JDP2 Antibody - #DF10087

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Product: JDP2 Antibody
Catalog: DF10087
Description: Rabbit polyclonal antibody to JDP2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 19 kDa; 19kD(Calculated).
Uniprot: Q8WYK2
RRID: AB_2840667

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
JDP2 Antibody detects endogenous levels of total JDP2.
RRID:
AB_2840667
Cite Format: Affinity Biosciences Cat# DF10087, RRID:AB_2840667.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Jdp2; JDP2_HUMAN; Jun dimerization protein 2; JUNDM2; Progesterone receptor co-activator;

Immunogens

Immunogen:
Uniprot:

Q8WYK2(JDP2_HUMAN) >>Visit HPA database.

Gene(ID):
JDP2(122953)
Sequence:
MMPGQIPDPSVTTGSLPGLGPLTGLPSSALTVEELKYADIRNLGAMIAPLHFLEVKLGKRPQPVKSELDEEEERRKRRREKNKVAAARCRNKKKERTEFLQRESERLELMNAELKTQIEELKQERQQLILMLNRHRPTCIVRTDSVKTPESEGNPLLEQLEKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WYK2 As Substrate

Site PTM Type Enzyme
K65 Sumoylation
T138 Phosphorylation
T143 Phosphorylation
S145 Phosphorylation
T148 Phosphorylation P45983 (MAPK8) , P45984 (MAPK9) , Q16539 (MAPK14)
S151 Phosphorylation

Research Backgrounds

Function:

Component of the AP-1 transcription factor that represses transactivation mediated by the Jun family of proteins. Involved in a variety of transcriptional responses associated with AP-1 such as UV-induced apoptosis, cell differentiation, tumorigenesis and antitumogeneris. Can also function as a repressor by recruiting histone deacetylase 3/HDAC3 to the promoter region of JUN. May control transcription via direct regulation of the modification of histones and the assembly of chromatin.

PTMs:

Phosphorylation of Thr-148 by MAPK8 in response to different stress conditions such as, UV irradiation, oxidatives stress and anisomycin treatments.

Polyubiquitinated; probably by IRF2BP1.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Forms a homodimer or heterodimer with JUN, JUNB, JUND, CEBPG and ATF2 thereby inhibiting transactivation by JUN, ATF2 and CEBPG (By similarity). Binds multiple DNA elements such as cAMP-response element (CRE) and TPA response element (TRE) either as homodimer or heterodimer (By similarity). Interacts with IRF2BP1.

Family&Domains:

Belongs to the bZIP family. ATF subfamily.

References

1). ITGBL1 transcriptionally inhibited by JDP2 promotes the development of pancreatic cancer through the TGF-beta/Smad pathway. BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH, 2022 (PubMed: 35584452) [IF=2.3]

Application: WB    Species: Human    Sample: pancreatic cancer cells

Figure 6 ITGBL1 was transcriptionally inhibited by JDP2 in pancreatic cancer cells and mediated the effects of JDP2 overexpression (OE). A, ITGBL1 mRNA levels in JDP2-overexpressed cells were measured by real-time quantitative PCR. B, JDP2 and ITGBL1 protein levels in pancreatic cancer cells with JDP2 overexpression were measured by western blot. C, Luciferase activity was detected after PANC-1 cells were co-transfected with a luciferase reporter vector and JDP2 overexpression vector or empty vector. D and E, Cell viability, migration (Scale bar=200 μm), and invasion (Scale bar=100 μm) of JDP2-overexpressed cells transfected with ITGBL1 overexpression vector or empty vector were measured. Data are reported as means±SD for N=3 (ANOVA and t-test).

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