CYFIP1 Antibody - #DF10059

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Product: CYFIP1 Antibody
Catalog: DF10059
Description: Rabbit polyclonal antibody to CYFIP1
Application: WB
Reactivity: Human
Prediction: Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 145 kDa; 145kD(Calculated).
Uniprot: Q7L576
RRID: AB_2840639

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Zebrafish(80%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(90%)
Clonality:
Polyclonal
Specificity:
CYFIP1 Antibody detects endogenous levels of total CYFIP1.
RRID:
AB_2840639
Cite Format: Affinity Biosciences Cat# DF10059, RRID:AB_2840639.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CY; CYFIP 1; CYFIP1; CYFP1_HUMAN; Cytoplasmic FMR1 interacting protein 1; Cytoplasmic FMR1-interacting protein 1; FLJ45151; KIAA0068; p140sra 1; p140sra-1; P140SRA1; Selective hybridizing clone; SHYC; Specifically Rac1 associated protein 1; Specifically Rac1-associated protein 1; SRA 1; Sra-1; SRA1;

Immunogens

Immunogen:
Uniprot:

Q7L576(CYFP1_HUMAN) >>Visit HPA database.

Gene(ID):
CYFIP1(23191)
Sequence:
MAAQVTLEDALSNVDLLEELPLPDQQPCIEPPPSSLLYQPNFNTNFEDRNAFVTGIARYIEQATVHSSMNEMLEEGQEYAVMLYTWRSCSRAIPQVKCNEQPNRVEIYEKTVEVLEPEVTKLMNFMYFQRNAIERFCGEVRRLCHAERRKDFVSEAYLITLGKFINMFAVLDELKNMKCSVKNDHSAYKRAAQFLRKMADPQSIQESQNLSMFLANHNKITQSLQQQLEVISGYEELLADIVNLCVDYYENRMYLTPSEKHMLLKVMGFGLYLMDGSVSNIYKLDAKKRINLSKIDKYFKQLQVVPLFGDMQIELARYIKTSAHYEENKSRWTCTSSGSSPQYNICEQMIQIREDHMRFISELARYSNSEVVTGSGRQEAQKTDAEYRKLFDLALQGLQLLSQWSAHVMEVYSWKLVHPTDKYSNKDCPDSAEEYERATRYNYTSEEKFALVEVIAMIKGLQVLMGRMESVFNHAIRHTVYAALQDFSQVTLREPLRQAIKKKKNVIQSVLQAIRKTVCDWETGHEPFNDPALRGEKDPKSGFDIKVPRRAVGPSSTQLYMVRTMLESLIADKSGSKKTLRSSLEGPTILDIEKFHRESFFYTHLINFSETLQQCCDLSQLWFREFFLELTMGRRIQFPIEMSMPWILTDHILETKEASMMEYVLYSLDLYNDSAHYALTRFNKQFLYDEIEAEVNLCFDQFVYKLADQIFAYYKVMAGSLLLDKRLRSECKNQGATIHLPPSNRYETLLKQRHVQLLGRSIDLNRLITQRVSAAMYKSLELAIGRFESEDLTSIVELDGLLEINRMTHKLLSRYLTLDGFDAMFREANHNVSAPYGRITLHVFWELNYDFLPNYCYNGSTNRFVRTVLPFSQEFQRDKQPNAQPQYLHGSKALNLAYSSIYGSYRNFVGPPHFQVICRLLGYQGIAVVMEELLKVVKSLLQGTILQYVKTLMEVMPKICRLPRHEYGSPGILEFFHHQLKDIVEYAELKTVCFQNLREVGNAILFCLLIEQSLSLEEVCDLLHAAPFQNILPRVHVKEGERLDAKMKRLESKYAPLHLVPLIERLGTPQQIAIAREGDLLTKERLCCGLSMFEVILTRIRSFLDDPIWRGPLPSNGVMHVDECVEFHRLWSAMQFVYCIPVGTHEFTVEQCFGDGLHWAGCMIIVLLGQQRRFAVLDFCYHLLKVQKHDGKDEIIKNVPLKKMVERIRKFQILNDEIITILDKYLKSGDGEGTPVEHVRCFQPPIHQSLASS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Sheep
100
Dog
100
Rabbit
100
Chicken
90
Zebrafish
80
Pig
0
Bovine
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q7L576 As Substrate

Site PTM Type Enzyme
K97 Ubiquitination
Y108 Phosphorylation
K110 Ubiquitination
S154 Phosphorylation
K182 Acetylation
K182 Ubiquitination
K189 Acetylation
K197 Acetylation
K197 Ubiquitination
Y254 Phosphorylation
T256 Phosphorylation
K260 Ubiquitination
K294 Ubiquitination
Y298 Phosphorylation
K320 Ubiquitination
S322 Phosphorylation
K329 Ubiquitination
Y366 Phosphorylation
S367 Phosphorylation
T420 Phosphorylation
K422 Ubiquitination
K516 Ubiquitination
K540 Ubiquitination
K546 Ubiquitination
Y560 Phosphorylation
S568 Phosphorylation
K573 Ubiquitination
S574 Phosphorylation
T579 Phosphorylation
S582 Phosphorylation
S583 Phosphorylation
K715 Ubiquitination
K725 Ubiquitination
K751 Ubiquitination
T769 Phosphorylation
K778 Ubiquitination
S872 Phosphorylation
K879 Ubiquitination
Y887 Phosphorylation
S900 Phosphorylation
K935 Ubiquitination
Y967 Phosphorylation
Y986 Phosphorylation
K1038 Ubiquitination
S1052 Phosphorylation
K1053 Ubiquitination
Y1054 Phosphorylation
T1068 Phosphorylation
K1083 Ubiquitination
K1188 Ubiquitination
K1197 Acetylation
K1197 Ubiquitination
K1210 Ubiquitination
K1227 Ubiquitination
T1234 Phosphorylation
S1253 Phosphorylation

Research Backgrounds

Function:

Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA (By similarity). Regulates formation of membrane ruffles and lamellipodia. Plays a role in axon outgrowth. Binds to F-actin but not to RNA. Part of the WAVE complex that regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. Regulator of epithelial morphogenesis. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes (By similarity). May act as an invasion suppressor in cancers.

Subcellular Location:

Cytoplasm. Cytoplasm>Perinuclear region. Cell projection>Lamellipodium. Cell projection>Ruffle. Cell junction>Synapse>Synaptosome.
Note: Highly expressed in the perinuclear region (By similarity). Enriched in synaptosomes (By similarity). Also enriched in membrane ruffles and at the tips of lamellipodia (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. Component of the CYFIP1-EIF4E-FMR1 complex which is composed of CYFIP, EIF4E and FMR1. Interacts with FMR1 but does not bind to related proteins FXR1 or FXR2. Interaction with EIF4E stimulates FMR1 binding. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 (NCKAP1l/HEM1 in hematopoietic cells) and WASF2/WAVE2. Interacts with the active GTP-bound form of RAC1. Interacts through its C-terminus with the C-terminus of DPYSL2/CRMP2 which is necessary for DPYSL2-induced axon outgrowth. Interacts with NYAP1, NYAP2 and MYO16. Interacts with TMEM108 (via N-terminus); the interaction associates TMEM108 with the WAVE1 complex (By similarity).

Family&Domains:

Belongs to the CYFIP family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Genetic Information Processing > Translation > RNA transport.

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