674 | Ubiquitination | Uniprot |
Research Backgrounds
Protein derived from the fusion of a methylase with the transposase of an Hsmar1 transposon that plays a role in DNA double-strand break repair, stalled replication fork restart and DNA integration. DNA-binding protein, it is indirectly recruited to sites of DNA damage through protein-protein interactions. Has also kept a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity. In parallel, has a histone methyltransferase activity and methylates 'Lys-4' and 'Lys-36' of histone H3. Specifically mediates dimethylation of H3 'Lys-36' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining. Also regulates replication fork processing, promoting replication fork restart and regulating DNA decatenation through stimulation of the topoisomerase activity of TOP2A.
Methylated. Methylation regulates activity in DNA decatenation.
Phosphorylated at Ser-508 by CHEK1 and dephosphorylated by protein phosphatase 2A/PP2A. Phosphorylation at Ser-508 is enhanced by DNA damage and promotes recruitment to damaged DNA. It stimulates DNA repair and impairs replication fork restart.
Nucleus. Chromosome.
Note: Recruited on damaged DNA at sites of double-strand breaks.
Widely expressed, with highest expression in placenta and ovary and lowest expression in skeletal muscle.
Homodimer. Interacts with PRPF19; required for SETMAR recruitment to damaged DNA sites. Interacts with PCNA. Interacts with TOP2A; stimulates TOP2A topoisomerase activity. May interact with RAD9A and/or RAD9B.
The mariner transposase Hsmar1 region mediates DNA-binding. It has retained some of the nucleases activity but has lost its transposase activity because the active site contains an Asn in position 610 instead of an Asp residue.
In the pre-SET domain, Cys residues bind 3 zinc ions that are arranged in a triangular cluster; some of these Cys residues contribute to the binding of two zinc ions within the cluster.
In the N-terminal section; belongs to the class V-like SAM-binding methyltransferase superfamily.
In the C-terminal section; belongs to the mariner transposase family.
Research Fields
· Metabolism > Amino acid metabolism > Lysine degradation.
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