TUSC4 Antibody - #DF10069
| Product: | TUSC4 Antibody |
| Catalog: | DF10069 |
| Description: | Rabbit polyclonal antibody to TUSC4 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 44 kDa; 44kD(Calculated). |
| Uniprot: | Q8WTW4 |
| RRID: | AB_2840649 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF10069, RRID:AB_2840649.
Fold/Unfold
2810446G01Rik; G21 protein; Gene 21 protein; Homologous to yeast nitrogen permease (candidate tumor suppressor); Homologous to yeast nitrogen permease; Nitrogen permease regulator 2-like protein; Nitrogen permease regulator-like 2 (S. cerevisiae); NPR 2L; NPR L2; NPR like 2; NPR2; NPR2 like; NPR2-like protein; NPR2L; NPRL 2; NPRL2; NPRL2_HUMAN; Tumor suppressor candidate 4; TUSC 4; TUSC 4 protein; TUSC4; TUSC4 protein;
Immunogens
Most abundant in skeletal muscle, followed by brain, liver and pancreas, with lower amounts in lung, kidney, placenta and heart. Expressed in the frontal lobe cortex as well as in the temporal, parietal, and occipital lobes (PubMed:27173016, PubMed:26505888). Expressed in most lung cancer cell lines tested.
- Q8WTW4 NPRL2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGSGCRIECIFFSEFHPTLGPKITYQVPEDFISRELFDTVQVYIITKPELQNKLITVTAMEKKLIGCPVCIEHKKYSRNALLFNLGFVCDAQAKTCALEPIVKKLAGYLTTLELESSFVSMEESKQKLVPIMTILLEELNASGRCTLPIDESNTIHLKVIEQRPDPPVAQEYDVPVFTKDKEDFFNSQWDLTTQQILPYIDGFRHIQKISAEADVELNLVRIAIQNLLYYGVVTLVSILQYSNVYCPTPKVQDLVDDKSLQEACLSYVTKQGHKRASLRDVFQLYCSLSPGTTVRDLIGRHPQQLQHVDERKLIQFGLMKNLIRRLQKYPVRVTREEQSHPARLYTGCHSYDEICCKTGMSYHELDERLENDPNIIICWK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8WTW4 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K62 | Ubiquitination | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K74 | Ubiquitination | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| K158 | Ubiquitination | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| K258 | Ubiquitination | Uniprot | |
| K270 | Ubiquitination | Uniprot | |
| T293 | Phosphorylation | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| K357 | Ubiquitination | Uniprot |
Research Backgrounds
As a component of the GATOR1 complex functions as an inhibitor of the amino acid-sensing branch of the TORC1 pathway. The GATOR1 complex strongly increases GTP hydrolysis by RRAGA and RRAGB within RRAGC-containing heterodimers, thereby deactivating RRAGs, releasing mTORC1 from lysosomal surface and inhibiting mTORC1 signaling. The GATOR1 complex is negatively regulated by GATOR2 the other GATOR subcomplex in this amino acid-sensing branch of the TORC1 pathway.
Suppresses Src-dependent tyrosine phosphorylation and activation of PDPK1 and its downstream signaling. Down-regulates PDPK1 kinase activity by interfering with tyrosine phosphorylation at 'Tyr-9', 'Tyr-373' and 'Tyr-376' residues. May act as a tumor suppressor. Suppresses cell growth and enhances sensitivity to various anticancer drugs.
Lysosome membrane.
Note: Localization to lysosomes is amino acid-independent.
Most abundant in skeletal muscle, followed by brain, liver and pancreas, with lower amounts in lung, kidney, placenta and heart. Expressed in the frontal lobe cortex as well as in the temporal, parietal, and occipital lobes. Expressed in most lung cancer cell lines tested.
Forms a heterodimer with NPRL3. Interacts with PDPK1. Within the GATOR complex, component of the GATOR1 subcomplex, made of DEPDC5, NPRL2 and NPRL3. GATOR1 mediates the strong interaction of the GATOR complex with RRAGA/RRAGC and RRAGB/RRAGC heterodimers.
Belongs to the NPR2 family.
Research Fields
· Environmental Information Processing > Signal transduction > mTOR signaling pathway. (View pathway)
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