RBAK Antibody - #AF0422

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Product: RBAK Antibody
Catalog: AF0422
Description: Rabbit polyclonal antibody to RBAK
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep
Mol.Wt.: 85kDa; 83kD(Calculated).
Uniprot: Q9NYW8
RRID: AB_2834340

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(80%), Bovine(82%), Horse(91%), Sheep(82%)
Clonality:
Polyclonal
Specificity:
RBAK Antibody detects endogenous levels of total RBAK.
RRID:
AB_2834340
Cite Format: Affinity Biosciences Cat# AF0422, RRID:AB_2834340.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

hRBaK; Hypothetical protein RBAK; RB associated KRAB zinc finger; RB-associated KRAB repressor; RB-associated KRAB zinc finger protein; RBAK; RBAK_HUMAN; Zinc finger protein 769; ZNF 769; ZNF769;

Immunogens

Immunogen:
Uniprot:

Q9NYW8(RBAK_HUMAN) >>Visit HPA database.

Gene(ID):
RBAK(57786)
Expression:
Q9NYW8 RBAK_HUMAN:

Expressed in bone, brain, heart, kidney, liver, lung, pancreas and placenta.

Description:
RBAK May repress E2F-dependent transcription. May promote AR- dependent transcription. Belongs to the krueppel C2H2-type zinc-finger protein family. Interacts with AR and RB1. May also interact with other nuclear hormone receptors such as NR3C1/GR.
Sequence:
MNTLQGPVSFKDVAVDFTQEEWQQLDPDEKITYRDVMLENYSHLVSVGYDTTKPNVIIKLEQGEEPWIMGGEFPCQHSPEAWRVDDLIERIQENEDKHSRQAACINSKTLTEEKENTFSQIYMETSLVPSSIIAHNCVSCGKNLESISQLISSDGSYARTKPDECNECGKTYHGEKMCEFNQNGDTYSHNEENILQKISILEKPFEYNECMEALDNEAVFIAHKRAYIGEKPYEWNDSGPDFIQMSNFNAYQRSQMEMKPFECSECGKSFCKKSKFIIHQRAHTGEKPYECNVCGKSFSQKGTLTVHRRSHLEEKPYKCNECGKTFCQKLHLTQHLRTHSGEKPYECSECGKTFCQKTHLTLHQRNHSGERPYPCNECGKSFSRKSALSDHQRTHTGEKLYKCNECGKSYYRKSTLITHQRTHTGEKPYQCSECGKFFSRVSYLTIHYRSHLEEKPYECNECGKTFNLNSAFIRHRKVHTEEKSHECSECGKFSQLYLTDHHTAHLEEKPYECNECGKTFLVNSAFDGHQPLPKGEKSYECNVCGKLFNELSYYTEHYRSHSEEKPYGCSECGKTFSHNSSLFRHQRVHTGEKPYECYECGKFFSQKSYLTIHHRIHSGEKPYECSKCGKVFSRMSNLTVHYRSHSGEKPYECNECGKVFSQKSYLTVHYRTHSGEKPYECNECGKKFHHRSAFNSHQRIHRRGNMNVLDVENL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
91
Bovine
82
Sheep
82
Pig
80
Dog
64
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NYW8 As Substrate

Site PTM Type Enzyme
Y33 Phosphorylation
S78 Phosphorylation
K97 Sumoylation
K108 Sumoylation
K108 Ubiquitination
K161 Sumoylation
K259 Sumoylation
K275 Sumoylation
T284 Phosphorylation
K301 Sumoylation
K315 Sumoylation
K318 Sumoylation
K357 Sumoylation
S368 Phosphorylation
S386 Phosphorylation
S389 Phosphorylation
T394 Phosphorylation
T396 Phosphorylation
K399 Ubiquitination
T422 Phosphorylation
T424 Phosphorylation
K427 Sumoylation
K427 Ubiquitination
K455 Ubiquitination
K509 Ubiquitination
K534 Sumoylation
K537 Ubiquitination
S560 Phosphorylation
S562 Phosphorylation
K565 Sumoylation
Y567 Phosphorylation
S570 Phosphorylation
T590 Phosphorylation
Y595 Phosphorylation
Y598 Phosphorylation
Y609 Phosphorylation
S618 Phosphorylation
S633 Phosphorylation
T639 Phosphorylation
Y642 Phosphorylation
K649 Ubiquitination
K658 Sumoylation
K663 Sumoylation
K677 Ubiquitination

Research Backgrounds

Function:

May repress E2F-dependent transcription. May promote AR-dependent transcription.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in bone, brain, heart, kidney, liver, lung, pancreas and placenta.

Subunit Structure:

Interacts with AR and RB1. May also interact with other nuclear hormone receptors such as NR3C1/GR.

Family&Domains:

Belongs to the krueppel C2H2-type zinc-finger protein family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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