ATOH8 Antibody - #DF10047

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Product: ATOH8 Antibody
Catalog: DF10047
Description: Rabbit polyclonal antibody to ATOH8
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Pig, Bovine, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 35 kDa; 35kD(Calculated).
Uniprot: Q96SQ7
RRID: AB_2840627

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC : 1:50-200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
ATOH8 Antibody detects endogenous levels of total ATOH8.
RRID:
AB_2840627
Cite Format: Affinity Biosciences Cat# DF10047, RRID:AB_2840627.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

4933425C05Rik; ATOH8; ATOH8_HUMAN; Atonal homolog 6; atonal homolog 8 (Drosophila); Atonal homolog 8; Basic helix loop helix transcription factor 6; bHLHa21; Class A basic helix-loop-helix protein 21; FLJ14708; FLJ38730; hATH6; Helix loop helix protein mATH-6; Helix-loop-helix protein hATH-6; Math6; Okadin; Protein atonal homolog 8; RGD1561512;

Immunogens

Immunogen:
Uniprot:

Q96SQ7(ATOH8_HUMAN) >>Visit HPA database.

Gene(ID):
ATOH8(84913)
Expression:
Q96SQ7 ATOH8_HUMAN:

Expressed in lung, liver, kidney, heart and pancreas. Expressed in endothel of umbilical vessels.

Sequence:
MKHIPVLEDGPWKTVCVKELNGLKKLKRKGKEPARRANGYKTFRLDLEAPEPRAVATNGLRDRTHRLQPVPVPVPVPVPVAPAVPPRGGTDTAGERGGSRAPEVSDARKRCFALGAVGPGLPTPPPPPPPAPQSQAPGGPEAQPFREPGLRPRILLCAPPARPAPSAPPAPPAPPESTVRPAPPTRPGESSYSSISHVIYNNHQDSSASPRKRPGEATAASSEIKALQQTRRLLANARERTRVHTISAAFEALRKQVPCYSYGQKLSKLAILRIACNYILSLARLADLDYSADHSNLSFSECVQRCTRTLQAEGRAKKRKE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Chicken
92
Zebrafish
75
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96SQ7 As Substrate

Site PTM Type Enzyme
K24 Acetylation
K25 Acetylation
K27 Acetylation
K29 Acetylation
T245 Phosphorylation
S247 Phosphorylation

Research Backgrounds

Function:

Transcription factor that binds a palindromic (canonical) core consensus DNA sequence 5'-CANNTG- 3' known as an E-box element, possibly as a heterodimer with other bHLH proteins. Regulates endothelial cell proliferation, migration and tube-like structures formation. Modulates endothelial cell differentiation through NOS3. May be implicated in specification and differentiation of neuronal cell lineages in the brain (By similarity). May participate in kidney development and may be involved in podocyte differentiation (By similarity). During early embryonic development is involved in tissue-specific differentiation processes that are dependent on class II bHLH factors and namely modulates the differentiation program initiated by the pro-endocrine factor NEUROG3 (By similarity). During myogenesis, may play a role during the transition of myoblasts from the proliferative phase to the differentiation phase (By similarity). Positively regulates HAMP transcription in two ways, firstly by acting directly on the HAMP promoter via E-boxes binding and indirectly through increased phosphorylation of SMAD protein complex. Repress NEUROG3-dependent gene activation in a gene-specific manner through at least two mechanisms; requires only either the sequestering of a general partner such as TCF3 through heterodimerization, either also requires binding of the bHLH domain to DNA via a basic motif (By similarity).

Subcellular Location:

Nucleus. Nucleus speckle. Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in lung, liver, kidney, heart and pancreas. Expressed in endothel of umbilical vessels.

Subunit Structure:

Efficient DNA binding requires dimerization with another bHLH protein. Interacts with NEUROG3 AND NEUROD1. Interacts with ZFPM2; mediates indirect interaction with GATA4. Forms a heterodimer with TCF3; repress transcription of TCF3 and TCF3/NEUROG3 dimer-induced transactivation of E box-dependent promoters.

Family&Domains:

The bHLH domain mediates transcriptional repression by inhibiting TCF3 transcriptional activity through heterodimerization.

References

1). Shear stress activates ATOH8 via autocrine VEGF promoting glycolysis dependent-survival of colorectal cancer cells in the circulation. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH, 2020 (PubMed: 32000836) [IF=11.3]

Application: IF/ICC    Species: Human    Sample: SW480 cells

Fig. 1 ATOH8 is a shear stress responsive molecule in mimic circulating colorectal cancer cells. a, b Left, representative immunofluorescence images of ATOH8 expression in suspended LoVo and SW480 cells treated with size gradient (0, 5, 10, 20 dyn/cm2 ; 30 min) (a) and time gradient (10 dyn/cm2 ; 0, 15, 30, 60 min) (b) laminar shear stress (LSS). Right, quantification of single tumour cell fluorescence intensity in various treatment groups was presented. c-f Western blotting (WB) and quantitative polymerase chain reaction (qPCR) analysis of ATOH8 expression in suspended LoVo and SW480 cells treated with size gradient (0, 5, 10, 20 dyn/cm2 ; 30 min) (c, d) and time gradient (10 dyn/cm2 ; 0, 15, 30, 60 min) (e, f) LSS. g Immunofluorescence representation images of different subtypes of CTCs in colorectal cancer patients. Red represents epithelial CTCs, green represents mesenchymal CTC, red and green represent mixed CTC, and purple shade represents different expression levels of ATOH8 in CTCs. h In colorectal cancer patients with hypertension, the number of ATOH8 (+) CTCs (CTCs with ATOH8 expression, including ATOH8 low, moderate and high expression) were higher. i Representative immunohistochemistry images of ATOH8, HK2, GLUT1 and LDHA expression in serial sections of mouse subcutaneous tumour tissue and colon cancer lung metastases. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001

Application: WB    Species: Human    Sample: SW480 cells

Fig. 1 ATOH8 is a shear stress responsive molecule in mimic circulating colorectal cancer cells. a, b Left, representative immunofluorescence images of ATOH8 expression in suspended LoVo and SW480 cells treated with size gradient (0, 5, 10, 20 dyn/cm2 ; 30 min) (a) and time gradient (10 dyn/cm2 ; 0, 15, 30, 60 min) (b) laminar shear stress (LSS). Right, quantification of single tumour cell fluorescence intensity in various treatment groups was presented. c-f Western blotting (WB) and quantitative polymerase chain reaction (qPCR) analysis of ATOH8 expression in suspended LoVo and SW480 cells treated with size gradient (0, 5, 10, 20 dyn/cm2 ; 30 min) (c, d) and time gradient (10 dyn/cm2 ; 0, 15, 30, 60 min) (e, f) LSS. g Immunofluorescence representation images of different subtypes of CTCs in colorectal cancer patients. Red represents epithelial CTCs, green represents mesenchymal CTC, red and green represent mixed CTC, and purple shade represents different expression levels of ATOH8 in CTCs. h In colorectal cancer patients with hypertension, the number of ATOH8 (+) CTCs (CTCs with ATOH8 expression, including ATOH8 low, moderate and high expression) were higher. i Representative immunohistochemistry images of ATOH8, HK2, GLUT1 and LDHA expression in serial sections of mouse subcutaneous tumour tissue and colon cancer lung metastases. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001
2). ATOH8 Expression Is Regulated by BMP2 and Plays a Key Role in Human Endometrial Stromal Cell Decidualization. Endocrinology, 2024 (PubMed: 38060684) [IF=3.8]

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