CYBA Antibody - #DF10099

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Product: CYBA Antibody
Catalog: DF10099
Description: Rabbit polyclonal antibody to CYBA
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 21 kDa; 21kD(Calculated).
Uniprot: P13498
RRID: AB_2840679

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
CYBA Antibody detects endogenous levels of total CYBA.
RRID:
AB_2840679
Cite Format: Affinity Biosciences Cat# DF10099, RRID:AB_2840679.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CYBA; Cytochrome b 245 alpha polypeptide; Cytochrome b 245 light chain; Cytochrome b alpha polypeptide; Cytochrome b(558) alpha chain; Cytochrome b(558) alpha subunit; Cytochrome b558 subunit alpha; Flavocytochrome b 558 alpha polypeptide; Neutrophil cytochrome b 22 kDa polypeptide; p22 phagocyte B-cytochrome; p22-phox; p22phox; Superoxide-generating NADPH oxidase light chain subunit;

Immunogens

Immunogen:
Uniprot:

P13498(CY24A_HUMAN) >>Visit HPA database.

Gene(ID):
CYBA(1535)
Sequence:
MGQIEWAMWANEQALASGLILITGGIVATAGRFTQWYFGAYSIVAGVFVCLLEYPRGKRKKGSTMERWGQKYMTAVVKLFGPFTRNYYVRAVLHLLLSVPAGFLLATILGTACLAIASGIYLLAAVRGEQWTPIEPKPRERPQIGGTIKQPPSNPPPRPPAEARKKPSEEEAAVAAGGPPGGPQVNPIPVTDEVV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
92
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13498 As Substrate

Site PTM Type Enzyme
K60 Ubiquitination
K61 Ubiquitination
K71 Ubiquitination
Y72 Phosphorylation
K78 Ubiquitination
Y87 Phosphorylation
Y88 Phosphorylation
K137 Ubiquitination
T147 Phosphorylation P17252 (PRKCA) , Q05655 (PRKCD)
K149 Ubiquitination
S153 Phosphorylation
S168 Phosphorylation

Research Backgrounds

Function:

Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide.

PTMs:

The heme prosthetic group could be coordinated with residues of the light chain, the heavy chain, or both, and it is possible that more than one heme is present per cytochrome b-245.

Phosphorylation at Thr-147 enhances NADPH oxidase activity by promoting p47phox binding.

Ubiquitinated at Lys-149 likely by RNF145.

Subcellular Location:

Cell membrane.
Note: As unassembled monomer may localize to the endoplasmic reticulum.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Composed of a heavy chain (beta) and a light chain (alpha). Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF1 (via SH3 domain). Interacts with SH3PXD2A (By similarity). Interacts with DUOX1, DUOX2 and TPO. Interacts with NOX3 and NOX4. Interacts with calprotectin (S100A8/9).

Family&Domains:

Belongs to the p22phox family.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Leishmaniasis.

· Organismal Systems > Development > Osteoclast differentiation.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

References

1). ELABELA attenuates deoxycorticosterone acetate/salt-induced hypertension and renal injury by inhibition of NADPH oxidase/ROS/NLRP3 inflammasome pathway. Cell Death & Disease, 2020 (PubMed: 32829380) [IF=9.0]

Application: WB    Species: mouse    Sample: renal cortex

Fig. 5 Effects of ELA on DOCA/salt-induced NADPH oxidase formation and activation. a mRNA levels of p22phox, gp91phox, p47phox, and p67phox subunits of NADPH oxidase in the renal cortex. b mRNA levels of p22phox, gp91phox, p47phox, and p67phox subunits of NADPH oxidase in the renal medulla. c Representative immunoblots of p22phox and p47phox and summarized intensities of blots. d Intracellular NADP+/NADPH in renal cortex and medulla. N = 6 per group. *P < 0.05 versus Ctrl group; #P < 0.05 versus DOCA/salt group.
2). Aqueous extract of Phellinus igniarius ameliorates hyperuricemia and renal injury in adenine/potassium oxonate-treated mice. Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2024 (PubMed: 38879892) [IF=7.5]
3). Protective effect of Berberine on reproductive function and spermatogenesis in diabetic rats via inhibition of ROS/JAK2/NFκB pathway. Andrology, 2020 (PubMed: 32012485) [IF=4.5]

Application: WB    Species: rat    Sample: testis

FIGURE 3|BB attenuates ROS production in the testis of DM rats. (A) Representative Western blot results for NOX5, p22phox, gp91phox, NOX1, NOX3, and NOX4 in the testes of rats from all four groups.
4). Forsythiaside A Regulates Activation of Hepatic Stellate Cells by Inhibiting NOX4-Dependent ROS. Oxidative Medicine and Cellular Longevity, 2022 (PubMed: 35035671)
5). Ficus pandurata Hance Inhibits Ulcerative Colitis and Colitis-Associated Secondary Liver Damage of Mice by Enhancing Antioxidation Activity. Oxidative Medicine and Cellular Longevity, 2021 (PubMed: 34966476)

Application: WB    Species: Mouse    Sample:

Figure 5 FPH enhances the colonic antioxidation in the UC mice induced by DSS. Effect of FPH on the levels of MDA (a), T-SOD (b), and GSH-Px (c) in the colonic homogenate detected by ELISA. (d) Effect of FPH on the protein expressions of Keap1, Nrf2, HO1, NQO1, p22-phox, and NOX2 in colon tissues detected by Western blot. Results were expressed as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01 vs. the control group; #p < 0.05, ##p < 0.01 vs. the model group (DSS only).

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