SEC22B Antibody - #DF10010

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Product: SEC22B Antibody
Catalog: DF10010
Description: Rabbit polyclonal antibody to SEC22B
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 25 kDa; 25kD(Calculated).
Uniprot: O75396
RRID: AB_2840590

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:1000-3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
SEC22B Antibody detects endogenous levels of total SEC22B.
RRID:
AB_2840590
Cite Format: Affinity Biosciences Cat# DF10010, RRID:AB_2840590.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ERS 24; ERS24; SEC22 vesicle trafficking protein homolog B; SEC22 vesicle trafficking protein like 1; SEC22B; SEC22L 1; Vesicle trafficking protein SEC22b;

Immunogens

Immunogen:
Uniprot:

O75396(SC22B_HUMAN) >>Visit HPA database.

Gene(ID):
SEC22B(9554)
Sequence:
MVLLTMIARVADGLPLAASMQEDEQSGRDLQQYQSQAKQLFRKLNEQSPTRCTLEAGAMTFHYIIEQGVCDLVLCEAAFPKTLAFAYLEDLHSEFDEQHGKKVPTVSRPYSFIEFDTFIQKTKKLYIDSCARRNLGSINTELQDVQRIMVANIEEVLQRGEALSALDSKANNLSSLSKKYRQDAKYLNMHSTYAKLAAVAVFFIMLIVYVRFWWL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O75396 As Substrate

Site PTM Type Enzyme
T5 Phosphorylation
S19 Phosphorylation
S26 Phosphorylation
Y33 Phosphorylation
S35 Phosphorylation
K38 Acetylation
K38 Ubiquitination
S48 Phosphorylation
T50 Phosphorylation
S93 Phosphorylation
K102 Ubiquitination
S107 Phosphorylation
Y110 Phosphorylation
S111 Phosphorylation
K121 Ubiquitination
S137 Phosphorylation
T140 Phosphorylation
S164 Phosphorylation
S168 Phosphorylation
K169 Ubiquitination
S174 Phosphorylation
S175 Phosphorylation
S177 Phosphorylation
K178 Ubiquitination
K179 Ubiquitination
K185 Ubiquitination
T192 Phosphorylation
Y193 Phosphorylation

Research Backgrounds

Function:

SNARE involved in targeting and fusion of ER-derived transport vesicles with the Golgi complex as well as Golgi-derived retrograde transport vesicles with the ER.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass type IV membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane. Golgi apparatus>cis-Golgi network membrane. Golgi apparatus>trans-Golgi network membrane. Melanosome.
Note: Concentrated most in the intermediate compartment/cis-Golgi network and the cis-Golgi cisternae 1 and 2. Greatly reduced in concentration at the trans end of the Golgi apparatus (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with STX17 (By similarity). Component of two distinct SNARE complexes consisting of STX5, GOSR2/BOS1, BET1 and SEC22B or STX18, USE1L, BNIP1/SEC20L and SEC22B. YKT6 can probably replace SEC22B in either complex. Interacts with the COPII Sec23/24 complex composed of SEC23A and SEC24A; recruits SEC22B into COPII-coated vesicles to allow its transport from the endoplasmic reticulum to the Golgi.

Family&Domains:

Belongs to the synaptobrevin family.

Research Fields

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

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